Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus

Abstract

Staphylococcus aureus (S. aureus) is a pathogenic microbe that is caused by various diseases in humans and animals. Infectious diseases caused by S. aureus in Asia reached 70% in 2007, while in Indonesia reached 23.5%. The plant provided several bioactive compounds that might function as an antibacterial which inhibits both bacterial growth and damaging the cell system and protein synthesis. Coleus arthropurpureus known contains alkaloids and tannins that supposed to be an antibacterial compound. Tannins have antibacterial activity, in general, the mechanism is to damage the bacterial cell membrane and induce the formation of complex compound bonds to enzymes or microbial substrates. This study aims to analyze the bioactive compounds contained in C. arthropurpureus in qualitative and quantitative which have an antimicrobial function using high-performance liquid chromatography (HPLC) in the reverse phase C-18 column and screening of antibacterial activity was carried out by disc diffusion method. The results of both qualitative and quantitative analysis by HPLC has obtained the presence of tannin bioactive compounds (1.48 ppm at a retention time of 2.806 minutes) and alkaloids (1.11 ppm at a retention time of 7.015). Moreover, we verified the diameter of inhibition of growth zone against S. aureus at a concentration of 15% extract was 12.80 mm. It was found that the highest percentage of the bioactive compound in C. arthropurpureus is tannin, and that is might an antibacterial agent.

Antidiabetic Activity of Sungkai (Peronema canescens Jack) Leaves Ethanol Extract on the Male Mice Induced Alloxan Monohydrate

“…19 Likewise, saponins are able to reduce glucose absorption in the intestine by damaging the arrangement of cell membranes. 20,21 Unfortunately, the research on P. canescens is still minimal, so it becomes an opportunity for deeper scientific exploration.…”

Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan

“…Daun sungkai dibersihkan, dikeringkan, dan haluskan menggunakan grinder, dan disaring untuk menghasilkan simplisia (Muadifah et al, 2019a;Tarigan et al, 2020). Simplisia yang dihasilkan kemudian ditentukan randemen, parameter spesifik dan non-spesifik.…”

“…Pemeriksaan senyawa metabolit sekunder ekstrak dilakukan dengan mengacu kepada penelitian sebelumnya. Pengujian dilakukan terhadap senyawa alkaloid (pereaksi Meyer), steroid dan triterpenoid (Liebermann-Burchard), flavonoid (Mg dan amil alkohol), saponin (uji busa), tanin (FeCl3) (Tarigan et al, 2020). Skrining fitokimia terhadap senyawa saponin dilakukan dengan menambahkan air (1:1), dikocok selama 1 menit, pada pengujian ekstrak menimbulkan busa lalu ditambahkan HCl 1 N, dan busa yang terbentuk bertahan selama 1 menit dengan ketinggian 1 cm.…”

“…Skrining fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder di dalam daun sungkai. Pengujian dilakukan dengan cara menambahkan ekstrak kedalam tabung reaksi dan menambahkan reagen-reagen yang sesuai untuk pengujian pada masing-masing senyawa yang akan diamati, lalu dilihat perubahan atau kimia yang terbentuk setelah menambahkan reagen pada ekstrak mengacu pada penelitian sebelumnya (Muadifah et al, 2019b;Tarigan et al, 2020).…”

Isolation of alkaloids compound of ethanol extract of mangrove perepat (S. alba) root and its antibacterial activity

“…Tahap persiapan sampel dilakukan dengan mengikuti metode pada penelitian sebelumnya dengan modifikasi sebelumnya. Akar mangrove dikumpulkan, dibersihkan, dipotong kecil-kecil, dikeringkan, dan dihaluskan untuk mendapatkan simplisia (Muadifah et al, 2019;Muadifah et al, 2020).…”