Phytoestrogens<i>β</i>-Sitosterol and Genistein Have Limited Effects on Reproductive Endpoints in a Female Fish,<i>Betta splendens</i>

Brown, Alexandria C.; Stevenson, Louise; Leonard, Heather M.; Nieves-Puigdoller, Katherine; Clotfelter, Ethan D.

doi:10.1155/2014/681396

Cited by 20 publications

(18 citation statements)

References 50 publications

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“…Similarly, the total intake of isoflavones did not result in difference in the GSI of trouts and these values were significantly correlated with plasma VTG densitometry. This result is in agreement with data on Siamese fighting fish ( Betta splendens ) exposed to genistein (Brown, Stevenson, Leonard, Nieves‐Puigdoller & Clotfelter, ) and results obtained by a study on striped bass ( Morone saxatilis ) in which fish treated with genistein had GSI values similar to those of controls (Pollack et al., ). However, genistein and daidzein taken in with the diet had dose‐ and time‐related effects on gonad growth (GSI) in male and female of goldfish ( Carassius auratus ) (Bagheri, Imanpoor & Jafari, ).…”

Section: Discussionsupporting

confidence: 91%

Effects of dietary soy isoflavones on estrogenic activity, cortisol level, health and growth in rainbow trout, Oncorhynchus mykiss

et al. 2018

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Soy isoflavones (the phytoestrogens genistein, daidzein and glycitein) may act as estrogen receptor agonists or antagonists. The aim of this study was to examine the effect of dietary isoflavones on growth, reproduction and health in rainbow trout (Oncorhynchus mykiss). Rainbow trout fed three experimental diets containing different concentrations of isoflavones (0, 500 and 1,500 ppm). Growth, estrogenic activity, plasma estradiol levels, gonadal development, state of stress and histological changes in selected tissues were evaluated at the end of 70 days. Neither growth performance nor the relative mRNA levels of Insulin Growth Factor I (igf‐I) in the liver were influenced by different levels of dietary isoflavones. Plasma and liver vitellogenin (VTG) protein levels and plasma 17‐β‐estradiol (E2) were unaffected by treatments, although the correlation between plasma levels of E2 and VTG densitometry values was significant (p < .05). The fish gonadosomatic index (GSI) did not significantly differ among the three experimental groups but correlated with plasma VTG densitometry values (p < .05). Plasma, muscle and fin cortisol concentrations fell within the normal welfare range and were not correlated with isoflavone levels. Histologically, the distal intestine showed a normal morphology with well‐differentiated enterocytes and in the liver hepatocytes were also normal. A supranuclear accumulation of lipid droplets in enterocytes and some lipid droplets in hepatocytes were observed in all tested groups, suggesting an impact of basal dietary lipid on transport/metabolism of fat in the fish. Overall, the present results suggest that the doses of isoflavones tested do not compromise rainbow trout reproduction, growth and health.

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Section: Discussionsupporting

confidence: 91%

Effects of dietary soy isoflavones on estrogenic activity, cortisol level, health and growth in rainbow trout, Oncorhynchus mykiss

et al. 2018

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“…Recent years have seen increased interest in the human health benefits of phytoestrogen consumption, particularly in the treatment of hormone dependent cancers, menopausal symptoms, and cardiovascular disease (Adlercreutz, 1995;Setchell and Cassidy, 1999;Beck et al, 2005). The three classes of phytoestrogens (isoflavones, coumestans, and lignans) are found at high concentrations in plants such as legumes (Dixon, 2004) and fennel (Choi and Hwang, 2004). While terrestrial animals are exposed to phytoestrogens primarily through herbivory (Bennetts et al, 1946;de Man and Peeke, 1982), aquatic animals are exposed to waterborne phytoestrogens from several sources (Clotfelter and Rodriguez, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…While terrestrial animals are exposed to phytoestrogens primarily through herbivory (Bennetts et al, 1946;de Man and Peeke, 1982), aquatic animals are exposed to waterborne phytoestrogens from several sources (Clotfelter and Rodriguez, 2006). Fennel Foeniculum vulgar extracts proved to have antiinflammatory, antispasmodic, carminative, diuretic, expectorant, laxative, analgesic, stimulant of gastrointestinal mobility and are used in treatment of nervous disturbances (Choi and Hwang, 2004). Moreover, the effect of fennel extract on reproductive system and tissues of male rats has been investigated (Torkezahrani et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Nazari¹,

Roozbehani²

2015

Turk. J. Fish. Aquat. Sci.

