Genetic Regulation of Development in <i>Sorghum bicolor</i>

Childs, Kevin L.; Cordonnier‐Pratt, Marie‐Michèle; Pratt, Lee H.; Morgan, Page W.

doi:10.1104/pp.99.2.765

Cited by 65 publications

(21 citation statements)

References 25 publications

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“…Recently, this mu-tant was shown to be deficient in a low-abundance polypeptide that is immunostainable with both a monoclonal antibody raised against pea phytochrome and directed against a highly conserved epitope (6) and a monoclonal antibody raised against phytochrome from light-grown oat shoots. (5).…”

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confidence: 99%

Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa

Devlin

Rood²,

Somers³

et al. 1992

Plant Physiol.

116

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Several phytochrome-controlled processes have been examined in etiolated and light-grown seedlings of a normal genotype and the elongated internode (ein/ein) mutant of rapid-cycling Brassica rapa. Although etiolated ein seedlings displayed normal sensitivity to prolonged far-red light with respect to inhibition of hypocotyl elongation, expansion of cotyledons, and synthesis of anthocyanin, they displayed reduced sensitivity to prolonged red light for all three of these deetiolation responses. In contrast to normal seedlings, light-grown ein seedlings did not show a growth promotion in response to end-of-day far-red irradiation. Additionally, whereas the first internode of light-grown normal seedlings showed a marked increase in elongation in response to reduced ratio of red to far-red light, ein seedlings showed only a small elongation response. When blots of protein extracts from etiolated and lighttreated ein and normal seedlings were probed with monoclonal antibody to phytochrome A, an immunostaining band at about 120 kD was observed for both extracts. The immunostaining intensity of this band was substantially reduced for extracts of light-treated normal and ein seedlings. A mixture of three monoclonal antibodies directed against phytochrome B from Arabidopsis thaliana immunostained a band at about 120 kD for extracts of etiolated and light-treated normal seedlings. This band was undetectable in extracts of ein seedlings. We propose that ein is a photoreceptor mutant that is deficient in a light-stable phytochrome B-like species.

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confidence: 99%

Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa

Devlin

Rood²,

Somers³

et al. 1992

Plant Physiol.

116

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“…Weller, personal communication); and transgenic Arabidopsis overexpressing PHYA or PHYB (McCormac et al, 1993). In addition, the tall, earlyflowering ma,R mutant of Sorghum bicolor shows deficiency in severa1 phy-dependent responses and also lacks one type of phy that is abundant in green tissue (Childs et al, 1992).…”

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confidence: 99%

Phytochrome, Gibberellins, and Hypocotyl Growth (A Study Using the Cucumber (Cucumis sativus L.) long hypocotyl Mutant)

et al. 1995

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The possible involvement of gibberellins (CAs) in the regulation of hypocotyl elongation by phytochrome was examined. Under white light the tal1 long hypocotyl (Ih) cucumber (Cucumis sativus L.) mutant, deficient in a type B-like phytochrome, shows an increased "responsiveness" (defined as response capability) to applied CA, (the main endogenous active CA) compared to the wild type. Supplementing far-red irradiation results in a similar increase in responsiveness in the wild type. Experiments involving application of the precursor GA, and of an inhibitor of CA, inactivation suggest that both the GA, activation and inactivation steps are phytochrome independent. Endogenous C A levels of whole seedlings were analyzed by combined gas chromatography-mass spectrometry using deuterated interna1 standards. The levels of GA, (and those of CA,,, the inactivated CA,) were lower in the //I mutant under low-irradiance fluorescent light compared with the wild type, similar to wild type under higher irradiance light during the initial hypocotyl extension phase, and higher during the phase of sustained growth, in which extension involved an increase in the number of cells in the upper region. In all cases, growth of the Ih mutant was more rapid than that of the wild type. It is proposed that CA, and phytochrome control cell elongation primarily through separate mechanisms that interact at a step close to the terminal response.

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“…This mutant is also insensitive to photoperiod (Childs et al, 1992), flowering very early in a manner similar to that of BMDR-1. This result strongly suggests that phyB is involved in the detection of photoperiod.…”

Section: Discussionmentioning

confidence: 93%

“…The increased level of spectrophotometrically detectable total phytochrome was found to be largely accounted for by an increase in phyA (Principe et al, 1992), but nothing was known about the regulation of phyB levels. The hy3 mutant in Arabidopsis , the ma 3 R mutant in sorghum (Childs et al, 1992), the ein mutant in Brassica rapa (Devlin et al, 1992), and the lh mutant in cucumber (Lopez-Juez et al, 1992) all flower early and lack a light-stable phyB protein. In Arabidopsis and sorghum it has been shown that the mutation is located in the PHYB gene Childs et al, 1997).…”

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confidence: 99%