Phytohabitans kaempferiae sp. nov., an endophytic actinomycete isolated from the leaf of Kaempferia larsenii

Abstract

A novel endophytic actinomycete, designated strain KK1-3 T , which formed single spores and long chains of spores (more than 10 spores) was isolated from surface-sterilized Kaempferia larsenii leaf collected from Ubon Ratchathani province, Thailand. The isolate contained L-lysine, meso-diaminopimelic acid and hydroxyl diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars included glucose, mannose, rhamnose, ribose, galactose and xylose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphoglycolipids. The predominant menaquinones were MK-10(H 8 ), MK-10(H 6 ) and MK-10(H 4 ). The predominant cellular fatty acids were anteiso-C 17 : 0 and iso-C 16 : 0 . The G+C content of the genomic DNA was 71 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain KK1-3 T should be classified as representing a member of the genus Phytohabitans. The similarity values of sequences between this strain and those of the closely related species, Phytohabitans houttuyneae K11-0057 T (99.0 %), Phytohabitans suffuscus K07-0523 T (98.9 %), Phytohabitans flavus K09-0627 T (98.6 %) and Phytohabitans rumicisK11-0047 T (98.1 %) were observed. The DNA-DNA hybridization result and some physiological and biochemical properties indicated that KK1-3 T could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Phytohabitans kaempferiae sp. nov. is proposed. The type strain is strain KK1-3 T (=BCC 66360 T =NBRC 110005 T ).

Biological Potential and Mechanism of Prodigiosin from Serratia marcescens Subsp. lawsoniana in Human Choriocarcinoma and Prostate Cancer Cell Lines

“…Physiological and biochemical characteristics were examined as described previously [ 59 , 60 ]. Each test was performed on three replicates.…”

Endophytic actinomycetes: promising source of novel bioactive compounds

“…Results suggest 44 genera of Actinobacteria were detected in clones while actinomycetes belonging to only 16 genera were isolated. Furthermore, the genus Phytohabitans which presently comprised only five species isolated from plant samples 24,30,38 were detected only in the clone library. Kibdelosporangium strains isolated in the past from both plant roots and rhizospheric soils were not isolated in this case although those 16S rRNA genes were detected (8.8%).…”

Polyketide Synthase and Nonribosomal Peptide Synthetase Gene Clusters in Type Strains of the Genus Phytohabitans

“…During this study, a novel species Phytohabitans kaempferiae was reported, which is an endophytic actinomycete isolated from the leaf of Kaempferia larsenii [24]. Although the whole genome has yet to be sequenced, the analysis will also reveal further potential of the genus because different species, in general, harbor specific PKS and NRPS pathways, as shown in this and our previous studies on actinomycetes [15][16][17].…”