Phytochemical Screening and HPTLC study of Padina tetrastromatica (Hauck)

Abstract

The marine ecology is diverse with innumerable types of natural substances, both of plant and animal origin. Padina tetrastromatica (PT) (Hauck) is a brown algae belonging to the order Dictyotales, found in coastal region. The objective of present investigation was to evaluate phytochemical profile of extracts of PT. The air dried plant material was defatted and extracted successively with solvents of increasing polarity. Incumbent study was performed with standard qualitative phytochemical tests and HPTLC fingerprint analysis using CAMAG HPTLC system. The results showed the presence of phytoconstituents like sterols, terpenoids, flavonoids, glycosides, alkaloids and carbohydrates. Furthermore, components present in extracts were resolved in best possible solvent system by HPTLC. The chloroform extract of PT displayed eight peaks, in which those with Rf values 0.28 and 0.72 were more predominant. Whereas ethanol extract of PT exhibited nine peaks, in which maximum Rf value was found to be 0.82. In conclusion, the data of this study provide useful guide and suitability for investigation of biological activity of the plant according to the phytochemical groups observed. However, further work is needed to standardize the above chemical constituents in comparison with biomarker and this result can also be measured along with the other data for setting up the standards to this plant.

Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh

“…Besides these, all components showed positive results in the methanolic extract of both seaweeds. Our present finding coincides with the findings of other authors; who also found several phytochemicals in case of altered solvents from brown seaweed P. tetrastromatica 20 , 33 . But in the case of G. tenuistipitata there has been no prior research on preliminary phytochemical screening was found in the literature.…”

“…The maximum significant application of seaweed is found in food industries, cosmetic industries, an industry of phycocolloid or hydrocolloid, biofuel production, wastewater treatment, pharmaceutical industry and fertilizer 18 , 19 . In general, seaweed contains different secondary metabolites, for example, tannins, saponins, phenols, and flavonoids in varying concentration 16 , 20 . In addition, seaweed has a wide range of bioactive compounds that have antibacterial, anti-Alzheimer’s, anti-inflammatory, antifungal, anti-hyperglycemic, anti-aging activities, and preferable antioxidant properties 21 – 24 .…”

Effect of Solvents on Bioactive Compounds and Antioxidant Activity of Padina Tetrastromatica and Gracilaria Tenuistipitata Seaweeds Collected From Bangladesh

“…Besides these, all components showed positive results in the methanolic extract of both seaweeds. Our present nding coincide with the ndings of other authors; who also found several phytochemicals in case of altered solvents from brown seaweed P. tetrastromatica 20,33,34 . But in the case of G. tenuistipitata there has been no prior research on preliminary phytochemical screening was found in the literature.…”

“…The maximum signi cant application of seaweed is found in food industries, cosmetic industries, an industry of phycocolloid or hydrocolloid, biofuel production, wastewater treatment, pharmaceutical industry and fertilizer 18,19 . In general, seaweed contains different secondary metabolites, for example, tannins, saponins, phenols, and avonoids in varying concentration 16,20 . In addition, seaweed has a wide range of bioactive compounds that have antibacterial, anti-in ammatory, antifungal, anti-aging activities, and preferable antioxidant properties 21,22 .…”