Phytoecdysteroids-containing extract from Stachys hissarica plant and its wound-healing activity

Abstract

A number of phytoecdysteroid compounds, such as ecdysterone, polipodin V, 2-deoxy-20-hydroxyecdysone, integristeron A and 2-deoxydizon were isolated from Stachys hissarica plant and their structures were confirmed by NMR, mass and IR spectroscopy. In addition, the biological activity of the S. hissarica plant's extract was tested on rats for wound healing activity. It was shown that the extract at repeated oral (per os) administration at a dose of 10 mg/kg speeds up the healing process of linear skin wounds in rats. The wound-healing activity of S. hissarica extract is confirmed to be effective and exceeds known drug methyluracil (2,4-dioxo-6-methyl-1,2,3,4-tetrahydropyrimidine), especially in case of alloxan induced diabetic animals.

Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)‐SCID mice with humanized liver, human urine samples, and estimation of prevalence of its use in anti‐doping samples

“…The breakdown of ecdysterone differs considerably between species, generating different metabolites. For example, in human urine excretion of deoxy‐ecdysterone metabolites were reported as 2‐deoxyecdysterone, deoxyecdysone and 14‐deoxy‐ecdysterone; 10,11 in calf urine, 14‐deoxy‐20‐hydroxyecdysone, 20,26‐dihydroxyecdysone and 14‐deoxy‐20,26‐dihydroxyecdysone; 12 and in mice/rat excretion: 14‐deoxy‐20‐hydroxyecdysone, poststerone and 14‐deoxypoststerone 7,13 . The inclusion of ecdysteroids on WADA monitoring list has regenerated interest in investigating its metabolism and prevalence of use in humans.…”

Genus Stachys: A Review of Traditional Uses, Phytochemistry and Bioactivity

“…Various non-volatile chemical constituents have been reported from different species of genus Stachys , categorizing into important chemical groups including fatty acids, alkaloids (e.g., stachydrine, turiaine), triterpenes, phytosterols, phytoecdysteroids, diterpenes, iridoids, flavonoids, phenylpropanoid glucosides, acetophenones, phenylethanoid glycosides, lignans, phenolic acids, megastigmanes and polysaccharides [ 3 , 20 , 21 , 23 , 67 ]. The present survey was focused on all the above groups, excluded fatty acids and alkaloids due to the limited available studies.…”

“…include phytoecdysteroids ( Table 14 and Table 28 ). Ramazanov and co-workers (2016) isolated five phytoecdysteroids from S. hissarica [ 67 ], namely 20-hydroxyecdysone ( 239 ), polipodin B ( 240 ), integristeron A ( 241 ), 2-desoxy-20-hydroxyecdysone ( 242 ) and 2-desoxyecdyson ( 243 ).…”

“…In the same study, the n -butanol extract exerted antinociceptive effect at dose-dependent manner. Ramazanov et al (2016) evaluated the wound healing activity of the extract of S. hissarica on rats, showing that the extract improved the healing process of linear skin wounds at an oral dose of 10 mg/kg [ 67 ]. Of note, the wound healing activity of the extract was more effective than the known drug methyluracil (2,4-dioxo-6-methyl-1,2,3,4- tetrahydropyrimidine), especially in case of alloxan induced diabetic animals.…”

Separation and HPLC Characterization of Active Natural Steroids in a Standardized Extract from the Serratula coronata Herb with Antiseborrheic Dermatitis Activity

“…In light of the above, there is an increasing interest in phytoecdysones—natural steroids found in plants—which are seen as an alternative treatment and/or complementary skin care treatment for SD [ 26 ]. However, their immunological significance and possible therapeutic use in SD still remain unclear.…”