Phytoestrogens induce differential effects on both normal and malignant human breast cells<i>in vitro</i>

Fp, Chen; Mh, Chien

doi:10.3109/13697137.2014.937688

Cited by 31 publications

(25 citation statements)

References 27 publications

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“…Thus, the effects of phytoestrogens were mainly proven in human breast cancer cell lines. In addition to other studies, our previous study demonstrated that genistein, resveratrol and quercetin do not stimulate MCF‐7 breast cancer cells and may induce apoptosis even at low concentrations . However, cancers are well known for high heterogeneity .…”

Section: Discussionmentioning

confidence: 76%

“…These phytoestrogens have a lower binding affinity to ER α but also exert partial anti‐estrogenic effect, due to their relative binding preference to ER β . Our previous study demonstrated that the dichotomy of classic ER modulating action induced by these phytoestrogens may result in different effects on mammalian tissue . However, as of now, the effects of phytoestrogen have been mainly proven in human breast cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

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Effects of phytoestrogens on the activity and growth of primary breast cancer cells ex vivo

Chen

Chien

2019

J of Obstet and Gynaecol

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Aim To explore the ex vivo effects of phytoestrogens on primary human breast cancer cells. Methods Breast cancer cells were obtained from patients who underwent primary breast cancer surgery, which were treated with 10−8 M 17β‐estradiol (E2), one of three phytoestrogens (genistein, resveratrol and quercetin, 10−7 M), and a combination of E2 and one of the three phytoestrogens for 48 h. These cells were then extracted for viability and apoptosis assay. The proteins involved in the proliferative and apoptotic pathways were evaluated by western blot analysis. Results Human breast cancer cell viability was inhibited by all phytoestrogens but induced by E2 with or without phytoestrogen. Apoptotic cells, as well as the proteins involved in apoptotic pathway and estrogen receptor (ER) β, were significantly increased in the cells treated with phytoestrogen alone. The use of E2 with or without a phytoestrogen revealed completely opposite results. The proteins involved in the proliferative pathway and ER α expression were all increased in the cultures with E2 with or without phytoestrogens. Conclusion In the presence of E2, these phytoestrogens lose the effects of suppressing breast cancer cells; contrastingly, induce growth stimulatory effects by inhibiting apoptosis and stimulating proliferation in primary breast cancer cells. Thus, the effects of phytoestrogens on breast cancer should be considered as E2 still present in breast cancer tissue.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Effects of phytoestrogens on the activity and growth of primary breast cancer cells ex vivo

Chen

Chien

2019

J of Obstet and Gynaecol

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“…Our previous studies revealed that the distinctive expression of ER α and ER β may be associated with the apoptotic effects of these phytoestrogens (higher affinities for ER β) and the proliferative effect of E 2 in MCF-7 breast cancer cells (13,14). 8 necessary for proliferation and that ER β opposes the proliferative effects exerted by ER α (32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

“…These phytoestrogens have a lower binding affinity to ER α, but also exert partial anti-estrogenic effect due to their relative binding preference to ER β (11, 12). Our previous study demonstrated that the dichotomy of classic ER modulating action induced by phytoestrogens may result in different effects on mammalian tissue (13,14). However, in the presence of endogenous estrogen, the potential effects induced or modulated as a result of these phytoestrogens and their ER binding capabilities is a cause for concern.…”

Section: Introductionmentioning

confidence: 99%

Impact of lower concentrations of phytoestrogens on the effects of estradiol in breast cancer cells

Chern

2015

Climacteric

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“…Evidence from numerous in vitro and in vivo studies has confirmed an ability of phytoestrogens, such as RSV, to modulate various targets and signaling pathways in a wide range of conditions such as cancer or cardiovascular diseases; they also inhibit cell proliferation in breast cancer cells via different mechanisms, including an ERec‐dependent mechanism. Moreover, RSV is able to maintain the survival of normal cells via ERec‐independent or other mechanisms . Despite RSV well‐documented health benefits, its mechanism of action remains controversial.…”

Section: Discussionmentioning

confidence: 99%

ATP2A3 gene as an important player for resveratrol anticancer activity in breast cancer cells

Izquierdo-Torres

Rodrı́guez

Meneses-Morales

et al. 2017

Molecular Carcinogenesis

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The Ca -ATPases from the Sarco/endoplasmic reticulum (SERCA) are fundamental for maintaining intracellular [Ca ] homeostasis by pumping Ca into the endoplasmic reticulum (ER) of eukaryotic cells. SERCA enzymes are encoded by three different genes (ATP2A1-3), whose expression occurs in a tissue and development stage-specific manner. It has been reported alterations in the expression of SERCA2 and SERCA3 pumps in different types of cancer: oral, lung, colon, stomach, central nervous system, thyroid, breast, and prostate. Resveratrol (RSV), a phytoalexin produced by a wide variety of plants in response to stress situations can modulate cellular processes involved in all stages of carcinogenesis. In this work, we used breast cancer cell lines (MCF-7 and MDA-MB-231) to evaluate mRNA levels of ATP2A2 and ATP2A3 genes in response to RSV treatment. Our results demonstrate that RSV treatment induced the expression of ATP2A3 gene in both cell lines in a time and concentration-dependent manner, while the expression of ATP2A2 gene remained unaffected. The RSV-induced expression of SERCA3 in these breast cancer cell lines produced decreased cell viability, triggered apoptosis and changes in cytosolic Ca levels, as well as changes in the capacity for Ca release by the ER. These data suggest an important participation of SERCA3 genes in RSV-mediated anti-tumor effect in breast cancer cell lines. Nevertheless, further research is needed to elucidate the molecular mechanisms underlying this effect.

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Phytoestrogens induce differential effects on both normal and malignant human breast cellsin vitro

Abstract: This study demonstrates that phytoestrogens have antiproliferative effects on breast cancer cells via an ER-dependent mechanism, even at low concentrations, but are also capable of maintaining the survival of normal breast cell via ER-independent or other mechanisms.

Cited by 31 publications

References 27 publications

Effects of phytoestrogens on the activity and growth of primary breast cancer cells ex vivo

Effects of phytoestrogens on the activity and growth of primary breast cancer cells ex vivo

Impact of lower concentrations of phytoestrogens on the effects of estradiol in breast cancer cells

ATP2A3 gene as an important player for resveratrol anticancer activity in breast cancer cells

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