Phytochemical screening of plant extracts and GC-MS analysis of the n-hexane extracts of stems and roots of Catharanthus roseus growing in Bangladesh

Abstract

Phytochemical screening and gas chromatography-mass spectrometry (GC-MS) methods were used to determine the phytochemical components of various extracts of stems and roots of Catharanthus roseus. Preliminary phytochemical screening of n-hexane, chloroform, ethyl acetate and methanol extracts of stems and roots of Catharanthus roseus were carried out to identify the class of compounds present in each extract. The results exhibited that steroids, terpenoids, flavonoids and phenolic compounds were found in every extract of stems and roots. Alkaloids were also found in all extracts except n-hexane. But carbohydrates were absent in all extracts of stems and roots except methanol. The GC-MS analysis of the n-hexane extract of the stems and roots of Catharanthus roseus was done, which allowed the identification and quantification of 30 and 22 n-hexane soluble compounds present in the stems and roots, respectively. In this experiments, D:B-Friedo-B':A'-neogammacer-5-en-3β-ol (10.23%) and Ethyl palmitate (13.63%) identified with the highest concentration in the n-hexane extract of stems and roots, respectively.

Catharanthus roseus: Alkaloid Fraction Isolation and its Antibacterial Activity

“…There is no universal method which can be applied for the extraction of secondary metabolites from Catharanthus roseus (Kumar et al, 2022). However, the phytochemical analysis reveals the presence of steroid and alkaloid in the extract (Uddin et al, 2022). Tikhomiroff and Jolicoeur ( 2002) reported low extraction yields when using a single methanol extraction step for intracellular serpentine, vincristine, vindoline, catharanthine, tabersonine and tryptophan while a relatively high yield was shown by multiple successive extraction of plant parts using different solvents (Hisiger and Jolicoeur, 2007).…”