Phytoconstituents from Gelidiella acerosa induce apoptosis by regulating Bax, Bcl2 expression in A549 cells

Begum, Sajeli; Hemalatha, S.

doi:10.1016/j.bcab.2020.101757

Cited by 5 publications

(9 citation statements)

References 17 publications

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“…Furthermore, the upregulation of antiin ammatory marker con rms the anti-in ammatory potential of the algal compounds. Our previous studies [21,22] has revealed the anticancer and antimetastatic properties of the algal extract and this current investigation has revealed the anti-in ammatory property of the extract both underin silicoand in vitro conditions. As in ammation can cause cancer progression, then the inhibition of in ammation must in turn inhibit tumorigenesis.…”

Section: Discussionmentioning

confidence: 57%

“…It was authenticated and a voucher specimen was deposited at CMFRI, Mandapam, Tamil Nadu, India (Accession No: MMR-CMFRI17002). The seaweed was extracted as previously described [22] and the ethyl acetate extract (GAE), was investigated for anti-in ammatory activity. The structures of the algal compounds analyzedby GC-MS were retrieved from PubChem and subjected to in silico analysis.…”

Section: Seaweed Collection Extraction and Characterizationmentioning

confidence: 99%

“…The marine red algae are a rich in phytocompounds with varied bioactivities. In an earlier study, [21,22] we analyzed the anticancer and antimetastatic activities of marine red algae Gelidiella acerosa under both in vitro and in vivo conditions. The current study now analyzed the interaction of isolated algal compounds with IkBα-NFKB-p65-p50 complex under in silico, to determine the e cacy of the algal extract on SOD and POX activities in adenocarcinoma cell line A549, to analyze the expression of NFKB, proin ammatory cytokines (TNF α and IL-1β) and anti-in ammatory cytokine IL 10 under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

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Gelidiella Acerosacompounds Target NFKB Cascade in Lung Adenocarcinoma

Begum

Hemalatha

2021

Preprint

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In carcinogenesis, increased metabolism, abnormal functioning of mitochondria, peroxisomes, aberrant cell signaling and prolonged inflammation can result in the overproduction of reactive oxygen species (ROS). In turn, excess ROS can upregulate the expression of various signaling pathways including the MAP kinase, PI3K/Akt and NFKB cascades in cancer. The constitutive expression of NFKB causes drug resistance in lung cancer. Hence, drugs that can enhance the antioxidant activity of enzymes and regulate the NFKB activity are of prime target to manage the drug resistance and inflammation in cancer. This study evaluated the effect of compounds present in ethyl acetate extract of Gelidiella acerosa on inflammation and on antioxidant enzymes in lung cancer. The anti-inflammatory activity was determined under in silicoandin vitro conditions. The in silico analysis showed that the phyto-constituents of G.acerosainhibit the IKBα-NFKB-p65-p50 complex in a similar way as that of doxorubicin and dexamethasone.Similarly,G.acerosatreatment enhanced the efficiency of antioxidant enzymes peroxidases and superoxide dismutase in A549 lung cancer cells. Further, the results of in vitro analysis showed that G.acerosa can inhibit the activation of NFKB, production of proinflammatory cytokines and upregulate the expression of IL 10. As inflammation causes cancer progression, the inhibition of inflammation inhibits tumorigenesis. Hence, based on the results of the study, it can be concluded that G.acerosa exerts anti- inflammatory activity through inhibition of NFKB cascade and moreover,the phyto-constituents ofG.acerosa may have the potential to treat cancer successfully.

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Section: Discussionmentioning

confidence: 57%

Section: Seaweed Collection Extraction and Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gelidiella Acerosacompounds Target NFKB Cascade in Lung Adenocarcinoma

