Phytoconstituents of leaf extracts of Ziziphus jujuba Mill. plants harvested in Tunisia

Abstract

The present study aimed to determine the phytoconstituent compositions of the leaves of four Ziziphus jujuba ecotypes (Choutrana, Mahdia, Mahres and Sfax). The chromatographie peaks of 18 compounds, including nine major fatty acids, live sterols, two triterpene alcohols and two methysterols, were quan tified by the capillary gaseous chromatography method. The major fatty acids identified were linolenic (42.04%) and palmitic (23.04%). Unsaturated fatty acids ranged between 53% and 60%. The predominant sterols (mg/100 g) were 13-sitosterol (4036) and stigmasterol (24. 18). Cycloartenol (68.55 mg/100 g) and citrostadienol (12.27 mg/lOOg) were the major metylsterols. Methylene cydoartanol ranged between 1.2 mg/100 g (Sfax) and 1.5 mg/100 g (Mahdia). Total phenolic content measured by Folin-ciocalteux ranged from 3.97 mg GAE/g to 6.04 mg GAE/g. The predominant flavonoids identified by HPLC were apigenin (6.1 mg/g) and rutin (1.91 mg/g). The fatty acids and flavonoids in the Z. jujuba leaves were responsible for their therapeutic and phar maceutical effects. This could explain whyTunisian people traditionally use it as medicine to treat several pathologies.

Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis

“…The extraction method described previously [23, 24] has been used with a slight modification. Briefly, 25 g of the powder of each plant was macerated in 250 ml of methanol under agitation of 500 rpm at room temperature for 6 h. The resulting mixture was filtered using Whatman filter paper n°1 and then evaporated under vacuum.…”

In Vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: A Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus

“…However, our findings in a previous study show that aqueous extracts have not shown any antibacterial activity due to the influence of solvents nature and polarity. For this reason, ethanol extracts of the seventeen most common plants cited by the healers (FI≥8.88%) have been prepared in accordance to the methods described by Elaloui et al and Yeo et al with slight modifications [17,18]. The used part of each plant as mentioned by herbalists was grounded to powder, and then 10 g of the powder of each plant was macerated in 100 ml of ethanol under agitation (500 rpm), at room temperature for 6 h. The resulting mixture was filtered through Whatman filter n°1 then evaporated under vacuum.…”

Protective Effect of Bioactivity Guided Fractions of Ziziphus jujuba Mill. Root Bark against Hepatic Injury and Chronic Inflammation via Inhibiting Inflammatory Markers and Oxidative Stress

“…Ziziphus jujuba Mill. (Rhamnaceae) is one such plant used by traditional healers for the treatment of liver ailments, cancer, diabetic complications, insomnia, anemia, diarrhea, and loss of appetite ( Elaloui et al, 2016 ). This plant possesses different chemical components like alkaloids, flavonoids, glycosides, and phenolic group of compounds ( Yoshikawa et al, 1997 ; Pawlowska et al, 2000 ).…”