Phytochemical study, potential cytotoxic and antioxidant activities of selected food byproducts (Pomegranate peel, Rice bran, Rice straw &amp; Mulberry bark)

Ibrahim, Khaled Meselhy; Shams, Manal M.; Sherif, Noheir H; El-Sonbaty, Sawsan M

doi:10.1080/14786419.2018.1488708

Cited by 16 publications

(10 citation statements)

References 8 publications

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“…A previous published results clearly showed that rice straw extract is potential of anti-proliferation activity in vitro with a promising cytotoxic effect in low dose against cancer lines of liver (HepG2), prostate (PC3), and breast carcinoma (MCF-7) while it is of moderate effect on viable lung cell line. 11 The pronounced potential cytotoxic effects of RS is in agreement with Suffness 21 who stated that a compound with IC50 less than 100 μg/mL is a potential antitumor. That encouraged us to obtain further insights into the growth inhibitory effects of RS on solid Ehrlich tumor in female mice.…”

Section: Discussionsupporting

confidence: 84%

“…This could be explained by the fact that RS extract is capable to inhibit tumor growth, mainly because of its antioxidant activity and the antitumor compounds present in the extract. In this study LC/ MS/MS (Table 2)and previous HPLC analysis 11 revealed the presence of high content of ferulic acid, p-coumaric acid, vanillic acid, quercetin, kaempferol, apigenin and catechin, known of antitumor activity 22,23 and capable to inhibit tumor growth. Previously published studies reported that ferulic acid is of antiproliferative effect by inducing G0/G1 phase arrest and downregulated the expression of cell cycle-related proteins, CDK 2, CDK 4, CDK 6.…”

Section: Discussionsupporting

confidence: 62%

“…was based on comparing the molecular ion peak in the negative [M-H]and positive[M + H] + mode and the product ion [Ms-Ms ions] with the literature[18][19][20] and previously identified compounds 11. …”

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confidence: 99%

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Phenolic Profile and In Vivo Cytotoxic Activity of Rice Straw Extract

Ibrahim¹,

Mm²,

Nh³

et al. 2019

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Background: Previous work of our team exhibited that rice straw (RS) has antitumor activity in vitro and inhibit proliferation of liver, lung, prostate, and breast cancer human cell lines. In this work, we extended our research to screen the antitumor activity of RS ethanol extract as a single treatment and in the presence of combined radiotherapy with a low dose of gamma radiation against murine Ehrlich solid carcinoma (EAC) model. Objective: To evaluate the most common waste in Egypt RS to screen out its in vivo cytotoxic activity and as combined therapy with radiotherapy. Method: Tested sample RS was investigated for its content of phenolics by LC/MS/MS, in addition, ethanolic extracts of the tested sample were investigated as antitumor on female mice inoculated with EAC cells as a single treatment and in the presence of combined radiotherapy with a low dose of gamma radiation (LDR). Results: LC/MS/MS revealed that rice straw was rich in phenolic acids (vanillic, p-coumaric, ferulic, and sinapic acid) along with catechin and flavonoids aglycones (quercetin, apigenin, and kaempferol). Rice straw and/or exposure to a low dose of γ-radiation caused a marked suppression of tumor growth and induced significant reduction in VEGF level & in IL-6 level with significant elevation in IL-10 serum level. Rice straw caused a significant down regulation in the gene transcription level of MCL1 and b-catenin, and a significant up-regulation of Caspase-3 and Bax gene expression. RS extract and LDR (EC + RS + R group) revealed that there was a mild form of necrosis with severe apoptosis in the tumor cells. Conclusion: From the aforementioned results, it can be concluded that RS/LDR effectively and synergistically work towards inhibition of cancer cell proliferation. These findings were well supported with histopathological studies suggesting that RS/low dose gamma radiation can serve as a good therapeutic agent against cancer but still need further clinical studies.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 62%

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Phenolic Profile and In Vivo Cytotoxic Activity of Rice Straw Extract

Ibrahim¹,

Mm²,

Nh³

et al. 2019

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“…To establish uniformity in the cell viability studies, the dosage in µl/ml is used instead of the final concentration as it will be difficult to compare the cytotoxicity and viability data. The use of µl/ml has previously been used for cytotoxicity studies (Karaman et al 2017;Meselhy et al 2018;Shehzad et al 2018). The concentrations of phytosynthesized MgO nanoparticles in the sample dosages are presented in the supplementary material section (Table S.8).…”

Section: Mtt Assaymentioning

confidence: 99%

Cytotoxicity and insulin resistance reversal ability of biofunctional phytosynthesized MgO nanoparticles

2020

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The present study investigates the cytotoxicity of hexagonal MgO nanoparticles synthesized via Amaranthus tricolor leaf extract and spherical MgO nanoparticles synthesized via Amaranthus blitum and Andrographis paniculata leaf extracts. In vitro cytotoxicity analysis showed that the hexagonal MgO nanoparticles synthesized from A. tricolor extract demonstrated the least toxicity to both diabetic and non-diabetic cells at 600 μl/ml dosage. The viability of the diabetic cells (3T3-L1) after incubation with varying dosages of MgO nanoparticles was observed to be 55.3%. The viability of normal VERO cells was 86.6% and this stabilized to about 75% even after exposure to MgO nanoparticles dosage of up to 1000 μl/ml. Colorimetric glucose assay revealed that the A. tricolor extract synthesized MgO nanoparticles resulted in ~ 28% insulin resistance reversal. A reduction in the expression of GLUT4 protein at 54 KDa after MgO nanopaSrticles incubation with diabetic cells was observed via western blot analysis to confirm insulin reversal ability. Fluorescence microscopic analysis with propidium iodide and acridine orange dyes showed the release of reactive oxygen species as a possible mechanism of the cytotoxic effect of MgO nanoparticles. It was inferred that the synergistic effect of the phytochemicals and MgO nanoparticles played a significant role in delivering enhanced insulin resistance reversal capability in adipose cells.

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“…Rice bran has been widely used as a substrate to produce value-added products (Meselhy et al, 2020;Wang et al, 2020). P. sumatrense NCH-S2 was grown in a liquid culture for eight days using rice bran as the substrate.…”

Section: Optimization Of Fae Productionmentioning

confidence: 99%