Phytogeographical profiling of ivy leaf (Hedera helix L.)

Безрук, Иван; Marksa, Mindaugas; Georgiyants, Victoriya; Ivanauskas, Liudas; Raudonė, Lina

doi:10.1016/j.indcrop.2020.112713

Cited by 25 publications

(25 citation statements)

References 41 publications

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“…Analysis has been performed using according to Gluschenko, Mykhailenko and Bezruk [9][10][11] with Shimadzu Nexera X2 chromatographic system coupled with the diode-array detector. Column ACE C18 (250 mm × ×4,6 mm with particle size 5m) was used in the analysis.…”

Section: Methodsmentioning

confidence: 99%

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Comparison of components profile in herbal raw material, extract and pharmaceuticals of Hedera Helix

Безрук

Georgiyants

Ivanauskas

2020

ScienceRise: Pharmaceutical Science

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The aim. To investigate both the profile of components and possible difference among herbal raw materials, semi-products and pharmaceuticals of Hedera helix for determination of main standartisation markers. Materials and methods. Investigation of components profile has been performed using the Shimadzu Nexera X2 chromatographic system coupled with a diode-area detector. The ACE C 18 column (250 mm×4.6 mm with particle size 5 m) was used for the separation of components. 0.1 % acetic acid and acetonitrile were used as mobile phase A and B, respectively. Studies have been performed on the leaves, dry extract and capsules of H. helix. Results. The determined profile had no significant variation among samples. It has been presented by 19 various components, such as phenolic acids, flavonoids and triterpene saponins. However, kaempferol, nicotiflorin and t-cinnamic acid were not found in the leaf raw material. Hederacoside C might be highlighted as the main marker of raw materials and products of H. helix due to its significant amount in comparison to other components. Its amount was in the range of 64,80 % up to 71,46 % of the total content of components. Moreover, according to some pharmacological studies, hederacoside C is responsible for pharmaceutical usage of H. helix pharmaceutics. Nevertheless, it is not recommended to standardize the plant-based medicines by one marker, since the pharmaceutical activity of such dosage forms is defined by synergism action of all constituents. Except for hederacoside C significant amounts in comparison to other components were found for chlorogenic acid and 4,5dicaffeoylquinic acid about 5 % and 3 % respectively. Though the latter was found in small concentrations in leaves (0,058 %). This sample had a much higher amount of 3,5-dicaffeoylquinic acid, but in the case of extract and capsules, its content was lower 1,55 % and 0,66 % respectively. Thus, chlorogenic acid has been chosen as a second marker due to its high concentration in all samples and some pharmaceutical activities, such as antioxidant and anti-inflammatory effects. Conclusions. It was found, that standartisation of H. helix products is preferably to perform with determination both hederacoside C and chlorogenic acid. These components were dominant among all components; besides they possess a wide range of pharmaceutical effects. Hence, quantification of hederacoside C and chlorogenic acid is necessary to ensure the high quality of H. helix pharmaceuticals.

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Section: Methodsmentioning

confidence: 99%

“…Test solution of leaves. The solution was prepared as described in Bezruk et al [11]. Briefly, precisely weighed 1.0 g of H. helix leaves were extracted in triplicate with 15 ml of methanol by ultrasound bath for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

Comparison of components profile in herbal raw material, extract and pharmaceuticals of Hedera Helix

Безрук

Georgiyants

Ivanauskas

2020

ScienceRise: Pharmaceutical Science

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“…Measurement of radical scavenging properties of C. speciosus leaves methanol extracts was carried out according to the method described by Bezruk et al [32] and Marksa et al [33]. After PDA detection, the ABTS solution was mixed with the mobile phase carrying the analytes in the reaction coil [32]. Empower Software Chromatographic Manager System (Waters Corporation, Milford, USA) was used to analyze the data.…”

Section: Antioxidant Activity Of C Speciosus Leavesmentioning

confidence: 99%

“…The total flavonoid content in C. speciosus leaves was 1.07 ± 0.02 mg RE/g or 10.62 ± 0.42 μmol/g. The total content of phenolic compounds depends not only on the place of production, but also on the various growing conditions, the time of harvesting or the preparation time of the plant raw material [30,32]. For this experiment, the leaves of C. speciosus were harvested in December in VDU botanical garden of Kaunas, Lithuania.…”

Section: Quantification Of Phenolic Compounds Of Crocus Speciosus Leavesmentioning

confidence: 99%

Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

Мykhailenko¹,

Безрук²,

Volochai³

et al. 2022

Phyton

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The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method, spectrophotometrically. The total amount of flavonoids in C. speciosus leaves was 1.07 ± 0.02 mg RE/g (p < 0.05), the total amount of phenolic compounds was 0.41 ± 0.01 mg GAE/g (p < 0.05). By HPLC-DAD-MS analysis the presence of the mangiferin, chlorogenic acid, isoorientin, kaempferol, hyperoside, and isoquercitin was established for the first time in Crocus leaves. The antiradical activity of C. speciosus leaves extracts was 150.08 ± 4.5 μmol/g (p < 0.05) and its was mainly attributed to phenolic compounds content. The high amounts of flavonoids and antiradical activity in C. speciosus leaves suggests promising phytochemical and pharmacological study of this Crocus species.

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“…biochemistry, genetics, and molecular biology, has contributed to determination of the structure of phytocompounds and their effect on the organism. Bioactive chemical compounds are used as ingredients of medical products applied in supportive therapy of many diseases , Mendel et al 2011, Uddin et al 2011, Pizzorno et al 2016, Bezruk et al 2020a, 2020b. One of the many medicinal plant species, the common ivy (Hedera helix L.), is a source of bioactive substances with healthenhancing properties [Roşca-Casian et al 2017, Shawky andEl Sohafy 2020].…”

Section: Introductionmentioning

confidence: 99%