Piceatannol extends the lifespan of Caenorhabditis elegans via DAF‐16

Abstract

Piceatannol is a natural stilbene with many beneficial effects, such as antioxidative, anti-inflammatory, antiatherogenic activities; however, its role on aging is not known. In this study, we used Caenorhabditis elegans as an animal model to study the effect of piceatannol on its lifespan and investigated the underlying mechanisms. The results showed that 50 and 100 µM piceatannol significantly extended the lifespan of C. elegans without altering the growth rate, worm size and progeny production. Piceatannol delayed the age-related decline of pumping rate and locomotive activity, and protected the worms from heat and oxidative stress. This study further indicated that lifespan extension and enhanced stress resistance induced by piceatannol requires DAF-16. Since DAF-16 is conserved from nematodes to mammals, our study may have important implications in utilizing piceatannol to promote healthy aging and combat age-related disease in humans. © 2016 BioFactors, 43(3):379-387, 2017.

p‐Coumaric acid improves oxidative and osmosis stress responses in Caenorhabditis elegans

“…For the SKN‐1 translocation study, nematodes challenged with 2% NaN 3 for 10 min were used as the positive control . More than 50 pictures were taken for each group using confocal microscopy (Nikon Eclipse 80i Microscope SOP, Tokyo, Japan) and analyzed with a chi 2 test as previously reported . The distribution of DAF‐16::GFP and SKN‐1::GFP was identified by three principles: ‘cytoplasmic’ represents animals with no visible nuclear translocation; ‘medium’ represents nematodes with nuclear translocation that is only visible in part of the worms' body; and ‘high’ represents worms with nuclear translocation throughout the whole body (Fig.…”

“…Real time PCR was performed as previously described . After two days' p ‐coumaric acid treatment, nematodes (N2) were collected and their total RNA was extracted by Trizol.…”

“…Real time PCR was performed as previously described. 20 After two days' p-coumaric acid treatment, nematodes (N2) were collected and their total RNA was extracted by Trizol. cDNA was generated by a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific Inc, Middletown, VA, USA).…”

Antioxidant Capacity of Thistle (Cirsium japonicum) in Various Drying Methods and their Protection Effect on Neuronal PC12 cells and Caenorhabditis elegans

“…The chemicals used for the C. elegans experiments were from Thermo Fisher Scientific, Inc., (Pittsburgh, PA, USA), unless indicated otherwise. All experimental methods for C. elegans were adapted from Shen et al [28] and modified. CJ was added to the synchronized L1 worms in 12-well plates.…”

Royalactin induces copious longevity via increased translation and proteasome activity in C. elegans

“…Total reproductive capacity or the temporal pattern thereof are often 39,40 -though certainly not always [e.g. 41 ] -altered in long-lived C. elegans. For royalactin, it would be reasonable to predict a positive effect on growth, reproduction and lifespan alike, since this is typically seen in long-lived castes of social insects 4 .…”