Phytochemistry and Biological Activities of Crateva Adansonii Extracts

Objective: The objective of the study was to investigate the antioxidant property of different extracts of Lindernia crustacea (L) F. Muell and isolate flavonoid from the potent extract and characterize it. Methods: Isolation was carried out by flash chromatography using Toluene:acetic acid (4:1) as eluent. The isolated compound was characterized using spectroscopic methods. 2, 2′- diphenyl-1-picrylhydrazyl, ferric thiocyanate, thiobarbituric acid, and reducing power assay methods were followed for the antioxidant study. Results: Characterization of the isolated compound confirms it as the flavonoid. Results of the antioxidant study showed that benzene extract has the highest antioxidant activity with a less IC50 value in comparison to ethyl acetate and ethanol extracts. The isolated compound showed significant antioxidant activity when compared with aspirin. Conclusion: The results of the study suggest that L. crustacea (L) F. Muell is a source of flavonoid which has potent antioxidant activity.

Section: Discussionmentioning

confidence: 99%

Isolation and in Vitro Antioxidant Activity of Flavonoid From Lindernia Crustacea (L) F. Muell

Das¹,

Ahmed²,

Shil³

et al. 2019

“…This process takes place over and over again until all the sample was extracted from the A. barbadensis miller plant. The sample obtained from the soxhlet extraction was then stored in an airtight container at 4°C for further analysis [6].…”

Section: Soxhlet Extraction Methodsmentioning

confidence: 99%

Cytotoxic Effect of Peptide Functionalized Silver Nanoparticles Synthesized From Aloin on Breast Cancer Cell Line

Vidhya

et al. 2019

Objective: The aim of the present study is to synthesize the silver (Ag) nanoparticle using Aloin with a focus on its antibacterial, antioxidant, and anticancer activity. Methods: The silver nanoparticles were synthesized using Aloin and were determined by UV-Visible spectrum. It was further characterized by scanning electron microscope (SEM), zeta potential, and dynamic light scattering (DLS). The Fourier transform infrared analysis was also carried out for the Aloin. Results: The UV-Visible absorption spectrum of the synthesized silver nanoparticles has shown the absorption peak at 439nm which proves the formation of silver nanoparticles in the solution. The SEM analysis revealed that the Ag nanoparticles were spherical in shape. The IR spectra showed that there are 6 functional groups are present in Aloin extract. The synthesized nanoparticles are found to be highly stable with an average particle size of 130.7nm which was confirmed by zeta potential and DLS analysis. The synthesized nanoparticles had a good antibacterial and antioxidant activity. It shows a very good cytotoxic effect against breast cancer cell line. Conclusion: The present study suggests that the synthesis route is free from the requirements such as high energy, extended preparation time, and special equipments and thus can be used for large-scale synthesis in food industries for food preservation and these Ag nanoparticles can be used for its therapeutic purposes for developing a new drug against cancer.

“…The TPC of the C. papaya sample was determined spectrophotometrically according to the Folin-Ciocalteu colorimetric method with slight modifications [11,12].…”

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Phytochemical Analysis and Antioxidant Properties of Leaf Extracts of Carica Papaya

Nandini

Prasad

et al. 2020

Objective: The objective of the present study aimed at investigating the phytochemical and antioxidant properties of Carica papaya leaf extracts. Methods: As phytochemicals are biologically active compounds and a powerful group of plant chemicals, believed to stimulate the immune system along with antioxidants, the molecules which hinder oxidation of other molecules by the process of inhibiting or by generating the oxidizing chain reactions and preventing diseases. The total phenolic content (TPC) was determined by Folin-Ciocalteu method and total flavonoid contents (TFC) were determined aluminum chloride method and antioxidant by 2,2,1-diphenyl-1-picrylhydrazyl method. Results: The results of phytochemical screening revealed the presence of bioactive compounds such as alkaloid, carbohydrates, and amino acids and TPC and TFC varied among the different solvent extracts, in which methanolic extracts showed highest amount of phytochemicals and TPC and TFC and antioxidants compared to other solvents. Conclusion: The isolation and purification of specific bioactive compound may account as natural and promising medicines in exploration of new drug.