1992
DOI: 10.1002/bms.1200211107
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Gas chromatographic/mass spectrometric analysis of stable isotopes of 3-methylhistidine in biological fluids: Application to plasma kineticsin vivo

Abstract: A simple and rapid method for measuring 3-methylhistidine (3MH) in plasma and urine is described. Internal standard, 1-methylhistidine (1MH), was added to plasma, acidified and absorbed onto cation-exchange columns. It was then eluted from columns, dried, and derivatized for gas chromatography/mass spectrometry. A major fragment of 3MH was monitored at 238 u and 3-methyl-(methyl-2H3)histidine (d3-3MH) (used for in vivo kinetics) at 241 u, whereas 1MH was monitored at 340 u and eluted 0.5 min later than 3MH. St… Show more

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Cited by 32 publications
(22 citation statements)
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“…Urine was collected for a 24-h period immediately following a 24-h acclimation period of rats to the metabolic cages. Quantitation of urine 3-MH was by gas chromatography-mass spectrometry (GC-MS) was as described (1,60). Briefly, the internal standard 3-methyl-[methyl-2 H3]-histidine was added to urine and hydrolyzed for 15 h at 110°C and then isolated on cation exchange columns.…”
Section: Methodsmentioning
confidence: 99%
“…Urine was collected for a 24-h period immediately following a 24-h acclimation period of rats to the metabolic cages. Quantitation of urine 3-MH was by gas chromatography-mass spectrometry (GC-MS) was as described (1,60). Briefly, the internal standard 3-methyl-[methyl-2 H3]-histidine was added to urine and hydrolyzed for 15 h at 110°C and then isolated on cation exchange columns.…”
Section: Methodsmentioning
confidence: 99%
“…Protein degradation was measured by urinary 3-methylhistidine (3-MH) on consecutive 24-h urine collections, as described by Rathmacher et al (19).…”
Section: Methodsmentioning
confidence: 99%
“…Contractile protein degradation was assessed by measurement of 3-methylhistidine (3-MH) release from EDL and SOL muscles incubated in vitro. The amount of 3-MH in the incubation medium of the isolated muscles was determined according to a modified GC-MS method (Metabolic Technologies, Ames, IA) previously described by Rathmacher et al (22). Briefly, 1 ml of incubation medium and 10 nmol of 3-[methyl-…”
Section: Methodsmentioning
confidence: 99%
“…The major ion fragments for 3-MH and d3-3-MH were monitored using selective ion monitoring. 3-MH was monitored at 238 atomic mass units and the stable isotope (internal standard) d3-3-MH at 241 atomic mass units, as previously reported (22). Quantitation of 3-MH was performed by determination of the isotope ratios of the unknowns compared with the internal standard, and the standard curves were generated using known 3-MH quantities.…”
Section: Methodsmentioning
confidence: 99%