Picomolar analysis of flavins in biological samples by dynamic pH junction-sweeping capillary electrophoresis with laser-induced fluorescence detection

Britz‐McKibbin, Philip; Markuszewski, Michał J.; Iyanagi, Takashi; Matsuda, Keiko; Nishi­oka, Takaaki; Terabe, Shigeru

doi:10.1016/s0003-2697(02)00510-9

Cited by 79 publications

(42 citation statements)

References 27 publications

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“…These two approaches have subsequently been used for the separation of environmentally relevant aromatic amines [177], ephera alkaloids in herbal extracts [178] and naphthalene-2,3-dicarboxaldehyde-derivatised amino acids [179] The other method that has been successfully integrated with sweeping is a dynamic pH junction. This was first demonstrated by Britz-McKibbin et al [180][181][182] for the Reproduced from [176], with permission.…”

Section: Sequential Stacking Methods Featuring Sweepingmentioning

confidence: 87%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in-line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10,000- to 100,000-fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.

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Section: Sequential Stacking Methods Featuring Sweepingmentioning

confidence: 87%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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“…Based on SDS-PAGE analysis, the purified enzymes bound CaM (17-18 kDa) in a ratio of 1:1 and had an estimated molecular mass of about 80 and 74 kDa for the human nNOS and iNOS flavin domains, respectively. In addition, both the purified flavin domains contained FAD and FMN with a ratio of 1:1 determined by capillary electrophoresis (36). The enzymes were further purified by a DE-52 column prior to experiments.…”

Section: Methodsmentioning

confidence: 99%

Mechanistic Studies on the Intramolecular One-electron Transfer between the Two Flavins in the Human Neuronal Nitric-oxide Synthase and Inducible Nitric-oxide Synthase Flavin Domains

Guan

Kamatani

Kimura

et al. 2003

Journal of Biological Chemistry

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“…Recent applications of CE to the analytical determination of vitamins have concentrated on riboflavin and its related flavins, flavin mononucleotide, and flavin adenine dinucleotide [28][29][30][31][32]. Flavins are natively fluorescent and water-soluble, which makes them ideal candidates for CE analysis.…”

Section: Vitaminsmentioning

confidence: 99%

“…One approach reported for flavins has been an on-line preconcentration technique combining dynamic pH junction and sweeping modes of focusing [31,32]. This technique focuses a large sample volume of up to 22% capillary length prior to separation and results in at least 1200-fold improvement in sensitivity over conventional sample injection [31].…”

Section: Vitaminsmentioning

confidence: 99%

Recent advances in the application of capillary electrophoresis for food analysis

Frazier

Papadopoulou

2003

Electrophoresis

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This article reviews recent developments in the application of capillary electrophoresis (CE) for the analysis of foods and food components. CE has been applied to a number of important areas of food analysis and is fast becoming an established technique within food analytical and research laboratories. Papers are reviewed that were published during the two years to date following the previous review (Electrophoresis 2001, 22, 4197-4206).

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Picomolar analysis of flavins in biological samples by dynamic pH junction-sweeping capillary electrophoresis with laser-induced fluorescence detection

Cited by 79 publications

References 27 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

Mechanistic Studies on the Intramolecular One-electron Transfer between the Two Flavins in the Human Neuronal Nitric-oxide Synthase and Inducible Nitric-oxide Synthase Flavin Domains

Recent advances in the application of capillary electrophoresis for food analysis

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