Phytotherapy of Aeromonas hydrophila-infected Goldfish, Carassius auratus

Harikrishnan, Ramasamy; Moon, Young-Gun; Kim, Man-Chul; Kim, Ju-Sang; Heo, Moon‐Soo; Balasundaram, Chellam; Dharaneedharan, Subramanian

doi:10.1111/j.1749-7345.2010.00380.x

Cited by 21 publications

(14 citation statements)

References 31 publications

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“…In similar studies using other herbs, Abdel‐Tawwab et al () and Abdel‐Tawwab () found that supplementing diets with green tea and American ginseng enhanced the resistance of Nile tilapia against A. hydrophila infection. Harikrishnan et al () treated A. hydrophila ‐infected goldfish, Carassius auratus , with aqueous herbal concoction obtained from three medicinal plants (neem, tulsi, and turmeric), and examined the histopathologic and recovery changes in lateral muscle, gills, liver, and heart tissues. They found that the dip treatment with the concoction (1% herbal concoction dip treatment for 5 min/d daily at 1100 h) restored the histoarchitecture of the altered fish tissues.…”

Section: Discussionmentioning

confidence: 99%

Turmeric Powder, Curcuma longa L., in Common Carp, Cyprinus carpio L., Diets: Growth Performance, Innate Immunity, and Challenge against Pathogenic Aeromonas hydrophila Infection

Abdel‐Tawwab

Abbass

2016

J World Aquaculture Soc

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Microbial diseases cause economic loss in aquaculture on a global scale, and the use of commercial antibiotics for disease treatment produces undesirable side effects. As many medicinal herbs have evolved a potent defense against pathogenic bacteria, there is a growing interest in these herbs as sources for natural antibacterial agents. In this context, this study was undertaken to evaluate the effect of turmeric powder (TP), Curcuma longa, on the performance and innate immunity of common carp, Cyprinus carpio L., and its challenge against Aeromonas hydrophila infection. Fish (1.4 ± 0.06 g) were fed 0.0 (control), 1.0, 2.0, or 5.0 g TP/kg diet up to satiation twice daily for 10 wk. After the feeding trial, fish of each treatment were intraperitoneally injected by pathogenic A. hydrophila, and they were kept under observation for 10 d during which the daily mortality was recorded. The growth‐promoting effect of TP was monitored and observed where fish growth and innate immunity increased significantly as dietary TP levels increased. According to the regression curve, the most suitable TP level for optimal fish growth and health was found to be 2.0 g/kg. On the other hand, TP inclusion in the fish diet did not significantly affect fish body contents. The obtained results indicate that TP supplementation is a promising immunostimulant, which could improve fish performance and innate immunity and prevent common carp aermoniosis at a level of 2.0 g/kg diet.

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Section: Discussionmentioning

confidence: 99%

Turmeric Powder, Curcuma longa L., in Common Carp, Cyprinus carpio L., Diets: Growth Performance, Innate Immunity, and Challenge against Pathogenic Aeromonas hydrophila Infection

Abdel‐Tawwab

Abbass

2016

J World Aquaculture Soc

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“…The administration of plant extracts to fish by immersion (Table ) has been tested in the treatment of diseases as alternative to traditional drugs and the protocols adopted included single or repeated treatments (Harikrishnan, Rani & Balasundaram ; Harikrishnan, Balasundaram & Bhuvaneswari ; Harikrishnan & Balasundaram ; Rajendiran, Natarjan & Subramanian ; Harikrishnan, Balasundaram, Moon, Kim, Kim & Heo ; Harikrishnan, Moon, Kim, Kim & Heo ; Rattanachaikunsopon & Phumkhachorn ). Dip treatment is logistically more practical for a large number of small fish (weighing less than 5 g) but dilution, exposure time and levels of efficacy are usually not well defined for the majority of immunostimulants (Sakai ; Galindo‐Villegas & Hosokawa ).…”

Section: Route Of Plant Administration and Dosagementioning

confidence: 99%

Current research on the use of plant-derived products in farmed fish

2013

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Over the years, aquaculture has shown increasing development in terms of production. However, due to intensive farming practices, infectious diseases represent the main problem in fish farms, causing heavy economic losses. The use of antibiotics for controlling diseases is widely criticized for its negative impact, including selection of antibioticresistant bacterial strains, immunosuppression, environmental pollution and accumulation of chemical residues in fish tissues. On the other hand, though vaccination is the most effective prophylactic method of preventing disease outbreaks, the development of effective formulations is often hindered by high production costs and the antigenic heterogeneity of the microbial strains. Recently, there has been increased interest in the possibility of using medicinal herbs as immunostimulants, capable of enhancing immune responses and disease resistance of cultured fish. Plant-derived products seem to represent a promising source of bioactive molecules, being at the same time readily available, inexpensive and biocompatible. The aim of this article is to provide an overview of recent research dealing with the use of medicinal plants in aquaculture. Special attention is given to the information about the effects of plant extracts/products on fish growth, haematological profiles, immune responses and resistance to infectious diseases.

