Phytochemical Screening and In vitro Antimicrobial Properties of  Crude Leaf Extracts of Wrightia tinctoria R.Br.

Maddila, Sravanthi; Hemalatha, K.

doi:10.20546/ijcmas.2017.601.085

Cited by 8 publications

(7 citation statements)

References 22 publications

(14 reference statements)

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“…These functional groups in the extract are responsible for the reduction of precursor metal salt and the stabilisation of the AgNPs formed. The hydroxyl and carboxyl groups act as a reducing agent and depended on the modified nature of the extract, the size and morphological characteristics of the nanoparticles (Devi & Selvan 2017;Maddila & Hemalatha 2017;Singh et al 2018). Furthermore, the long-chain organic molecules would also help in the stabilisation of the AgNPs formed.…”

Section: Resultsmentioning

confidence: 99%

“…However, plant material is the most suitable substance for the green synthesis of nanoparticles (Jadoun et al 2020;Reda et al 2019). Plants naturally have compounds which may possess medicinal properties and these have been attributed to the phytochemicals, which are able to reduce, cap and stabilise Ag + ions (Jadoun et al 2020;Khatoon, Mazumder & Sardar 2017;Maddila & Hemalatha 2017). Plants and their extracts are simple to maintain, eco-friendly, rapid, affordable, easily available and their stability can be controlled (Hafez et al 2017;Vanlalveni et al 2021;Verma et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activities and biosynthesis of nanoparticles using hemp extracts

Singh

Coopoosamy

Naidoo

et al. 2022

JOMPED

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Background: The use of plants in the biosynthesis of nanoparticles is a fast-growing technique and has gained much interest from researchers over the years.Aim: This study reported the utilisation of leaf extracts of Cannabis sativa L. (hemp) for the biosynthesis of silver nanoparticles (AgNPs).Methods: In this study, techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) microanalysis and Fourier transform infrared (FT-IR) were carried out for the synthesis and characterisation of nanoparticles. The antibacterial efficacy of the synthesised nanoparticles was evaluated by using the agar diffusion and macrobroth dilution methods. The antibacterial properties of the biosynthesised AgNPs were evaluated against both Gram-negative and Gram-positive bacteria.Results: The formation of AgNPs was confirmed by colour change in plant extracts and further characterised by Ultraviolet-visible (UV-Vis) spectrophotometry TEM, SEM, EDX and FT-IR analyses. Fourier transform infrared analysis revealed the efficient capping and stabilisation properties of these particles and the nature of the capping agent. Silver nanoparticles prepared from leaf extracts showed effective antibacterial activity against Escherichia coli.Conclusion: Biosynthesised AgNPs showed a broad-spectrum antimicrobial susceptibility range and therefore represent promising antimicrobial agents. This is the first reported study for hemp leaf extracts and contributes to the environmentally friendly and cost-effective technique of biosynthesising nanoparticles for drug development.Contribution: This study contributes to the current medicinal properties of cannabis. Furthermore, it reflects that cannabis can also be utilized at a nanoscale with effective antibacterial efficacy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial activities and biosynthesis of nanoparticles using hemp extracts

Singh

Coopoosamy

Naidoo

et al. 2022

JOMPED

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“…In the last two decades, studies have been conducted with medicinal plants in different countries to prove their effectiveness as antimicrobial agents. Maddila and Hemalatha [80] report that global antibacterial resistance is becoming a growing public health problem, as bacterial resistance to most available antibacterial has been reported. e pharmaceutical industry and new biotechnology companies are intensifying efforts to discover new antibacterial in attempts to overcome bacterial resistance [80].…”

Section: Discussionmentioning

confidence: 99%

“…Maddila and Hemalatha [80] report that global antibacterial resistance is becoming a growing public health problem, as bacterial resistance to most available antibacterial has been reported. e pharmaceutical industry and new biotechnology companies are intensifying efforts to discover new antibacterial in attempts to overcome bacterial resistance [80]. In this sense, the crude extract of C. nutans leaves and their fractions (hexane, chloroform, ethyl acetate, and butanol) was tested, but only the hexane extract of the leaves was promising against the microorganisms (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

Souza

Gindri

Fortes

et al. 2020

Advances in Pharmacological and Pharmaceutical Sciences

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Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS.

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“…Medicinal plants are an abundant source of biologically active compounds. It is assumed that the bioreduction of nanoparticles using plant extracts is merely due to the presence of phytochemicals such as flavones, organic acids, polyphenols, and quinones [ 301 , 302 ]. The most frequently used metal nanoparticle for synthesizing plant constituents is silver [ 303 ].…”

Section: Synthesis Of Silver Nanoparticles From Plant Extracts Of Species Within Barleriamentioning

confidence: 99%

Phytochemicals and Biological Activities of Barleria (Acanthaceae)

Gangaram

Naidoo

Dewir

et al. 2021

Plants

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Plant species belonging to the family Acanthaceae are globally known to possess various medicinal properties and have cultural and economic importance in both traditional medicine and horticulture. They are important to both animals and humans and are used as food or for ornamental purposes worldwide. Barleria is the third largest genus in the family Acanthaceae. A few of the highly important and reported species of Barleria include B. prionitis, B. cristata, B. grandiflora, and B. lupulina. The flowers, leaves, stems, roots, and seed extracts of plants belonging to this genus are rich in bioactive compounds and have exhibited significant medicinal potential for the treatment of various ailments and infections. Evidence derived from several studies has demonstrated the antioxidant, antibacterial, antifungal, anti-inflammatory, anticancer, antidiabetic, antiulcer, hepatoprotective, analgesic, antiamoebic, antihelminthic, antiarthritic, antihypertensive, antiviral properties and toxicity of extracts, in addition inhibition of acetylcholinesterase activity and biosynthesis of nanoparticles, of the plant and seed extracts of species belonging to Barleria. Studies have reported that bioactive compounds such as flavonoids, quinones, iridoids, phenylethanoid glycosides, the immunostimulant protein “Sankaranin”, and antibiotics isolated from Barleria species are resposnsible for the above biological activities. Traditionally, the genus Barleria has significant medicinal potential; however, there is a scarcity of information on various species that are yet to be evaluated. This review provides a comprehensive report on existing literature, concerning the phytochemistry and biological activities of the genus Barleria.

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Phytochemical Screening and In vitro Antimicrobial Properties of Crude Leaf Extracts of Wrightia tinctoria R.Br.

Cited by 8 publications

References 22 publications

Antibacterial activities and biosynthesis of nanoparticles using hemp extracts

Antibacterial activities and biosynthesis of nanoparticles using hemp extracts

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

Phytochemicals and Biological Activities of Barleria (Acanthaceae)

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