Phytoplankton functional community structure in Argentinian continental shelf determined by HPLC pigment signatures

Vega-Moreno, Daura; Marrero, Jeffrey; Morales, Jesús Chávez; Garcia, Carla; Úbeda, M.G. Villagarcía; Rueda, M. J.; Llinás, O.

doi:10.1016/j.ecss.2012.01.007

Cited by 30 publications

(14 citation statements)

References 55 publications

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“…The MEDI sample was taken in the Alborán Sea, a zone of strong mesoscale variability, in which the influence of the Atlantic jet coming through the Strait of Gibraltar and wind forcing cause coastal upwelling and other hydrographical structures associated with nutrient fertilization and relatively high Chl a concentrations (Tintoré et al 1991, Macías et al 2007). The eutrophic condition of SWAS, already described in previous studies (Gibb et al 2000, Vega-Moreno et al 2012, reflects the strong hydrodynamism of this region, subjected to tidal mixing and the interactions of the Brazil and Malvinas Currents with the waters over the continental shelf. During our study, the influence of the Sub-Antarctic Surface Waters associated with the Malvinas Current were reflected in the temperature and salinity minima ( Fig.…”

Section: Oceanographic Characteristics Of Surface Waterssupporting

confidence: 65%

Size fractionation, chemotaxonomic groups and bio-optical properties of phytoplankton along a transect from the Mediterranean Sea to the SW Atlantic Ocean

Nunes¹,

Pérez²,

Latasa³

et al. 2019

Sci. Mar.

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The relationships between the structure of the phytoplankton community and the bio-optical properties of surface waters were studied during the TransPEGASO cruise along a transect across the Atlantic Ocean that covered seven biogeographical provinces, from the Alborán Sea (SW Mediterranean) to the Patagonian Shelf. We characterized the composition of the phytoplankton community by means of high-performance liquid chromatography and CHEMTAX pigment analyses applied to whole water and two filtration size classes (< 3 and ≥ 3 μm), flow cytometric determinations and microscopic observations. Additionally, the study was complemented by measurements of the absorption of particulate matter and coloured dissolved organic matter (CDOM). The size class distribution of the chlorophyll a (Chl a) obtained from the size-fractionated filtration (SFF) was compared with that resulting from the diagnostic pigment algorithms (VU) developed by Vidussi et al. (2001) and Uitz et al. (2006), and the total Chl a–based expressions (HI) of Hirata et al. (2011). The seven provinces crossed by the transect could be divided into an oligotrophic group with Chl a < 0.25 mg m-3 comprising the tropical and subtropical Atlantic (including the Canary Current Coastal Province), and a eutrophic group (Chl a > 0.5 mg m-3) with a single Mediterranean (MEDI) sample and those from the southwestern Atlantic Shelf (SWAS). According to CHEMTAX, the most important taxa in the tropical and subtropical Atlantic were Prochlorococcus, haptophytes and Synechoccoccus, while the MEDI and SWAS were dominated by diatoms and haptophytes. Both the VU and HI algorithms, which are based on pigment composition or Chl a concentration, predicted for SWAS a high proportion of nano- and microphytoplankton, while the SFF indicated dominance of the < 3 μm size class. In addition, the CHEMTAX results indicated a high average diatom contribution in this province. However, at several SWAS stations with relatively high values of diatom Chl a estimated by CHEMTAX, the microscopic observations found only small concentrations of nano- or microplankton-sized cells. This discrepancy appeared to be due to the presence, confirmed by scanning electron microscopy, of picoplankton-sized cells of the diatom Minidiscus sp. and of Parmales (a group sharing the pigment composition with the diatoms). These findings caution against a routine assignment of diatom pigments to the microplankton size class. The total non-water absorption in the water column was dominated by CDOM. The average contribution of phytoplankton absorption for the different provinces ranged from 19.3% in the MEDI to 45.7% in the SWAS and 47% in the western tropical Atlantic (WTRA). The Chl a–specific phytoplankton absorption [aph*(443), m2 mg-1] was lower in the MEDI and SWAS than in the oligotrophic provinces. aph*(443) was negatively correlated with the first principal component derived from a principal component analysis based on the concentration of the main pigments and was not correlated with indicators of phytoplankton community size structure such as the proportion of Chl a in the < 3 μm class or a size index derived from the VU size class distribution. These findings indicate that the variability observed in aph*(443) was mainly related to differences in pigment composition and possibly to photoacclimation processes, and that any package effects due to cell size were probably masked by other factors, an outcome that may be related to the relatively small influence of size within the narrow range of Chl a concentrations (all ≤ 2.4 mg m-3) considered in our study.

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Section: Oceanographic Characteristics Of Surface Waterssupporting

confidence: 65%

Size fractionation, chemotaxonomic groups and bio-optical properties of phytoplankton along a transect from the Mediterranean Sea to the SW Atlantic Ocean

Nunes¹,

Pérez²,

Latasa³

et al. 2019

Sci. Mar.