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“…A. hydrophila (MTCC 646) was obtained from the Institute of Microbial Technology, Government of India, Chandigarh, and maintained in the laboratory under standard conditions (Harikrishnan et al 2003). The identity of A. hydrophila was verified using biochemical tests, as described previously (Harikrishnan et al 2010). Subcultures were maintained on tryptic soy agar (TSA; Himedia) on slopes at 5°C and routinely tested for pathogenesis (Joseph & Carnahan 1994) by inoculation into American eels (Le Sueur) (Davis & Hayasaka 1983).…”

Section: Methodsmentioning

confidence: 99%

“…One promising method of controlling infection in aquaculture is strengthening the defence mechanisms of fish through prophylactic administration of probiotics and herbs (Gibson et al 1997, Harikrishnan et al 2003, 2010, Sharifuzzaman & Austin 2009). Probiotics such as lactic acid bacteria, produce specific compounds such as bacteriocins or bacteriostatic peptides (Klaenhammer 1988) that inhibit the growth of fish pathogens, including Aeromonas hydrophila, Vibrio anguillarum and A. salmonicida (Lewus et al 1991, Gildberg et al 1995, Santos et al 1996, in addition to improving water quality (Skjermo & Vadstein 1999).…”

Section: Introductionmentioning

confidence: 99%

Potential use of probiotic- and triherbal extract-enriched diets to control Aeromonas hydrophila infection in carp

Harikrishnan

Balasundaram²,

Heo

2010

Dis. Aquat. Org.

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This study reports the effect of probiotic-and triherbal extract-enriched diet on Cyprinus carpio (52 ± 2 g, n = 250) inoculated intramuscularly (50 µl) with Aeromonas hydrophila (1.8 × 10 6 CFU ml -1). On Day 6 post-infection, the fish were divided into 4 groups and fed with the basal diet (IU) or with diets supplemented with triherbal extract (H), Sporolac (S) or Lactobacillus (L). A fifth group of non-infected fish fed with the basal diet was used as a control. Haematology and immunology parameters were measured in Weeks 1, 2 and 4. In the H, S, and L groups, white blood cell levels were significantly increased (p < 0.05) throughout the experimental period. In the H and S groups, red blood cell and haemoglobin levels decreased significantly (p < 0.05) in Weeks 1, 2 and 4. In all the diet groups, the haematocrit and mean corpuscular volume did not differ significantly (p > 0.05) in Week 1, and these values remained near those of the control in Week 2. The S and L groups did not exhibit significant changes (p > 0.05) in glucose, and cholesterol levels in Weeks 2 and 4 compared to controls, and the L group also showed no change in total protein. Respiratory burst activity was significantly increased in Weeks 1 to 4 in the H, S and L groups. Serum-mediated killing of Escherichia coli was significantly increased in the L group in Weeks 1 to 4. Mortalities after A. hydrophila challenge were higher in the IU (85%) and H (50%) groups than in the S (45%) and L (35%) groups. Our results show that formulated probiotic-enriched diets can speed up the recovery of ulcerative dermatitis induced by A. hydrophila in carp. KEY WORDS: Aeromonas hydrophila · Cyprinus carpio · Haematology · Triherbal extract · ProbioticsResale or republication not permitted without written consent of the publisher

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Phytotherapy of Aeromonas hydrophila-infected Goldfish, Carassius auratus

Cited by 21 publications

References 31 publications

Turmeric Powder, Curcuma longa L., in Common Carp, Cyprinus carpio L., Diets: Growth Performance, Innate Immunity, and Challenge against Pathogenic Aeromonas hydrophila Infection

Turmeric Powder, Curcuma longa L., in Common Carp, Cyprinus carpio L., Diets: Growth Performance, Innate Immunity, and Challenge against Pathogenic Aeromonas hydrophila Infection

Current research on the use of plant-derived products in farmed fish

Potential use of probiotic- and triherbal extract-enriched diets to control Aeromonas hydrophila infection in carp

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