Pico liter dispenser with 128 independent nozzles for high throughput biochip fabrication

Takagi, Fumio; Kurosawa, R.; Sawaki, D.; Kamisuki, S.; Takai, Madoka; Ishihara, K.; Atobe, Masakazu

doi:10.1109/mems.2004.1290576

Cited by 14 publications

(9 citation statements)

References 7 publications

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“…Using this configuration, many droplets of different solutions can be dispensed simultaneously. For example, Takagi et al 81 developed a dispenser having 128 separate nozzles. Each nozzle is independently actuated by an electrostatically moving membrane, which generates a pressure pulse.…”

Section: Inkjet Printingmentioning

confidence: 99%

Bio-Microarray Fabrication Techniques—A Review

Barbulovic-Nad

Lucente

Sun

et al. 2006

Critical Reviews in Biotechnology

347

256

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Microarrays with biomolecules (e.g., DNA and proteins), cells, and tissues immobilized on solid substrates are important tools for biological research, including genomics, proteomics, and cell analysis. In this paper, the current state of microarray fabrication is reviewed. According to spot formation techniques, methods are categorized as "contact printing" and "non-contact printing." Contact printing is a widely used technology, comprising methods such as contact pin printing and microstamping. These methods have many advantages, including reproducibility of printed spots and facile maintenance, as well as drawbacks, including low-throughput fabrication of arrays. Non-contact printing techniques are newer and more varied, comprising photochemistrybased methods, laser writing, electrospray deposition, and inkjet technologies. These technologies emerged from other applications and have the potential to increase microarray fabrication throughput; however, there are several challenges in applying them to microarray fabrication, including interference from satellite drops and biomolecule denaturization.

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Section: Inkjet Printingmentioning

confidence: 99%

Bio-Microarray Fabrication Techniques—A Review

Barbulovic-Nad

Lucente

Sun

et al. 2006

Critical Reviews in Biotechnology

347

256

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“…Addressing N bits jets elements switch circuit system is using in inkjet chip. Logic functions integrated, enabling TIJ-based products have become increasingly high-end market [17][18][19][20][21][22][23]. Inkjet printers can put small number of DNA droplets (usually only a few picoliters, 10-12 l) accurately sprayed on to DNA glasses media.…”

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Printed DNA Droplets Arrangement and Detection Inkjet System

Liou¹

2016

Printed Electronics - Current Trends and Applications

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This article describes the aims to establish a thermal bubble printhead with simultaneously driving multi-channel for DNA droplet arrangement. It proposed a monolithic CMOS/MEMS system with multi-level output voltage ESD protection system for protected inkjet printhead. High-voltage power, low-voltage logic, and CMOS/MEMS architecture were integrated in inkjet chip. It used bulk micromachining technology (MEMS). On-chip high-voltage electrostatic discharge (HV-ESD), protection design in smart power technology of monolithic inkjet chip is a challenging issue. The nozzle jets interleaving scanning sequence is controlled spatially on the elements to avoid the strong interference with DNA droplets caused by the excitation of the neighbor driven elements. A heating element, disposed on the substrate, includes a conductor loop which does not encompass the heating elements on the substrate. The configuration of the heater jet significantly reduces both electromagnetic and capacitance interference caused by the heating elements. The simulation and experience result have shown in the research. It is reduced nearly half the time compared to the case with traditional scanning sequence. This experiment develops new controlled structure designs of chip for inkjet printheads. A bubble inkjet(TIJ) device is designed, several of the architectures may be adjusted just a small microns to improve and optimize the DNA drop nucleation and generation efficiency. The DNA droplet ejection behavior of the multiplexer inkjet printhead within 60-μm orifice size has been measured beyond 5 kHz operation system, 12 pL capacity of ejected DNA droplet volume.

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“…Noncontact dispensing devices usually employ mechanical actuation to eject droplets through small nozzles. Presently, noncontact sys-tems utilize mainly the following actuation techniques: thermal actuation [4]- [6], pneumatic actuation [7], [8], electrostatic actuation [9], [10], acoustic actuation [11], [12], and piezoactuation [13]- [20]. There is a need for high-accuracy and highdensity microdroplet generators and for an understanding of the mechanics of droplet generators [14], [21]- [25].…”

Section: Introductionmentioning

confidence: 99%

“…Nakamura et al [26] showed that both piezoelectric and electrostatic actuation are suitable for biological sample printing. A number of research groups have presented piezoactuated [13]- [17] and electrostaticactuated [10] microdispensers for microarray printing. The piezoactuated dispensing head presented in this paper can be fabricated using low-cost microfabrication processes.…”

Section: Introductionmentioning

confidence: 99%

A Piezoactuated Droplet-Dispensing Microfluidic Chip

Ahamed

Gubarenko

Ben-Mrad

et al. 2010

J. Microelectromech. Syst.

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A microfluidic dispensing device that is capable of generating droplets with volumes varying between 1 nL and 50 pL at an ejection frequency of up to 6 kHz is presented. In this device, a piezoactuator pushes onto an elastic membrane via piston tips; the mechanical bending of the membrane generates a pressure pulse pushing droplets out. An analytical model was developed solving bending characteristics of a plate-actuated fluidic dispensing system and used to calculate the displaced volume. The model was extended to perform stress analysis to find the optimum piston tip radius by minimizing design stresses. The optimum piston tip radius was found to be 67% of the chamber radius. The actuation force estimated using the analytical model was then used as input to a finite element model of the dispenser. A detailed numerical analysis was then performed to model the fluid flow and droplet ejection process and to find critical geometric and operating parameters. Results from both models were used together to find the best design parameters. The device contains three layers, a silicon layer sandwiched between two polydimethylsiloxane (PDMS) polymer layers. Silicon dry etching, together with PDMS soft lithography, was used to fabricate the chip. PDMS oxygen plasma bonding is used to bond the layers. Prototypes developed were successfully tested to dispense same-sized droplets repeatedly without unwanted droplets. The design allows easy expansion and simultaneous dispensing of fluids.

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Pico liter dispenser with 128 independent nozzles for high throughput biochip fabrication

Cited by 14 publications

References 7 publications

Bio-Microarray Fabrication Techniques—A Review

Bio-Microarray Fabrication Techniques—A Review

Design and Fabrication of Printed DNA Droplets Arrangement and Detection Inkjet System

A Piezoactuated Droplet-Dispensing Microfluidic Chip

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