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Ornamental fish breeding and growing are an important sector of aquaculture, which has increased income and occupations in the country in recent years. Presenting the new methods and producing the new food supplements could have effects on efficiency and sustainable development in this new technology. In the present study, the effect of ethanolic extract of F. vulgar having phytoestrogenic compounds on growth performance and fertility rate and gonadal tissue changes of guppy was tested for offering it as a food supplement in the aquaculture. 360 juvenile guppies were distributed into 12 glass aquaria at 30 fish/ tank in triplicate. Four diets containing varying levels of alcoholic extract of F. vulgar seed at 0. 75, 100, 125 µl/g were prepared and fed to the fish twice daily at 3% of their body weight for 133 days. Biometry of the groups was done every 15days. The born fry were separated from the mothers and counted. One fish from all groups were fixed in formalin buffered 10% every 15days for histology. 5 microns sections were prepared and stained with H&E technique. The results for each group were compared statistically with those for the control. Analysis of the results showed a significant increase in weight gain and fertility rate in group T2 (100 µl/g). Gonad cells increased in the test groups comparing with control group simultaneously with raise in phytostrogenes in tests groups. In the follicles, enlarge granulose layer with increase in number of ovules in different evolution stage (O 1 -O 7 ), were seen in test groups. The increase in fertility rate in tests groups may be due to the increase in the level of estrogenic compound which in turn could be due to the action of the extract present in the diet. The observation made in the present investigation show that F. vulgar can act as a phytoestrogenic compound at low concentration and hence F. vulgar can be considered as a potential as well as a natural additive to fish feed in commercial aquaculture to augment the fertility rate.

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“…Further investigations in zebra fish confirm an estrogenic activity of genistein [19]. Other investigations point only to limited effects of genistein on reproductive parameters and hormone levels in fish [20][21][22].…”

Section: -14]mentioning

confidence: 97%

Case study with natural substances on the various options to identify and categorise endocrine disruptors

Schuhmacher-Wolz¹,

Voss²,

Schneider³

2017

J Toxicol Health

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Background: Substances with endocrine disrupting properties are under special consideration in European Union regulations. In 2014 the European Commission published a roadmap which presented four possible options for identifying endocrine disruptors (ED). A further option was proposed by the chemical and crop protection industry. Methods: This case study investigated how natural substances (genistein, caffeine, vitamin D3, sucrose) would possibly be classified under these different options when the focus is on EATS (estrogenic, androgenic, thyroid and steroidogenic) mediated effects on basis of published data. Results: For sucrose no evidence for endocrine disrupting activity could be identified. The phytoestrogen genistein as well as caffeine would possibly be classified as ED, but caffeine only if potency is not regarded. Vitamin D3 does not elicit EATS mediated adverse effects in vivo. Conclusion: This screening approach reveals that substances for which safe use in humans is known from decades of experience might possibly be classified as endocrine disruptors with yet unknown regulatory consequences. The examples show that additional criteria beyond the WHO/IPCS definition for ED might be critical for the final classification of a substance as ED.

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Phytoestrogensβ-Sitosterol and Genistein Have Limited Effects on Reproductive Endpoints in a Female Fish,Betta splendens

Cited by 20 publications

References 50 publications

Effects of dietary soy isoflavones on estrogenic activity, cortisol level, health and growth in rainbow trout, Oncorhynchus mykiss

Effects of dietary soy isoflavones on estrogenic activity, cortisol level, health and growth in rainbow trout, Oncorhynchus mykiss

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Case study with natural substances on the various options to identify and categorise endocrine disruptors

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