Phytoremediation and sequestration of soil metals using the CRISPR/Cas9 technology to modify plants: a review

Bhattacharyya, Nirjhar; Anand, Uttpal; Kumar, Ravi; Ghorai, Mimosa; Aftab, Tariq; Jha, Niraj Kumar; Rajapaksha, Anushka Upamali; Bundschuh, Jochen; Bontempi, Elza; Dey, Abhijit

doi:10.1007/s10311-022-01474-1

Cited by 13 publications

(1 citation statement)

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“…Gene editing tools have been applied for genome engineering of plants, animals, and microorganisms for the expression of specific genes (Paço et al 2019 ; Tang et al 2020 ; Nidhi et al 2021 ; Ozyigit et al 2021 ; Bhattacharyya et al 2022 ; Biswas et al 2022 ; Mandal et al 2022 ; Jiang et al 2022 ). With the advent of different types of gene editing tools such as zinc finger proteins, transcription activator-like effector nucleases, and more recently, the clustered regularly interspaced palindromic repeats (CRISPR)/Cas9, the manipulation of organisms has become easier (Jiang et al 2013 ; Gaj et al 2013 ).…”

Section: Modern Biotechnological Methods To Enhance Microplastic Degr...mentioning

confidence: 99%

Biotechnological methods to remove microplastics: a review

et al. 2023

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Microplastics pollution is major threat to ecosystems and is impacting abiotic and biotic components. Microplastics are diverse and highly complex contaminants that transport other contaminants and microbes. Current methods to remove microplastics include biodegradation, incineration, landfilling, and recycling. Here we review microplastics with focus on sources, toxicity, and biodegradation. We discuss the role of algae, fungi, bacteria in the biodegradation, and we present biotechnological methods to enhance degradation, e.g., gene editing tools and bioinformatics.

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Section: Modern Biotechnological Methods To Enhance Microplastic Degr...mentioning

confidence: 99%

Biotechnological methods to remove microplastics: a review

et al. 2023

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Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches

Das

Anand

Pal

et al. 2023

Biotech & Bioengineering

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Vegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make them an important part of our diet. Numerous biotic and abiotic stresses can affect crop growth, quality, and yield. Traditional and modern breeding strategies to improve plant traits are slow and resource intensive. Therefore, it is necessary to find new approaches for crop improvement. Clustered regularly interspaced short palindromic repeats/CRISPR associated 9 (CRISPR/Cas9) is a genome editing tool that can be used to modify targeted genes for desirable traits with greater efficiency and accuracy. By using CRISPR/Cas9 editing to precisely mutate key genes, it is possible to rapidly generate new germplasm resources for the promotion of important agronomic traits. This is made possible by the availability of whole genome sequencing data and information on the function of genes responsible for important traits. In addition, CRISPR/Cas9 systems have revolutionized agriculture, making genome editing more versatile. Currently, genome editing of vegetable crops is limited to a few vegetable varieties (tomato, sweet potato, potato, carrot, squash, eggplant, etc.) due to lack of regeneration protocols and sufficient genome sequencing data. In this article, we summarize recent studies on the application of CRISPR/Cas9 in improving vegetable trait development and the potential for future improvement.

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