Phytochemical Screening and Antibacterial Activity Coix lacryma-jobi Oil

Abstract

Coix lacryma-jobi (Hanjeli) is known to posses anti-microbial properties. Therefore, phytochemical compounds of C. lacryma-jobi have been studied to produce novel antimicrobial agents as treatments against antibiotic-resistant bacteria.The objective of this study was to determine the phytochemical composition and antibacterial activity of the C. lacryma-jobi oil against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The phytochemical composition of the oil was determined via gas chromatography mass spectrophotometry (GC-MS). Moreover, agar disk and agar well diffusion were employed to screen the antibacterial activity of the oil. An agar well diffusion test was implemented to determinate MIC's (minimum inhibitory concentrations). Dodecanoic acid, tetradecanoic acid, 2,3-dihydroxypropylester, 1,3-dioctanoin, N-methoxy-N-methyl-3,4-dihydro-2H-thiopyran-6-carboxamide, propanamide, 5-Amino-1-(quinolin-8-yl)-1,2,3triazole-4-carboxamide, and pyridine were identified in the C. lacryma-jobi oil. The MIC value of the oil was 0.031 g/L and the MBC of the oil was 0.125 g/L effective in all test bacteria. Dodecanoic acid displayed inhibitory activity against gram-positive and gram-negative bacteria. Therefore, our research demonstrated C. lacryma-jobi (Hanjeli) oil exhibited antibacterial activity against E. coli, S. aureus, and B. subtilis. These research suggest that C. lacryma-jobi root oil could be used for medicinal purposes; however clinical and in vivo tests must be performed to evaluate its potential as an antibacterial agent.

Effect of Ethanol Extract of Jamblang Aceh (Syzygium cumini) in Diabetic Mice (Mus musculus) and Its Potential As Anti-Diabetic Agent

“…Differences between groups were evaluated by one-way analysis of variance (ANOVA) followed by the Dunnet test to determine statistical significance. The significance level of this study was p <0.05 (Diningrat et al, 2020).…”

“…In this study, the experimental results showed significant anti-diabetic and antioxidant activity of S. cumini leaves ethanol extract at concentrations of 200 & 400 mg/kg bw. This study has been able to explore the potential of S. cumini leaves ethanol extract for the treatment of diabetes and related complications such as oxidant stress to prove the traditional claim that Jamblang leaves can be used for the diabetes treatment (Diningrat et al, 2020;Panigrahy et al, 2017). This study shows that an increase in glucose levels was successfully controlled by S. cumini leaves ethanol extract, this is indicated by a decrease in glycosylated hemoglobin levels commonly used as a control indicator of diabetes where glycohemoglobin levels approach normal values in diabetics in metabolic control (Volpe et al, 2018;Zimmet et al, 2017).…”

“…The anti-diabetic potential of S. cumini leaves ethanol extract is caused by the presence of secondary metabolites (flavonoids, alkaloids, phenolics, glycosides, and terpenes) contained in Syzygium cumini (Sari et al, 2018;Diningrat et al, 2020). These secondary metabolites are reported to have different anti-diabetic potential and are responsible for the observed anti-diabetic effects (Sari et al, 2018).…”

“…Preliminary Phytochemical Screening: Testing of constituents of phytochemical compounds contained in S. cumini ethanol extract was carried out using a method known as gas chromatography-mass spectrometer analysis (GCMS) (Diningrat et al, 2019;Diningrat et al, 2020).…”

“…Studies on the DM treatment have made great progress with the presence of oral hypoglycemic agents, but research on the hunt for new drugs continues because existing synthetic drugs have harmful side effects (Labay et al, 2016). Natural herbal medicines which are claimed as anti-diabetic agents, their availability in commercial formulations are still very limited (Srivastava et al, 2019;Diningrat et al, 2020).…”