“…There is no universal method which can be applied for the extraction of secondary metabolites from Catharanthus roseus (Kumar et al, 2022). However, the phytochemical analysis reveals the presence of steroid and alkaloid in the extract (Uddin et al, 2022). Tikhomiroff and Jolicoeur ( 2002) reported low extraction yields when using a single methanol extraction step for intracellular serpentine, vincristine, vindoline, catharanthine, tabersonine and tryptophan while a relatively high yield was shown by multiple successive extraction of plant parts using different solvents (Hisiger and Jolicoeur, 2007).…”