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“…This suggests once again that the Canadian High Arctic's phytoplankton community could be acclimated to low light conditions (i.e., epoxidization of DT into DD under low irradiance) [ Demers et al , 1991; Kashino and Kudoh , 2003; Lavaud et al , 2004; Goss and Jackob , 2010]. The xanthophyll cycle (i.e., response to sudden change in irradiance) could be more active in the Hudson Bay system, containing more xanthophyll pigments [ Kashino et al , 2002] and a more adequate nutrients supply [ Dubinsky and Stambler , 2009; Moreno et al , 2012].…”

Section: Resultsmentioning

confidence: 99%

Variability of phytoplankton light absorption in Canadian Arctic seas

2012

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Phytoplankton light absorption spectra (aϕ(λ)) were measured in the Canadian Arctic (i.e., the Amundsen Gulf, Canadian Arctic Archipelago, northern Baffin Bay and the Hudson Bay system) to improve algorithms used in remote‐sensing models of primary production. The absorption by algae, dominated by picophytoplankton (<5 μm), was not the major light absorption factor in the four provinces; the colored dissolved organic matter (CDOM) contributed up to 70% of total light absorption. During the fall, the low total chlorophyll a‐specific aϕ*(443) (aϕ(443)/TChl a) coefficients of the Canadian High Arctic were associated with photoacclimation processes (i.e., the package effect) occurring in light‐limited environments. Low light availability and high proportion of CDOM (absorbing strongly the ultraviolet) seem to allow the growth of phytoplankton with accessory pigments absorbing light at longer wavelengths. The ratio of photoprotective and photosynthetic carotenoids (PPC:PSC) was inversely proportional with the salinity and the cell size, and mostly decreases throughout the Canadian High Arctic during fall. In return, the highest TChla‐specific phytoplankton light absorption coefficients at the blue peak (aϕ*(443)) were observed in the Hudson Bay system from September to October (i.e., fall) as well as in the Amundsen Gulf from May to July (i.e., spring/summer). These results will ultimately allow the accurate monitoring of phytoplankton biomass and productivity evolution that is likely to take place as a result of the fast‐changing Arctic environment.

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“…After extraction, the samples were filtered through a 0.2 µm Teflon syringe filter into vials, injected into a Schimadzu LC-10ADVP (High Performance Liquid Chromatography: HPLC) and analyzed according to van Heukelem and Thomas (van Heukelem and Thomas, 2001), with slight modifications for the local conditions (Schlüter et al ., 2014) to identify and quantify the phytoplankton pigments. The photoprotective pigment index (PI), a measure of the physiological state of the phytoplankton community, was calculated according to Moreno (Moreno et al ., 2012):

PI = true(diadinoxanthin + diatoxanthin + zeaxanthin true) / Chl a

…”

Section: Methodsmentioning

confidence: 99%

Allochthonous matter: an important factor shaping the phytoplankton community in the Baltic Sea

Paczkowska

Rowe

Schlüter³

et al. 2016

J. Plankton Res.

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It is well-known that nutrients shape phytoplankton communities in marine systems, but in coastal waters allochthonous dissolved organic matter (ADOM) may also be of central importance. We studied how humic substances (proxy of ADOM) and other variables influenced the nutritional strategies, size structure and pigment content of the phytoplankton community along a south–north gradient in the Baltic Sea. During the summer, the proportion of mixotrophs increased gradually from the phosphorus-rich south to the ADOM-rich north, probably due to ADOM-fueled microbes. The opposite trend was observed for autotrophs. The chlorophyll a (Chl a): carbon (C) ratio increased while the levels of photoprotective pigments decreased from south to north, indicating adaptation to the darker humic-rich water in the north. Picocyanobacteria dominated in phosphorus-rich areas while nanoplankton increased in ADOM-rich areas. During the winter–spring the phytoplankton biomass and concentrations of photoprotective pigments were low, and no trends with respect to autotrophs and mixotrophs were observed. Microplankton was the dominant size group in the entire study area. We conclude that changes in the size structure of the phytoplankton community, the Chl a:C ratio and the concentrations of photoprotective pigments are indicative of changes in ADOM, a factor of particular importance in a changing climate.

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Phytoplankton functional community structure in Argentinian continental shelf determined by HPLC pigment signatures

Cited by 30 publications

References 55 publications

Size fractionation, chemotaxonomic groups and bio-optical properties of phytoplankton along a transect from the Mediterranean Sea to the SW Atlantic Ocean

Size fractionation, chemotaxonomic groups and bio-optical properties of phytoplankton along a transect from the Mediterranean Sea to the SW Atlantic Ocean

Variability of phytoplankton light absorption in Canadian Arctic seas

Allochthonous matter: an important factor shaping the phytoplankton community in the Baltic Sea

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