Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L).R.br revealing its biological properties and photocatalytic activity

Abstract

ZnO NPs were efficiently synthesized from the leaves extract of Ipomoea pes-caprae. The work focuses on phytochemical studies, phytomediated synthesis and characterization of ZnO NPs and its biological applications. Phytochemicals present in the extract may be responsible for reducing the metal salts into their respective nanosize particles. The UV-Vis spectrum infers the formation of NPs, with absorbed peak at 322 nm. The XRD pattern depicted significant peaks at 2h positions which confirmed the hexagonal wurtzite structure. FTIR spectrum (ZnO NPs) was recorded to identify the functional group of biomolecules involved in synthesis. ZnO NPs was measured in the range of 2-20 nm by FE-SEM and particle analyses. ZnO NPs in various concentrations revealed potential antioxidant (IC 50 -21.09) and antibacterial activities. Cytotoxicity activity by Vero cell line (MTT assay) was found to be significant. ZnO NPs exhibited concentration-dependent cytotoxic reactivity to Vero cells after 24-h contact. ZnO NPs exhibited efficient dye degradation of methylene blue in the presence of sunlight. Due to the versatile properties and applications of ZnO NPs, these were widely studied by user-friendly approaches being green, simple and economy.& Venugopal Sujatha

Green Synthesis and Characterization of ZnO Nanoparticles Using Pelargonium odoratissimum (L.) Aqueous Leaf Extract and Their Antioxidant, Antibacterial and Anti-inflammatory Activities

“…The XRD diffraction peaks existed at 2θ angles of 31.85°, 34.55°, 36.35°, 47.69°, 56.75°, 63.09°, 66.56°, 68.17°, 69.29°, 72.87° and 77.21° corresponding to lattice planes (100), (002), (101), (102), (110), (103), (200), (112), (201), (004) and (202), respectively [ 72 ]. These peaks are in accordance with those of (JCPDS card No: 36-1451), which is indicating the confirmation of the hexagonal wurtzite structure of ZnO NPs formation [ 73 ]. The average crystalline size (ACS) of biosynthesized ZnO NPs was calculated using Deby-Scherrer’s formula [ 74 ] and the ACS of the ZnO NPs was estimated to be 14 nm, which is derived from the full width at half maximum (FWHM) of the most intense peak corresponding to (101) plane located at 36.35°.…”

Antimicrobial Activity of Lipopeptide Biosurfactants Against Foodborne Pathogen and Food Spoilage Microorganisms and Their Cytotoxicity

“…Nanoparticles have drawn recently great interest due to their various properties (barrier properties, antimicrobial properties, etc…) that make them advantageous in food and food packaging applications ( Bumbudsanpharoke et al, 2015 ), that’s why it seemed necessary to add them here. Data of these preservatives/antioxidants such as formaldehyde (E240) ( Marcsek et al, 2007 ; Boncler et al, 2019 ), Salvia rosmarinus extract (E392) ( Aherne et al, 2007 ; Waller et al, 2016 ), butylhydroxyanisol (BHA) (E320) ( Labrador et al, 2007 ) and biological extracts such as carvacol (the main component of many essential oil) ( Llana-Ruiz-Cabello et al, 2014 ; Mooyottu et al, 2014 ), iturin A (another antifungal lipopeptide produced by B. subtilis ) ( EFSA Panel on Additives and Products or Substances used in Animal Feed [FEEDAP], 2017 ; Zhao et al, 2018 ), Thymus vulgaris oil extract ( Pérez-González et al, 2019 ) and nanoparticles such as ZnO NP ( Song et al, 2014 ; Venkateasan et al, 2017 ) and Ag NP ( Song et al, 2014 ; Kumar et al, 2017 ) have been collected from literature. In conclusion, as shown in Figure 4 , lipopeptides had low toxicity on human cell lines.…”

Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl

“…The results obtained from the study are in corroboration with the findings of other researchers in which ZnO-NPs synthesized using biological route possessed effective antioxidant activity [ 5 , 26 ]. The antioxidant nature of the green synthesized ZnO-NPs may be correlated to the secondary metabolites that act as agents of capping/reducing/stabilizing during nanoparticle synthesis [ 10 , 21 ].…”

“…les 2020, 25, x FOR PEER REVIEW 9 of 16 activity [5,26]. The antioxidant nature of the green synthesized ZnO-NPs may be correlated to the secondary metabolites that act as agents of capping/reducing/stabilizing during nanoparticle synthesis [10,21].…”

“…From the literature, it may be noted that a few studies have been reported on the synthesis of metal nanoparticles from Ipomea spp. which have been evaluated for antioxidant and cytotoxic effects [ 21 ]; however, to date, no report on the phyto-fabrication of ZnO-NPs from Ipomea obscura has been made. Hence, a study on antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from I. obscura aqueous leaf extract was evaluated.…”