Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor

Neal, Craig J.; Gupta, Ankur; Barkam, Swetha; Saraf, Shashank; Cho, Hyoung J.; Seal, Sudipta

doi:10.1038/s41598-017-01356-5

“…In conclusion, the recent literature offers multiple examples that reveal H 2 O 2 as a versatile mediator of molecular communication in plants and whether we classify it as a phytohormone or not, this does not change its importance in the life of plants. There are new perspectives emerging in the field of H 2 O 2 research with tools being developed for the detection of low micromolar and even picomolar H 2 O 2 concentrations [ 249 , 250 ] and it is likely that their eventual application in plant sciences will provide answers to some of our questions about H 2 O 2 transport and concentration dynamics. Similarly, we may expect that increasing sensitivity in proteomics approaches combined with imaging or laser microdissection techniques (e.g., [ 251 ]) will reveal more H 2 O 2 targets and their spatio-temporal distribution.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks

Černý

¹

,

Habánová

²

,

Berka

³

et al. 2018

IJMS

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Hydrogen peroxide (H2O2) is steadily gaining more attention in the field of molecular biology research. It is a major REDOX (reduction–oxidation reaction) metabolite and at high concentrations induces oxidative damage to biomolecules, which can culminate in cell death. However, at concentrations in the low nanomolar range, H2O2 acts as a signalling molecule and in many aspects, resembles phytohormones. Though its signalling network in plants is much less well characterized than are those of its counterparts in yeast or mammals, accumulating evidence indicates that the role of H2O2-mediated signalling in plant cells is possibly even more indispensable. In this review, we summarize hydrogen peroxide metabolism in plants, the sources and sinks of this compound and its transport via peroxiporins. We outline H2O2 perception, its direct and indirect effects and known targets in the transcriptional machinery. We focus on the role of H2O2 in plant growth and development and discuss the crosstalk between it and phytohormones. In addition to a literature review, we performed a meta-analysis of available transcriptomics data which provided further evidence for crosstalk between H2O2 and light, nutrient signalling, temperature stress, drought stress and hormonal pathways.

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“…Further, most of the existing methods demonstrate H 2 O 2 measurement in buffer, serum, or urine and direct measurement of H 2 O 2 from the blood sample is not available. Further, externally added H 2 O 2 in serum separated from blood manually is measured using the electrochemical method that involves manual steps 22,24 , and measurements of endogenous H 2 O 2 is not available. Also, a major disadvantage of the electrochemical method is the fouling of electrodes when used with biological samples, which reduces accuracy and sensitivity of measurement with time.…”

Section: Resultsmentioning

confidence: 99%

“…The electrochemical methods that are based on the electron transfer are preferred over the conventional methods owing to the ease of operation and integration. However, such methods have not been applied so far to the human blood sample and inherent disadvantages such as high overpotential 23 and slow electron transfer 24 leading to low sensitivity, poor selectivity, and metal fouling. Electrochemical methods based on the horseradish peroxidase (HRP) enzyme for catalytic decomposition of peroxide are more sensitive and are widely preferred 22,24 .…”

mentioning

confidence: 99%

“…However, such methods have not been applied so far to the human blood sample and inherent disadvantages such as high overpotential 23 and slow electron transfer 24 leading to low sensitivity, poor selectivity, and metal fouling. Electrochemical methods based on the horseradish peroxidase (HRP) enzyme for catalytic decomposition of peroxide are more sensitive and are widely preferred 22,24 . A nanochannel platform involving HRP enzyme and carbodiimide coupling chemistry for the detection of H 2 O 2 was reported 23 .…”

mentioning

confidence: 99%

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Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Gaikwad

¹

,

Thangaraj

²

,

Sen

³

2021

Sci Rep

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The levels of hydrogen peroxide ($${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 ) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in plasma in the concentration range of 0–49 µM, offering a limit of detection of 0.05 µM, a sensitivity of 0.60 µM−1, and detection time of 15 min; the device is amenable to the real-time measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in the patient’s blood. Using the linear correlation obtained with known quantities of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 , the endogenous $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 concentration in the blood of healthy individuals is found to be in the range of 0.8–6 µM. The availability of this device at the point of care will have relevance in understanding the role of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in health and disease.

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“…This nano CeO 2 film was synthesized by a sol‐gel method, and the detection of OTA was studied electrochemically with sensitivity reported as 0.0127 μA ng −1 mL cm −2 . The sensor for non‐enzymatic detection of hydrogen peroxide at the very low detection limit of picomolar concentration was studied using CeO 2 Nps based sensing platform and found implantable biomedical device applications . These nanobiosensors offer a good choice for the detection of biological materials but have some drawback.…”

Section: Introductionmentioning

confidence: 99%

Ceria‐Nanoparticles‐Based Microfluidic Nanobiochip Electrochemical Sensor for the Detection of Ochratoxin‐A

Dhiman

¹

,

Lakshmi

²

,

Roychoudhury

³

et al. 2019

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Ochratoxin-A (OTA) is a fungal mycotoxin that is present in most of the food stuff, and shows adverse effects on human beings. Therefore, the rapid and accurate detection of OTA is necessary. Here, ceria nanoparticles based microfluidic nanobiochip was fabricated for the electrochemical detection of OTA. Ceria nanoparticles were synthesized using a chemical coprecipitation method and characterized by various techniques. Raman spectroscopy confirmed the formation of fluorite cubic structure. Transmission electron microscopy (TEM) exhibited the formation of very small size (4-5 nm) with nearly spherical shape nanoparticles. Microfluidic nanobiochip was fabricated using 300 μm microfluidic channel using polydimethylsiloxane (PDMS). Simultaneously, indium tin oxide (ITO) coated Corning glass was used for the fabrication of immunoelectrodes platform using antibodies (anti-OTA) for the OTA detection. The detection range was achieved from 350 pg ml À 1 to 10 ng ml À 1 with sensitivity of 7.5 μA ng À 1 mL cm À 2 , without any interference effect using differential pulse voltammetry.

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Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor

Cited by 34 publications

References 67 publications

Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks

Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks

Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Ceria‐Nanoparticles‐Based Microfluidic Nanobiochip Electrochemical Sensor for the Detection of Ochratoxin‐A

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