Phytol loaded PLGA nanoparticles ameliorate scopolamine-induced cognitive dysfunction by attenuating cholinesterase activity, oxidative stress and apoptosis in Wistar rat

Sathya, S.; Manogari, Boovaragamoorthy Gowri; Kaliannan, Thamaraiselvi; Sethuraman, Vaidevi; Kandasamy, Ruckmani; Devi, Kasi Pandima

doi:10.1080/1028415x.2020.1764290

Cited by 22 publications

(18 citation statements)

References 45 publications

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Section: Discussionmentioning

confidence: 72%

“…Furthermore, recently it has been reported that phytol, can mitigate the scopolamine-induced cognitive deficit by repressing the activity of cholinesterases in the brains of Wistar rats. 58 Similarly, the outcomes of the HPTLC analysis of SV.CR confirmed the presence of oleanolic acid and caffeic acid in the plant. Oleanolic acid (OA), a pentacyclic oleanane triterpenoid, 59 is endowed with the extraordinary antioxidant potential through direct radical-scavenging as well as indirect biological effects.…”

Section: Discussionmentioning

confidence: 72%

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Chemical Profiling and Dose-Dependent Assessment of Fear Reducing and Memory-Enhancing Effects ofSolanum virginianumin Rats

et al. 2021

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The current study was planned to investigate the pharmacological basis of Solanum virginianum extract (SV.CR) pertaining to anxiolytic, antidepressant and memory-enhancing effects in rats. The SV.CR was analyzed in-vitro for phytoconstituents, antioxidant potential and anticholinesterase activity. The rats treated in a dose-dependent manner (25, 50 and 100 mg/kg of SV.CR) were subjected to behavioral tests for anxiety, depression and memory judgment followed by biochemical studies. A notable dose-dependent anxiolytic potential of SV.CR was observed in elevated plus maze and open field tests (P < 0.05). The decreased immobility time of the treated rats in the forced swim test (P < 0.01) unveiled the plant’s potential to reduce depression. Moreover, SV.CR treatment also reversed scopolamine-impaired cognition (P < 0.05) in various deployed memory and learning tasks. Biochemical studies of brain homogenates of SV.CR treated animals demonstrated decreased anticholinesterase activity and lipid peroxidation levels whereas increased levels of superoxide dismutase and glutathione peroxidase (P < 0.05 vs scopolamine group) were noted. The scientific validation of the study supported the use of Solanum virginianum in reducing anxiety, depression and amnesia in experimental models. Phytoconstituents in SV.CR such as oleanolic acid and caffeic acid might have played a significant neuroprotective role via modulation of oxidative stress and neurochemical aspects.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 72%

Chemical Profiling and Dose-Dependent Assessment of Fear Reducing and Memory-Enhancing Effects ofSolanum virginianumin Rats

et al. 2021

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“…As already mentioned, phytol is a poorly water-soluble molecule. Anterior studies have reported encapsulation efficiency about of 90% for phytol in the PLGA nanoparticles [ 29 , 30 , 31 ]. This is an important parameter for characterizing SLN.…”

Section: Resultsmentioning

confidence: 99%

“…The shifts in spectra of formulations can be associated with drug-loading in the SLN. This achievement is so important to explain possible differences among the biological activity of different samples [ 31 , 49 ]. In this context, the experimental data support the expected ability of SLN for loading of poorly soluble diterpene drugs in an aqueous environment, which can be explored as a potential topical drug delivery system [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

