Phytogenic synthesis of silver nanoparticles, optimization and evaluation of in vitro antifungal activity against human and plant pathogens

Abstract

An attempt was made to synthesis of biocompatible silver nanoparticles from ten different Cassia spp. Among them, Cassia roxburghii aqueous leaf extract supported the synthesis of highly efficient and stable AgNPs. The synthesis of AgNPs was optimized at different physico-chemical condition and highly stable AgNPs were synthesized with 1.0mL of C. roxburghii leaf extract, pH 7.0, 1.0mM AgNO3 and at 37°C. The synthesized AgNPs were characterized by XPS, DLS and ZETA potential. DLS and ZETA potential analysis, the average AgNPs size was 35nm and the zeta potential was -18.3mV. The AgNPs exhibit higher antifungal activity when compared with the conventional antifungal drug amphotericin B against all the tested human fungal pathogens such as Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Penicillium sp., Candida albicans and the plant pathogens such as Rhizoctonia solani, Fusarium oxysporum and Curvularia sp. Scanning electron microscope (SEM) analysis showed distinct structural changes in the cell membranes of C. albicans upon AgNPs treatment. These results suggest that phytosynthesized AgNPs could be used as effective growth inhibitors in controlling various human and plant diseases caused by fungi.

Biosynthesis of Silver Nanoparticles from <i>Allium ampeloprasum</i> Leaves Extract and Its Antifungal Activity

“…against five different human pathogens A. niger, A. fumigatus, A. flavus, Penicillium sp. and Candidaalbicans [44]. These findings have confirmed the results that demonstrated the antifungal activity of AgNPs biosynthesized by lactic acid bacteria against spoilage fungi from genera Fusarium verticillioides, Penicillium expansum, A. flavus, and A. ochraceus [45].…”

Biogenic Synthesis Silver Nanoparticles Using Peltophorum Pterocarpum Leaf Extracts and Its Antimicrobial Efficacy Against Selective Pathogens

“…In the present study, the leaf extracts of different plants were screened for the synthesis of AgNPs. To one ml of different leaf extracts, 9.0 ml of 1 mmol silver nitrate was mixed separately, and the tubes were incubated in dark conditions at room temperature for 24 h. The solution containing plant extracts and silver nitrate was monitored for colour change, precipitation and agglomeration of nanoparticles [22]. During the preliminary investigation, among the different plant extracts tested, the AgNPs synthesized using Peltophorum pterocarpum leaf extracts showed good stability and hence it was utilized for further studies.…”

“…The AgNPs synthesized using Peltophorum pterocarpum leaf extracts were characterized using the UV-Visible spectrophotometer. The freshly synthesized AgNPs using plant extracts was analyzed for their maximum absorption spectra using UV-Visible spectrophotometer (U 2900, Hitachi) in the range of 300 to 700 nm [22].…”

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

“…These nanoparticles were further tested against human and plant pathogenic fungi, and they exhibited excellent result as compared to tested standard drugs. 139 Chandran et al 140 and Li et al 141 reported the synthesis of AgNPs from the leaf extracts of Aloe vera and Capsicum annum plants, respectively. The rapid synthesis of AgNPs using the fruit extract of Carica papaya was demonstrated, and it was found that the synthesized nanoparticles were highly toxic against different multidrug-resistant (MDR) human pathogens.…”