Phytochemical screening and in vitro antioxidant activity of various Punica granatum l. Peel extracts from Algeria: A comparative study

Belkacem, Nacéra; Djaziri, R.; Lahfa, Farid; Haci, Imad Abdelhamid El; Boucherit, Zahia

doi:10.1007/s10298-014-0850-x

Cited by 24 publications

(18 citation statements)

References 36 publications

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“…Phytochemical studies indicate that the plant is rich in compounds like alkaloids, flavonoids, saponins tannins, phenols, cardiac glycosides and volatile oils. Our results are in good agreement with those reported by Belkacem et al while performing the phytochemical screening of various extracts of Punica granatum L [30]. The plant may possess antimicrobial activity due to the presence of any of these phytoconstituents which Figure 10: Zones of inhibition in millimeters at the highest concentration of 100 mg/ml of aqueous and methanolic extract of Solanum nigrum against different fungal strains.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Antibacterial, Antifungal and Phytochemical Screening of Solanum nigrum

Dar¹,

Bhat²,

Amin³

et al. 2017

Biochem Anal Biochem

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The present study aimed at evaluating the antimicrobial potential of aqueous and methanolic extracts of Solanum nigrum, a traditionally used medicinal plant with multiple therapeutic properties. The susceptibility of microbial strains to the two extracts was determined using agar well diffusion method. The bacterial strains employed were Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris and Escherichia coli. The fungal strains used were Penicillium Chrysogenum, Aspergillus fumigatus, Candida albicans and Saccharomyces cerevisiae. Phytochemical screening was performed using standard methods. A dose dependent increase in the antibacterial activity was observed with both the methanol and aqueous extracts. Highest antibacterial activity was exhibited by aqueous extract with Escherichia coli (16 ± 0.23 mm) followed by Staphylococcus aureus (15 ± 0.15 mm) at the concentration of 100 mg/ml plant extract. Methanolic extract showed highest antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with zone of inhibition (14 ± 0.11 mm) and (14 ± 0.26 mm) at the same concentration (100 mg/ml) respectively. The highest antifungal potential was exhibited by the methanolic extract against Saccharomyces cerevisiae (26 ± 0.27 mm) and Candida albicans (22 ± 0.13 mm), while the aqueous extract exhibits the highest antifungal potential against Saccharomyces cerevisiae (23 ± 0.14 mm) followed by Candida albicans (21 ± 0.10 mm) and Aspergillus fumigatus (16 ± 0.11 mm) at the concentration of 100 mg/ml. Phytochemical analysis revealed the plant is rich in various secondary metabolites like alkaloids, saponins, flavonoids, phenols and volatile oils. Cardenolides and phlobtannins were found absent. The study concludes that the plant possess novel compounds with significant antibacterial and antifungal properties. Isolation and characterization of these novel compounds could provide potent antimicrobial agents to combat pathogenic infections.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Antibacterial, Antifungal and Phytochemical Screening of Solanum nigrum

Dar¹,

Bhat²,

Amin³

et al. 2017

Biochem Anal Biochem

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“…By using Durbin-Watson statistic tests at (p < 0.05), relationships between overall acceptability and sensory characteristics were established. [45] and Barathikannan et al [46] reported an ethyl acetate TPC equal to 597.08 ± 3.9 and 218.152 ± 1.73 mg GAE/g of the extract, respectively. e ethyl acetate extract revealed the highest values of TFC with 104.128 ± 3.69 mg QE/g.…”

Section: Discussionmentioning

confidence: 93%

Variability in Phytochemical Contents and Biological Potential of Pomegranate (Punica granatum) Peel Extracts: Toward a New Opportunity for Minced Beef Meat Preservation