“…In the literature, there are few studies that evaluate the advantages of drug delivery systems containing phytol. In previous studies, Sathya et al (2020) [ 29 , 30 , 31 ] evaluated the activity of phytol-loaded poly (lactic-co-glycolic acid) nanoparticles as a possible treatment for Alzheimer’s disease. In addition, studies conducted by Islam et al (2017) evaluated the antioxidant, toxicity, and cytotoxic effect of phytol-loaded nanoemulsions [ 24 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach

et al. 2020

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Phytol is a diterpene alcohol and can be found as a product of the metabolism of chlorophyll in plants. This compound has been explored as a potential antimicrobial agent, but it is insoluble in water. In this study, we describe a novel approach for an interesting anticandidal drug delivery system containing phytol. Different formulations of phytol-loaded solid lipid nanoparticles (SLN) were designed and tested using a natural lipid, 1,3-distearyl-2-oleyl-glycerol (TG1). Different compositions were considered to obtain three formulations with 1:10, 1:5, and 1:3 w/w phytol/TG1 ratios. All the formulations were prepared by emulsification solvent evaporation method and had their physicochemical properties assessed. The biocompatibility assay was performed in the HEK-293 cell line and the antifungal efficacy was demonstrated in different strains of Candida ssp., including different clinical isolates. Spherical and uniform SLN (<300 nm, PdI < 0.2) with phytol-loading efficiency >65% were achieved. Phytol-loaded SLN showed a dose-dependent cytotoxic effect in the HEK-293 cell line. The three tested formulations of phytol-loaded SLN considerably enhanced the minimal inhibitory concentration of phytol against 15 strains of Candida spp. Considering the clinical isolates, the formulations containing the highest phytol/TG1 ratios showed MICs at 100%. Thus, the feasibility and potential of phytol-loaded SLN was demonstrated in vitro, being a promising nanocarrier for phytol delivery from an anticandidal approach.

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Zein/fucoidan‐coated phytol nanoliposome: preparation, characterization, physicochemical stability, in vitro release, and antioxidant activity

Chen,

Wang,

et al. 2024

J Sci Food Agric

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BACKGROUNDTo improve phytol bioavailability, a novel method of magnetic stirring and high‐pressure homogenization (HPH) combination was used to prepare zein/fucoidan‐coated phytol nanoliposomes (P‐NL‐ZF). The characterization, the simulated in vitro digestion, and the antioxidant activity of these phytol nanoliposomes from the different processes have been studied.RESULTSBased on the results of dynamic light scattering (DLS) and gas chromatography–mass spectrometer (GC–MS) analysis, P‐NL‐ZF prepared through the combination of magnetic stirring and HPH exhibited superior encapsulation efficiency at 76.19% and demonstrated exceptional physicochemical stability under a series of conditions, including storage, pH, and ionic in comparison to single method. It was further confirmed that P‐NL‐ZF by magnetic stirring and HPH displayed a uniform distribution and regular shape through transmission electron microscopy (TEM). Fourier‐transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) analysis showed that electrostatic interactions and hydrogen bonding were the primary driving forces for the formation of composite nanoliposomes. Additionally, an in vitro digestion study revealed that multilayer composite nanoliposomes displayed significant and favorable slow‐release properties (58.21%) under gastrointestinal conditions compared with traditional nanoliposomes (82.36%) and free phytol (89.73%). The assessments of chemical and cell‐based antioxidant activities demonstrated that the coating of zein/fucoidan on phytol nanoliposomes resulted in enhanced effectiveness in scavenging activity of ABTS free radical and hydroxyl radical and mitigating oxidative damage to HepG2 cells.CONCLUSIONBased on our studies, the promising delivery carrier of zein/fucoidan‐coated nanoliposomes is contributed to the encapsulation of hydrophobic natural products and enhancement of their biological activity. © 2024 Society of Chemical Industry.

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Phytol loaded PLGA nanoparticles ameliorate scopolamine-induced cognitive dysfunction by attenuating cholinesterase activity, oxidative stress and apoptosis in Wistar rat

Cited by 22 publications

References 45 publications

Chemical Profiling and Dose-Dependent Assessment of Fear Reducing and Memory-Enhancing Effects ofSolanum virginianumin Rats

Chemical Profiling and Dose-Dependent Assessment of Fear Reducing and Memory-Enhancing Effects ofSolanum virginianumin Rats

Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach

Zein/fucoidan‐coated phytol nanoliposome: preparation, characterization, physicochemical stability, in vitro release, and antioxidant activity

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