Phytochrome from green Avena shoots characterized with a monoclonal antibody to phytochrome from etiolated Pisum shoots

The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome.

“…It has similarly been reported that the most abundant type 2 phytochrome extracted from lightgrown tissue exhibits a slightly lower apparent molecular mass than type 1 phytochrome: 6 kD lower both in oat (Tokuhisa and Quail, 1989) and pea, in which it shows homology to predicted PHYB (Abe et al, 1989). Nevertheless, apparent molecular sizes of phytochromes have been shown to vary according to experimental conditions (Cordonnier et al, 1986), and, in oats, for example, it is possible that two 124-and 118-kD species (Tokuhisa and Quail, 1989) are equivalent to two 125-and 123-kD species .…”

Genetic Regulation of Development in Sorghum bicolor

“…Pea-25, which previously has been shown to readily detect one of the less abundant phytochromes that predominate in GO leaves (7,28), immunostains the 123-kD polypeptide as before (6). Also, a monoclonal antibody (GO-7) directed toward and specific for one of the phytochromes that predominates in GO leaves (123-kD oat phytochrome; ref.…”

“…Protein was electrotransferred to nitrocellulose and immunostained as before (28) with monoclonal antibodies Pea-25, Oat-16, or GO-7. Pea-25 is an antibody directed to phytochrome from etiolated pea shoots (7,8). Oat-16 and GO-7 were raised against phytochromes from etiolated and GO shoots, respectively (9,22,28).…”

Phycocyanobilin Is the Natural Precursor of the Phytochrome Chromophore in the Green Alga Mesotaenium caldariorum

“…One of these is the phycobiliprotein chromophore precursor, (3E)-phycocyanobilin (PCB), assembly of which with apophytochrome A yields a photoactive species with blue-shifted absorption maxima for both Pr and Pfr forms (11). Second, phytochromes with blueshifted absorption spectra have been reported for the fern Adiantum capillus-veneris (12), the mosses Ceratodon purpureus (13) and Atrichum undulatum (14), light-grown higher plants (15,16), and the green alga Mesotaenium caldariorum (17,18). With the notable exception of green algal phytochrome, these blue shifts were restricted to the Pfr forms, a result that can readily be ascribed to limited proteolysis or denaturation of phytochrome during its purification (19).…”