Fourati

Smaoui

Hlima

et al. 2020

Journal of Food Quality

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Extraction of Tunisian pomegranate peels was employed with different solvents such as ethyl acetate, acetonitrile, and water. Total phenolic and flavonoids contents, antioxidant activity, and antibacterial capacity against five foodborne pathogenic bacteria were evaluated. The highest values of polyphenols (351 mg gallic acid equivalent/g), flavonoids (104 mg quercetin/g), and DPPH and ABTS inhibition were recorded in the ethyl acetate extract followed by the aqueous extract. The latter present the maximum antibacterial potential against S. enterica, P. aeruginosa, and E. coli. The potential use of the lyophilized aqueous extract (AE), used for safety reason and being rich in phenolic, as biopreservative in minced beef meat was described. AE was incorporated at 0.1, 0.5, and 1% and compared with 0.1% butylated hydroxytoluene (BHT). During 21 days at 4°C, AE at 1% could appreciably retard the microflora proliferation (p<0.05), the accumulation of MetMb and the carbonyl group (p<0.05), slowing down the loss of sulphydryl proteins (p<0.05), and led to a decrease (p<0.05) in primary (peroxide value and conjugated dienes) and secondary lipid oxidation (TBARS) in treated meat. By the 14th day, AE-treated minced meat obtained higher sensory scores than untreated and BHT samples. Based on these results, lipid and protein oxidation changes and sensorial attributes were useful in discriminating meat samples by overall acceptability prediction. Generally, AE at 1% presented the potent preservative effect that could be utilized as an application on meat-substituting synthetic antioxidant.

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“…The calibration curve was plotted using gallic acid as a positive control. The results were expressed as gallic acid equivalent per gram of dry extract (mg GA E/g) [12].…”

Section: Methodsmentioning

confidence: 99%

Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species

Bourhia

Laasri

Moussa

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Ethnopharmacological Relevance Aristolochia baetica (A. baetica) and Aristolochia paucinervis (A. paucinervis) have been largely used in Moroccan folk medicine. The objective of the study was to investigate the phytochemical composition, the antioxidant activity, the antiproliferative effect, and the acute toxicity of the methanolic extract of A. baetica and A. paucinervis roots. Materials and Methods Phytochemical composition of the methanolic extract of A. baetica and A. paucinervis roots were studied using qualitative and quantitative methods, the antioxidant activity was evaluated using DPPH assay, the antiproliferative effects against human cancer cell lines (T-24, HT-29, and Hep G-2) was assessed using WST1 assay, and the acute toxicity was carried out orally by gavage of single dose 2000 mg/kg to mice for 14 days. Results The two studied plants have different classes of secondary metabolites. The concentrations of the total polyphenolic content of A. baetica and A. paucinervis root extracts were estimated at 360 ± 20 mg GAE/g and 280 ± 27 mg GAE/g, respectively. The total flavonoids content of A. baetica and A. paucinervis extracts were estimated at 35 ± 8 mg QE/g and 235 ± 7 mg QE/g, respectively. A. baetica and A. paucinervis extracts exhibited promising DPPH activity with IC50 values of 150 ± 8 μg/ml and 160 ± 10 μg/ml, respectively. The extracts exerted also antiproliferative effects on all tested cancer cell lines (T-24, HT-29, and Hep G-2) with IC50 values ranging from 6 ± 1 μg/ml to 380 ± 7 μg/ml. Regarding the results of acute toxicity study, no signs of toxicities nor mortalities were observed on the oral treated mice with 2000 mg/kg of the two investigated exacts. Conclusion The methanolic extracts of A. baetica and A. paucinervis possess several phytochemicals that exhibited promising free radical scavenging activity and antiproliferative effects.

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Phytochemical screening and in vitro antioxidant activity of various Punica granatum l. Peel extracts from Algeria: A comparative study

Cited by 24 publications

References 36 publications

Evaluation of Antibacterial, Antifungal and Phytochemical Screening of Solanum nigrum

Evaluation of Antibacterial, Antifungal and Phytochemical Screening of Solanum nigrum

Variability in Phytochemical Contents and Biological Potential of Pomegranate (Punica granatum) Peel Extracts: Toward a New Opportunity for Minced Beef Meat Preservation

Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species

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