Phytochemical Screening, Nutrient Analysis and Antimicrobial Activity of the Leaves of Lasianthera africana and Dennettia tripetala on Clinical Isolates

Ebana, R. U. B.; Asamudo, N. U.; Etok, C. A.; Edet, U. O.; Onyebuisi, C

doi:10.9734/jabb/2016/28291

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…Anthraquinone was not present in this study, while Phlobotannins was moderately present. The report of this study was in agreement with the results of Ekanem et al (2016), Ebana et al (2016) who carried out the phytochemical screening of extract of L. africana and H. africana and reported no trace of anthraquinone and moderately presence of Phlobatannins.…”

Section: Discussionsupporting

confidence: 92%

Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais

Oboho¹

2021

IJAB

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The present study was carried out to assess the effect of methanol leaf extract of Uvaria chamae using Gas Chromatography-Mass Spectrum (GC-MS) to determine the phytochemicals present and its effect on the histology of midgut of maize weevil, Sitophilus zeamais. Insects were administered with 10 mg/kg of the plant extract using diffusion method where insects were put in a petri dish containing various concentrations and observed to see the stage they begin to die due to toxicity and observed for 5 min. They were collected into foil processing paper and fixed in Bouins fluid for 24 h, repacked after 24 h and folded in fresh foil immersed in buffered formalin for histopathological studies. Result revealed that a severe degeneration de-arrangement of the respiratory tract epithelial lining, secretory lining cells and gastrointestinal layers with the destruction of the muscular layer when compared with the control. The methanol leaf extracts of U. chamae were preliminary screened for the phytochemicals. The extract shows the presence of cardiac glycosides, saponin, steroids/terpenes, flavonoids, alkaloids and phenols. GC-MS analysis of the extract showed the presence of 2-nitrobenzaldehyde (4.00), malic acid (2.04), L-aspartic acid (2.00), 1, 1, dimethylhydrazine (1.86), Cedrandiol (1.75), 2-amino-4-(2-methylpropenyl)-pyrimidin-5-carboxylic acid (1.56), thiirane (1.54), mercaptoethanol (1.11) and some minor compounds. The findings indicated that methanol extract of U. chamae is rich in phyto-compounds having biological activities on the midguts’ histology of S. zeamais. Therefore, it is recommended as an alternative for the synthetic insecticide used by farmers for the preservation of stored grains. © 2021 Friends Science Publishers

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Section: Discussionsupporting

confidence: 92%

Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais

Oboho¹

2021

IJAB

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“…Anti-nutrient analysis showed that hydrocyanic acid, total oxalate, soluble oxalate, phytate and tannins ranged from 1.65-2.62, 14.43-35.72, 4.70-18.50, 0.09-0.83 and 0.12-0.41 mg/100 g dry matters, respectively. When compared with edible vegetables and mushroom previously examined, these were found to be within the same range and lower than world maximum allowable limits in foods [18,25,27,32,33]. Furthermore, all examined anti-nutrients were lower than those reported earlier for Citrus paradis peels [24], test and seeds of Dacryodes eludis and Garcinia kola [32], and as well as pod and seeds of Cola rostada and Cola nitida [34].…”

Section: Discussionmentioning

confidence: 69%

“…The anti-nutrients examined were hydrocyanic acid, phytic acid and oxalate. These were done as previously described [25,26,27].…”

Section: Estimation Of Anti-nutrientsmentioning

confidence: 99%

Bread Production from Different Flours Using Strains of Baker’s and Palm Wine Yeasts

Ebana

Edet

Anosike

et al. 2018

AFSJ

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Bread is widely eaten across the world and the science of its baking has come a long way. This study was aimed at evaluating the effect of using Baker's and palm wine yeasts on the performance and sensory properties of bread baked with bean (Phaseolus vulgaris), corn (Zea mays), golden penny and wheat flours in addition to nutritional analysis of the flours and Baker's yeast. Collection of samples, processing of flours, palm wine yeast isolation, proximate and elemental analyses, and baking of the various breads were done using standard methodologies. Sensory evaluation was performed using Likert scale and replicate readings were analysed using analysis of variance. Baker's yeast had the highest amount of fat, ash and protein but lower than the rest in fiber and carbohydrate content. Amongst the flours examined for proximate composition, bean flours had higher amounts of moisture, ash and fiber, but not carbohydrate and fiber. Vitamins A, total and soluble vitamin C were present in all our sampled flours and Baker's yeast.

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“…The leaf was identified by a Botanist Professor Ani Nkang and identity number AJU/2021/12 assigned. The leaves were processed as previously reported [ 9 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…For this study, two types of extracts were prepared using methods previously reported and these were aqueous (distilled water) and ethanolic (analytical grade) [ 9 , 10 ]. To 200 ml of the respective solvents, 20 g of the pulverized powder were added to obtain a ratio of 1:10.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Annona muricata extract against Staphylococcus aureus isolate and in-silico activity of bioactive compounds against Capsular protein (Cap5O)

Edet¹,

Nwaokorie

Mbim³

et al. 2022

BMC Complement Med Ther

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Background Staphylococcus aureus has prevailed against the majority of antibiotics currently in clinical use, making it a significant global public health problem. As a safer alternative, bioactive compounds have been explored. Annona muricata has been shown to possess antimicrobial activity. However, there are few reports on the molecular activity of A. muricata bioactive compounds against S. aureus. Thus, this study was aimed at evaluating the antimicrobial activity of its crude extract as well as investigating the potential of its bioactive compounds against the Cap5O capsular polysaccharides (CPS) of S. aureus via molecular docking. Methods Collection of plant leaves, preparation of extracts, anti-nutrient analysis, phytochemical screening via crude method and gas chromatography-mass spectrophotometer (GC-MS), isolation and characterization of S. aureus and the antimicrobial activity test were all done using standard protocols. Molecular docking was done using the MCULE online tool with emphasis on docking scores, toxicity, and other properties. Results Crude screening of the extracts showed the presence of polyphenols, hydroxyanthraquinones, reducing compounds, flavonoids, saponins, glycosides, alkaloids, anthraquinones, phlobatannins and tannins in different concentrations. Anti-nutrient analysis showed the presence of allowable levels of evaluated anti-nutrients. GC-MS revealed a total of twenty-nine (29) bioactive compounds, out of which only 4 (13.80%) docked without toxicity and these were bicyclo[4.1.0]heptan-2-one 6-methyl, trichloromethane, carbonic acid 2-dimethylaminoethyl propyl ester, and 1-methyl-4-phenyl-5-thioxo-1,2,4-triazolidin-3-one on either the NAD-binding or C-terminal substrate binding domain of Cap5O. Conclusion Results obtained show that Cap5O could be a potential drug target for multi-drug resistant S. aureus, however, further studies aimed at evaluating these bioactive compounds individually and in combination are highly needed.

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Phytochemical Screening, Nutrient Analysis and Antimicrobial Activity of the Leaves of Lasianthera africana and Dennettia tripetala on Clinical Isolates

Cited by 11 publications

References 7 publications

Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais

Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais

Bread Production from Different Flours Using Strains of Baker’s and Palm Wine Yeasts

Evaluation of Annona muricata extract against Staphylococcus aureus isolate and in-silico activity of bioactive compounds against Capsular protein (Cap5O)

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