Begum

Hemalatha

2021

Preprint

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“…The mRNA expressions of CDK2, Bcl2 and Bax were investigated by qPCR to further study the effect of DOX@Fe 3 O 4 -JMPs on cells. CDK2 is associated with the G1/S, S-phase entry and chromosomal replication, while pro-apoptosis protein Bax and anti-apoptosis protein Bcl-2 are biomarkers of cell apoptosis. , In order to ensure that the number of cells was enough to extract total RNA, A549 cells were treated with DOX@Fe 3 O 4 -JMPs only for 24 h. As shown in Figure D, the CDK2, Bcl2, and Bax mRNA expressions of A549 cells treated with JMPs showed no significant difference compared to those of the control group. However, the CDK2 of A549 cells treated with DOX@Fe 3 O 4 -JMPs was significantly down-regulated in comparison with the JMPs and control groups, suggesting that the cell cycle of cells arrested in the G1 phase, and DOX released by DOX@Fe 3 O 4 -JMPs made the cell proliferation stagnate.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Red Blood Cell-Mimicking Janus Microparticles with Enhanced Upconversion Luminescence and Drug Delivery Performance Using Double-Needle Electrospray

Cui

et al. 2023

ACS Appl. Polym. Mater.

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The shape and function of micro-/nano-particles for loading drugs play an important role in the transportation process of the drug delivery system. This study successfully fabricated red blood cell (RBC)-mimicking Janus microparticles (JMPs) using doubleneedle electrospray, which contained porous Fe 3 O 4 magnetic nanoparticles (MNPs) and upconversion (UC) luminescent nanoparticles NaYF 4 :Yb 3+ ,Er 3+ and exhibited a unique two-compartment structure. The JMPs possessed excellent magnetic and UC luminescent properties, as well as good hemocompatibility and cellular compatibility. Importantly, the two compartments of the Janus structure significantly enhanced the UC luminescent intensity compared to single compartment microparticles by reducing direct interactions between Fe 3 O 4 MNPs and UC luminescent materials. By utilizing the porous structure of Fe 3 O 4 MNPs, doxorubicin hydrochloride was loaded into Fe 3 O 4 MNPs and then embedded into the JMPs resulting in a slow-release effect. Given the excellent properties of magnetic and UC luminescence, biocompatibility, and drug slow-release ability, the RBC-mimicking JMPs have significant potential for drug delivery and bioimaging applications.

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“…Inhibition is regulated by the PI3K/AKT pathway, whereas EG treatment reduced the activity of substrate metalloproteinase 2 (MMP2) and MMP9 in MDAMB231 cells [72]. Apoptotic induction is regulated by changing the Bax/Bcl2 ratio [73]. GA has been shown to induce anti-proliferative and apoptotic activity in MCF-7 cells by increasing p27 levels, decreasing proliferation, and inducing cell cycle arrest in the G2/M phase [74].…”

Section: Anticancermentioning

confidence: 99%

The Evolution of Pharmacological Activities Bouea macrophylla Griffith In Vivo and In Vitro Study: A Review

2022

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Bouea macrophylla Griffith (B. macrophylla) is one of the many herbal plants found in Asia, and its fruit is plum mango. This plant is rich in secondary metabolites, including flavonoids, tannins, polyphenolic compounds, and many others. Due to its bioactive components, plum mango has powerful antioxidants that have therapeutic benefits for many common ailments, including cardiovascular disease, diabetes, and cancer. This review describes the evolution of plum mango’s phytochemical properties and pharmacological activities including in vitro and in vivo studies. The pharmacological activities of B. macrophylla Griffith reviewed in this article are antioxidant, anticancer, antihyperglycemic, antimicrobial, and antiphotoaging. Each of these pharmacological activities described and studied the possible cellular and molecular mechanisms of action. Interestingly, plum mango seeds show good pharmacological activity where the seed is the part of the plant that is a waste product. This can be an advantage because of its economic value as a herbal medicine. Overall, the findings described in this review aim to allow this plant to be explored and utilized more widely, especially as a new drug discovery.

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Phytoconstituents from Gelidiella acerosa induce apoptosis by regulating Bax, Bcl2 expression in A549 cells

Cited by 5 publications

References 17 publications

Gelidiella Acerosacompounds Target NFKB Cascade in Lung Adenocarcinoma

Gelidiella Acerosacompounds Target NFKB Cascade in Lung Adenocarcinoma

Fabrication of Red Blood Cell-Mimicking Janus Microparticles with Enhanced Upconversion Luminescence and Drug Delivery Performance Using Double-Needle Electrospray

The Evolution of Pharmacological Activities Bouea macrophylla Griffith In Vivo and In Vitro Study: A Review

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