Phytoplasma detection in rose shoots propagated in vitro

Kamińska, M.; Podwyszyńska, Małgorzata; Śliwa, H.

doi:10.5586/asbp.2005.023

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Moreover, Ćurković-Perica and Ježić (2010) reported that in Catharanthus roseus (L.) G. Don, none of the quercetin concentrations (10, 100, or 1 mM) showed efficient cleaning of plants infected with phytoplasma. Kamińska et al (2005) also found that in roses the antibiotics baytril and gentamicin were phytotoxic at concentrations of 25 and 50 mg/L, and, furthermore, have not been effective to eliminate phytoplasma.…”

Section: Antibiotic Treatmentsmentioning

confidence: 99%

Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

Carvalho¹,

Oliveira²,

Souza³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus -CsCMV (by ELISA) and Cassava vein mosaic virus -CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 2 M.J.S. Carvalho et al.Genetics and Molecular Research 16 (2): gmr16029556 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease.

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Section: Antibiotic Treatmentsmentioning

confidence: 99%

Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

Carvalho¹,

Oliveira²,

Souza³

et al. 2017

Genet. Mol. Res.

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Phytoplasma diseases and their relationships with insect and plant hosts in Canadian horticultural and field crops

2009

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Phytoplasmas are bacterial plant pathogens consisting of more than 50 phylogenetic groups that cause devastating diseases in various crops worldwide. They are obligate parasites restricted to the phloem tissue of the host plant and are transmitted from plant to plant mostly by leafhoppers (Hemiptera: Cicadellidae). They reproduce within the tissues of their insect vectors and are transferred in the salivary secretions to new host plants during feeding. Phytoplasma epidemiology involves a tritrophic relationship between the pathogen and usually several hosts and vectors. The host-plant range depends on the number of vectors, their feeding habits, and their dispersal pattern. Interactions between phytoplasmas and their vector hosts are complex and influenced by insects' vectoring abilities and the consequences of infection for vectors. In Canada, seven phytoplasma taxa have been detected in various crops. Aster yellows, the primary vector of which is the leafhopper Macrosteles quadrilineatus (Forbes), is the most common and widespread. X-disease, transmitted by at least eight leafhopper species, is economically damaging to all cultivated species of Prunus L. (Rosaceae). Clover proliferation, also transmitted by M. quadrilineatus, is the causal agent of important diseases such as clover proliferation and alfalfa witches' broom. Ash yellows and pear decline have caused economic problems for several decades, while bois noir, a quarantinable disease in Canada, was detected in Ontario and British Columbia for the first time only recently. Because of their cryptic nature, phytoplasmas are difficult to manage; quarantine measures and insecticide sprays remain the most common control measures. However, integrated pest management techniques using beneficial insects, biotechnology, and plant resistance are emerging.

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Phytoplasma detection in rose shoots propagated in vitro

Cited by 2 publications

References 12 publications

Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

Phytoplasma diseases and their relationships with insect and plant hosts in Canadian horticultural and field crops

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