Phytochemical screening, free radical scavenging and antimicrobial potential of Chromolaena odorata leaf extracts against pathogenic bacterium in wound infections– a multispectrum perspective

Vijayaraghavan, Kavitha; Rajkumar, Johanna; Ali-Seyed, Mohamed

doi:10.1016/j.bcab.2018.05.014

Cited by 33 publications

(8 citation statements)

References 46 publications

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“…Plants contain a vast array of bioactive phytochemicals that are believed to play a role in defense reactions against infections by pathogenic microorganisms (26). Among these, phenolic acids and flavonoids are known to be biologically active against tumors, viruses and other microbes and are also considered as antioxidant agents (27).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

2020

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Antibiotic resistance is the major growing threat facing the pharmacological treatment of bacterial infections. Therefore, bioprospecting the medicinal plants could provide potential sources for antimicrobial agents. Mimusops, the biggest and widely distributed plant genus of family Sapotaceae, is used in traditional medicines due to its promising pharmacological activities. This study was conducted to elucidate the antimicrobial effect of three unexplored Mimusops spp. (M. kummel, M. laurifolia and M. zeyheri). Furthermore, the mechanisms underlying such antibacterial activity were studied. The Mimusops leaf extracts revealed significant antibacterial activities against the five tested bacterial strains with a maximum inhibition zone diameter of 22.0 mm against B. subtilis compared with standard antibiotic ciprofloxacin. The minimal inhibitory and bactericidal concentration values against tested Gram-positive and Gram-negative bacterial strains ranged from 3.15-12.5 µg/ml. However, weak antifungal effect was recorded against Candida albicans with MIC value ˃25 µg/ml. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay showed that M. caffra was the best antioxidant (IC50=14.75±0.028 µg/ml), while M. laurifolia was the least one (IC50=34.22±0.014 µg/ml). The phenolics in plant leaves extracts were identified and quantified by high performance liquid chromatography (HPLC) which revealed the presence of seven phenolic acids and four flavonoids. The abundant phenolic compounds were rutin (5.216±0.067 mg/g dried wt.) and gallic acid (0.296±0.068 mg/g) followed by myricetin (0.317±0.091 mg/g) then kaempferol (0.113±0.049 mg/g) as flavonoids. The antibacterial mechanism of M. laurifolia extract, as a representative species, induces ultrastructural changes in the model bacterium Staphylococcus aureus with cell wall and plasma membrane lysis as revealed by transmission electron microscopy. Overall, Mimusops species (M. laurifolia, M. kummel and M. zeyheri) are promising natural alternative sources for antimicrobial agents.

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Section: Discussionmentioning

confidence: 99%

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

2020

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“…Another species, Physalisperuviana, also reported possessing considerable H 2 O 2 scavenging activity. In general, the plant which possesses an elevated quantity of essential phytochemicals and bioactive compounds derived from such phytochemicals can considerably act as free radical scavenging agents [35]. The bioactive compounds, which possess considerable free radical scavenging potential can be considered as suitable agent or precursor molecule to prepare antioxidant drugs [36].…”

Section: H 2 O 2 Scavenging Potentialmentioning

confidence: 99%

Structural Prediction of Bioactive Compound Elucidated Through 1 H and 13C NMR Based Metabolomics from Physalis Minima

Pradeepkumar¹,

Muthukrishnan²,

Manikandan³

et al. 2022

J Adv Mater Sci Eng

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This study was performed to extract, purify, and characterize the bioactive compound from the ethanol extract of Physalis minima fruit. Furthermore, the free radicals scavenging potential of that purified and characterized test compound was investigated through in-vitro antioxidant studies. The study's findings demonstrated that the ethanol extract of Physalis minima fruits is supposed to contain many critical phytochemical constituents. The predominant fraction acquired from the extract via column chromatography and TLC was characterized using 13C NMR and1 H NMR techniques. The analyses results revealed that the chemical structure and name of the bioactive compound are 2-(3,4-dihydroxyphenyl) - 3, 4,4a 5-dihydro-2H-chromene-3,5,7-triol.

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“…Therefore, given the high prevalence of chronic and erosive diseases, it necessitates using plants to provide the antioxidants needed by the body, especially plants that are high in phenol and flavonoids. Therefore, to provide the natural antioxidants needed by the body, the consumption of plants with high phenolic compounds is recommended (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

et al. 2021

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Background: Because many plant extracts have a significant inhibitory effect on pathogenic microorganisms, so we aimed to evaluate the antioxidant and antimicrobial activity of some plant extracts on Bacillus cereus isolated from soil. Methods: The chicory (Cichorium intybus L.), hyacinth (Hypericum perforatum L.), lavender (Lavandula angustifolia), yew (Taxus baccata), and thyme (Thymus vulgaris L.) plants were collected and identified in the botanical laboratory of the University of Zabol. To prepare the ethanolic extract, 40 g of dried leaves of plants were used in 400 cc of ethanol. Different strains of B. cereus used in this study were isolated from soil and identified by biochemical, bacteriological, and growth tests as well as standard tests. Antimicrobial effects were investigated by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to Bauer and Kirby instructions as well as microdilution. Statistix ver10 software was used for statistical calculations. Mean comparisons were performed using the LSD at the 1% level, and Excel was also used to draw the figures. Results: The diameter of the growth inhibition zone of plant extracts against B. cereus at a dilution of 100 ppm was investigated, and it was found that different extracts had different effects on inhibiting the growth of B. cereus (P < 0.01). LSD test showed that thyme (T. vulgaris L.) had the highest (15 mm) effect on growth inhibition of strains 2 and 3 of B. cereus and the lowest (1 mm) effect on growth inhibition of strain 1 of B. cereus. The lowest MIC and MBC of thyme (T. vulgaris L.) against B. cereus samples were 3.1 and 6.2 ppm, respectively. Conclusions: Considering the side effects of chemical drugs and antibiotics, as well as the potential effect of medicinal plant extracts, especially T. vulgaris L. on B. cereus, it is recommended that T. vulgaris L. may inhibit the growth of B. cereus.

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Phytochemical screening, free radical scavenging and antimicrobial potential of Chromolaena odorata leaf extracts against pathogenic bacterium in wound infections– a multispectrum perspective

Cited by 33 publications

References 46 publications

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

Structural Prediction of Bioactive Compound Elucidated Through 1 H and 13C NMR Based Metabolomics from Physalis Minima

Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

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