Phytoecdysteroids from five species of the genus Silene

Ramazonov, Nurmurod Sheralievich; Mamadalieva, Nilufar Z.; Bobaev, I. D.

doi:10.1007/s10600-007-0049-6

Cited by 7 publications

(3 citation statements)

References 4 publications

(4 reference statements)

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“…The comparison of the obtained data and spectral characteristics [Ramazanov 2007b] with data from literature has allowed to identify the ecdysteroid. +94.2±2 ° (with 0.50; methanol).…”

Section: -Hydroxyecdysonementioning

confidence: 99%

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Phytoecdysteroids-containing extract from Stachys hissarica plant and its wound-healing activity

Ramazanov

Bobayev

Yusupova

et al. 2016

Natural Product Research

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A number of phytoecdysteroid compounds, such as ecdysterone, polipodin V, 2-deoxy-20-hydroxyecdysone, integristeron A and 2-deoxydizon were isolated from Stachys hissarica plant and their structures were confirmed by NMR, mass and IR spectroscopy. In addition, the biological activity of the S. hissarica plant's extract was tested on rats for wound healing activity. It was shown that the extract at repeated oral (per os) administration at a dose of 10 mg/kg speeds up the healing process of linear skin wounds in rats. The wound-healing activity of S. hissarica extract is confirmed to be effective and exceeds known drug methyluracil (2,4-dioxo-6-methyl-1,2,3,4-tetrahydropyrimidine), especially in case of alloxan induced diabetic animals.

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“…The comparison of the obtained data and spectral characteristics [Ramazanov 2007b] with data from literature has allowed to identify the ecdysteroid. +94.2±2 ° (with 0.50; methanol).…”

Section: -Hydroxyecdysonementioning

confidence: 99%

“…(5), 316 (10), 99 (12), 81 (13), 69 (15). The structure of compound 2 was confirmed by spectral data and by comparison with original sample on thin layer chromatography [Ramazanov 2007b]. (3 The spectral data and comparison with the original sample (by TLC) have allowed to identify the integristeron A [Shishkin 1963].…”

Section: Polipodin B (2)mentioning

confidence: 99%

Phytoecdysteroids-containing extract from Stachys hissarica plant and its wound-healing activity

Ramazanov

Bobayev

Yusupova

et al. 2016

Natural Product Research

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“…The concentration of phytoecdysteroids in plants is higher than that in insects (Qian et al 2015). β-Ecdysterone (β-EC) is a type of phytoecdysteroids commonly found in most of the plants (Mamadalieva et al 2003;Shoeb et al 2006;Coll et al 2007;Ramazonov et al 2017;Snogan et al 2007).…”

Section: Introductionmentioning

confidence: 99%

β-Ecdysterone accumulation and regulation in Ajuga multiflora Bunge suspension culture

2018

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Bunge cells contain β-ecdysterone (β-EC) that regulates the molting process of insect larvae. In this study, different conditions of culture have been studied to optimize the production of β-EC. Bunge growth fitted the curve of logistic equation with one growth cycle of 17 days. The electric conductivity of medium had a negative correlation with not only the weight of dry cell but also the β-EC accumulation, and thus, could be used for monitoring the peak of both cell growth and β-EC accumulation. The pH value of the culture medium varied from 4.67 to 5.84 and reached the maximum at the end of the culture (on the 17th day). The relation of cell growth and nutrient consumption in Bunge cell suspension culture was distinctly correlated. Continuous subculture caused a reduction in β-EC synthesis; passages 7-15, the β-EC content declined ( < 0.05). At passage 11, the β-EC content was only 42.72% of that at passage 5. Additives such as mevalonic acid (MVA), l-phenylalanine (l-Phe), α-pinene, terpineol, and nitric oxide (NO) in the suspension culture medium, could significantly promote the cell growth and stimulate β-EC accumulation. The optimal concentrations of l-Phe, MVA, terpineol, and α-pinene were 0.2 mmol/l, 10 mg/l, 1 mmol/l and 6 mmol/l, respectively, with the β-EC concentrations as 1.914 ± 0.1948 mg/g ( < 0.01), 6.012 ± 0.4252 mg/g ( < 0.01), 5.147 ± 0.4819 mg/g ( < 0.01), 2.801 ± 0.1253 mg/g ( < 0.01), respectively. The optimal concentration of sodium nitroprusside, the provider of NO, was 3 mmol/l with the β-EC concentration 2.87 ± 0.2493 mg/g ( < 0.01). The results offer a strategy for massive production of β-EC.

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