Phytochemical screening and evaluation of anti-fertility activity of Dactyloctenium aegyptium in male albino rats

Naik, B.Sreedhar; Dangi, Nim Bahadur; Sapkota, Hari Prasad; Wagle, Nabin; Nagarjuna, S.; Sankaranand, R.; Kumari, Beenta

doi:10.1016/j.apjr.2015.12.009

Cited by 19 publications

(5 citation statements)

References 14 publications

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“…The analysis was carried out following standard procedures as described by Sreedhar and colleagues [22]. Briefly, small portion of cauda epididymis was cut and crushed in 1 ml of 37 • C neutral buffer solution of sodium carbonate (NaHCO 3 ) to make a homogeneous mixture.…”

Section: Sperm Count and Fertility Analysismentioning

confidence: 99%

Sub-chronic toxicity evaluation of top three commercial herbal antimalarial preparations in the Kumasi metropolis, Ghana

et al. 2020

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Purpose: Safety data on commonly used herbal medicinal (HM) products (HMPs) and marketed in Ghana are scarce. We assessed the sub-chronic toxicity of three most-patronised commercial antimalarial HMPs in Kumasi, Ghana. Method: Top three HMPs (designated as herbal products ‘A’ (HPA), ‘B’ (HPB) and ‘C’ (HPC)) were selected after a mini-survey and sub-chronic toxicity evaluation conducted in accordance with Organisation for Economic Co-operation and Development (OECD) 407 guidelines. Control rats received clean water while test groups received daily adult human dose (DAHD), 5× DAHD or 10× DAHD of either HPA, HPB or HPC for 30 days. Rats were killed on day 31 to obtain biochemical, haematology and histology samples for analysis. Data were analysed by one-way analysis of variance (ANOVA) and post hoc Tukey’s test. Results: The three HMPs produced alterations in liver morphology predominantly characterised by prominent foci of fatty change with scattered hepatocytes containing intracytoplasmic fat globules and congested central veins and sinusoids. The lungs showed alveolar with evidence of inflammation and foci of epithelial sloughing. Alveolar spaces were also obscured by debris and inflammatory cells. HPA and HPC produced scattered intensely congested heart vessels while HPB(10) produced haemorrhage and amorphous exudates within the heart. All HMPs produced neither treatment-related deaths nor significant change in haematological and biochemical parameters, except for HPA and HPB which decreased (P<0.05) aspartate aminotransferase (AST) and HPB, which elevated (P<0.05) fasting blood glucose (FBG). Conclusion: Data from the present study suggest the potential of the herbal products (HPs), HPA, HPB and HPC, to cause major organ-system dysfunction or damage. We advise cautious use of these products and recommend further safety evaluation in chronic toxicity models.

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Section: Sperm Count and Fertility Analysismentioning

confidence: 99%

Sub-chronic toxicity evaluation of top three commercial herbal antimalarial preparations in the Kumasi metropolis, Ghana

et al. 2020

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“…Chemical and physicochemical assessment of D. aegyptium gave the following values (on dry basis): crude protein: 7.25%; fibre: 33.74%; N-free extract: 45.32%; ether extract: 1.23%; total ash: 12.46%; ash solubility in HCl: 8.65%; CaO: 0.91%; P2O7: 0.49%; MgO: 0.70%; Na: 0.074%; and K2O: 3.75% [30]. The plant contained carbohydrates, proteins, amino acids, terpenoids, alkaloids, saponins, tannins, flavonoids, steroids, fixed oils and phenols, according to the phytochemical study [31]. Carbohydrates, proteins, amino acids, saponins, flavonoids, and tannins were found in an aqueous extract.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

El-Sayed

Hussein²,

Elshamy³

et al. 2022

Egypt. J. Chem.

