Phytotoxic Analysis of Extract of Leaves of Solanum megalochiton Mart. Solanaceae on Lactuca sativa L. and Allium cepa L.

The objective of this study was to evaluate the effects of the ethanolic crude extracts and fractions of the species Senecio westermanii Dusén on Lactuca sativa L. (lettuce) and Allium cepa L. (onion) seeds. We assessed the germination, growth, root respiration and photosynthesis of the target species in Petri dishes (9.0 cm diameter) containing filter paper n°6. The study was conducted using 50 seeds per plate and held in 4 replicates per concentration of each sample. In the germination there was an inhibitory effect of fractions hexane (FH) and chloroform (FCl) at concentrations of 500 and 1000 µg/mL. There was a reduction in the radicle growth of lettuce by 14 to 24% and a reduction of hypocotilum by 14 to 28%. As for the radicle of the onion was up 74% reduction to the FCl and the coleoptile was 24 and 45% reduction for FH and FCl, respectively. Inhibitory effects in the root respiration of lettuce were detected in all the samples analyzed, with results ranging from 16 to 83%. For the seeds of A. cepa, there was an encouragement for the FCl and ethyl acetate fractions (FAE), with results ranging from 94 to 142% and 76 to 150%, respectively. With regard to the photosynthesis of L. sativa, there was no significant difference between the control, and as for the A. cepa, there was a strain in inhibition concentrations of 250 and 500 µg/mL, which ranged from 27 to 68%. The samples of S. westermanii caused changes in the target species and thus can be used as a natural herbicide.

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Phytotoxic and Enzymatic Study of Philodendron meridionale on seeds of Lactuca sativa L.

Swiech

Folquitto

Bobek

et al. 2021

RSD

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In an effort to identify novel biopesticides, the present study aimed to assess the effects of Philodendron meridionale (Buturi & Sakur) stem and leaf ketonic and ethanolic extracts (SKE, SEE, LKE, and LEE, respectively) on the germination, growth, root respiration, and enzymatic activities of Lactuca sativa L. seeds, and to measure the associated saponins, phenolics, and flavonoids that may possess herbicidal, pharmaceutical, or pesticidal activities. The extracts were obtained using a modified Soxhlet apparatus and methanolic extracts of 0.1, 0.25, 0.5, 0.75 and 1.0 mg.mL-1 were tested, with water and methanol as a control. The presence of saponins and the concentration of phenolic compounds were determined. Allelopathic activity was evaluated in tests of germination, growth, cellular respiration and enzymatic activity. The presence of saponins and the concentration of phenolic compounds equivalent to 225.12 for LKE, 240.45 for LEE, 193.28 for SKE, and 265.14 for SEE (mg·g-1.gallic acid), and flavonoids 52.74 for LKE, 54.31 for LEE, 72.74 for SKE, and 67.21 for SEE (mg.g-1.quercetin) were determined. The allelopathy of the P. meridionale extracts against L. sativa was confirmed through negative effects on L. sativa seed germination, radical growth and morphology, dry mass, and the concentrations of α-amylase (EC 3.2.1.1), ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6), and polyphenol oxidase (EC 1.10.3.1). It was likely that the allelopathic action of the P. meridionale extracts was related to its effects on the membrane permeability and oxidative stress of the treated L. sativa seeds. The P. meridionale extracts contained saponins, calcium oxalate crystals, and flavonoids, including phenolic compounds, which are known allelochemicals with herbicidal activities.

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Phytotoxic Analysis of Extract of Leaves of Solanum megalochiton Mart. Solanaceae on Lactuca sativa L. and Allium cepa L.

Cited by 3 publications

References 17 publications

Allelopathy Potential of Weeds Belonging to the Family

Allelopathy Potential of Weeds Belonging to the Family

A study of the phytotoxic effects of the aerial parts of Senecio westermanii Dusén (Asteraceae) on Lactuca sativa L. and Allium cepa L. seeds

Phytotoxic and Enzymatic Study of Philodendron meridionale on seeds of Lactuca sativa L.

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