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The present study aimed to demonstrate the ameliorative impacts of Dactyloctenium aegyptium (D. aegyptium) and Parapholis incurva (P. incurva) alcoholic extracts on hormonal, histological, and ultrastructural changes in rat thyroid gland induced hypothyroidism by daily oral administration of sodium fluoride (NaF) (5 mg/kg) for 28 days. Forty-Two adult male albino rats were randomly divided into 6 equal groups (7 rats each): the control group, NaF group (5 mg/kg/d), D. aegyptium group (100 mg/kg/d), P. incurva group (100 mg/kg/d), NaF + D. aegyptium group and NaF + P. incurva group. At the end of the experiment, the animals were sacrificed and blood samples were subjected to hormonal assay for triiodothyronine (T3) and thyroxine (T4) and thyroid-stimulating hormone (TSH) levels. Malondialdehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD), and calcium were assessed. Specimens of thyroid gland tissues were processed for histological, histochemical, immunohistochemical and scanning electron microscopy examinations. Further chemical profiling of the both extracts were determined using HPLC. Phytochemically, 14 phenolic compounds including 10 phenolic and 4 flavonoids were identified from D. aegyptium while 13 phenolics were identified from P. incurva comprising 8 phenolic and 5 flavonoids. NaF induced significant alterations in hormonal (decrease in serum T3 and T4 levels and increase in serum TSH levels) and antioxidant status, as well as, calcium levels when compared with control group. Histopathological (many empty follicles lined by flat cells, hemorrhage and disrupted basement membrane), histomorphometric, histochemical, immunohistochemical and ultrastructural (thyroid calcification) alterations were also recorded, which all were markedly improved in groups that received D. aegyptium or P. incurva extract combined with NaF. D. aegyptium and P. incurva extracts are useful in combating thyroid gland calcification and toxicity induced by NaF due to their high flavonoids and phenolic compound content.

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“…A phytochemical analysis of the CGG root ethanol extract revealed the presence of large chemical groups, including alkaloids, tannins, saponins, and phytosterols [ 14 ]. A previous study reported that the administration of alkaloids and triterpenoids has a negative effect on fertility [ 36 ], as they can inhibit spermatogenesis [ 37 ] and alter the morphology and histology of testes [ 38 ]. These results are comparable with a study that administered Cissampelos capensis rhizomes , which caused a dose-dependent increase in sperm intrinsic superoxide production leading to sperm capacitation and DNA fragmentation [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Deleterious effect of short-term gavage of an ethanol extract of cogon grass (Imperata cylindrica L.) roots on testis and epididymal sperm quality

et al. 2020

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Background and Aim: Cogon grass (Imperata cylindrica L.) (CGG) is a herbal medicine that could be developed into a male antifertility agent. The present study aims to determine the effect of an ethanol extract of CGG roots on mice testicular activity, reproductive hormone levels, and epididymal sperm quality. Materials and Methods: This study was designed as completely randomized with three different doses, such as an ethanol extract of CGG roots at 0 (control), 90, and 115 mg/kg body weight. In total, 21 male DDY mice strain were treated with the CGG extract (by gavage) for 14 days, followed by an evaluation of reproductive organs, epididymal sperm quality, testis histology, histomorphometry, and reproductive hormone assays. All quantitative data were analyzed by analysis of variance, followed by Tukey's post hoc test at α=0.05. Results: The results showed that the administration of the CGG root ethanol extract disrupted the testis interstitial area and seminiferous tubules, resulting in decreased epididymal sperm quality as well as serum testosterone levels in a dose-dependent pattern. Conclusion: Oral administration of a CGG root ethanol extract induced testicular damage, decreased epididymal sperm quality, and impaired testosterone secretion.

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Phytochemical screening and evaluation of anti-fertility activity of Dactyloctenium aegyptium in male albino rats

Cited by 19 publications

References 14 publications

Sub-chronic toxicity evaluation of top three commercial herbal antimalarial preparations in the Kumasi metropolis, Ghana

Sub-chronic toxicity evaluation of top three commercial herbal antimalarial preparations in the Kumasi metropolis, Ghana

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

Deleterious effect of short-term gavage of an ethanol extract of cogon grass (Imperata cylindrica L.) roots on testis and epididymal sperm quality

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