Phytochemical properties and antibacterial activity of <i>Ageratum conyzoides, Piper betle, Muntinga calabura</i> and <i>Curcuma domestica</i> against mastitis bacteria isolates

Harjanti, Dian Wahyu; Ciptaningtyas, Rizke; Wahyono, Fajar

doi:10.1088/1755-1315/247/1/012049

Cited by 17 publications

(14 citation statements)

References 9 publications

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“…[51], rheumatoid arthritis in mice (preclinical) [52], antifungal [53], stress reducer in koi fishes [54], herbicide against weed Cyperus rotundus [55], anticancer in HeLa cells (in vitro) [56] also antidiabetic property in mice [57]. Phytochemical studies were validating the presence of saponins, alkaloids, steroids, flavonoids, phenols, tannins [58], also various essential oils in the class of Terpenes, Coumarins, and Chromeno [54]. Although this plant is also classified as weeds but the therapeutic ability exerted can outcompete and even benefit them in cultivation for medicinal purposes.…”

Section: Ethnomedicinal Research Across Indonesiamentioning

confidence: 99%

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

Brazia¹,

Adi²,

Kaho³

et al. 2020

Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)

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With at least 200 ethnic groups and 30.000 medicinal plants used in Jamu formulations, Indonesia is one of the largest natural drug dispensaries in the world. Ethnomedicine-directed bioprospecting has been used to accelerate drug discovery. Little is known about the coverage of ethnomedicinal studies in Indonesia. The quest to obtain drugs from plants also remains elusive. Nature-derived approved drugs worldwide have come mostly from drug-productive families that tend to be clustered in the phylogenetic tree, thus these families should be the basis of bioprospecting focus. We presented a review of ethnomedicinal research conducted across Indonesia and recommend strategic approaches to accelerate the translation of traditional knowledge into the clinic. We identified several plant species commonly used with similar or different purposes within different places, empirical pharmacological studies, regions that are understudied, and comparison with plants whose metabolites have been used for the development of approved medicine worldwide. Combination of ethnomedicinal knowledge with molecular phylogenetics,-omics, Bioactivity-guided bioprospecting, DNA barcoding, and Global Biodiversity Information Facility (GBIF) software will enable more efficient and environmentally friendly drug discovery process as well as commercial production. Altogether, this proposed method will be a game-changer in the exploration of the largely untapped chemical diversity of Indonesian flora.

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Section: Ethnomedicinal Research Across Indonesiamentioning

confidence: 99%

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

Brazia¹,

Adi²,

Kaho³

et al. 2020

Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)

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“…Hasil penelitian serupa juga dilaporkan oleh Zhan et al [29] [3,30]. Jumlah sel somatis juga mempunyai keterkaitan dengan aktivitas enzim di dalam susu yang dapat mempengaruhi stabilitas dan daya simpan susu.…”

Section: Materi Dan Metodeunclassified

“…Manifestasi penyakit mastitis dibedakan menjadi dua macam, yaitu mastitis klinis dan mastitis subklinis. Kasus subklinis merupakan yang terbesar, yaitu mencapai 80% dan tidak disertai dengan gejala klinis yang nampak pada mammary, serta tidak terjadi perubahan fisik pada susu [3]. Hal ini menyebabkan peternak seringkali tidak mengetahui bahwa ternaknya menderita penyakit mastitis subklinis.…”

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Tingkat peradangan mammary dan stabilitas susu sapi mastitis subklinis yang mendapat suplemen herbal dan Zn-Se proteinat

et al. 2020

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Mastitis subklinis adalah peradangan mammary tanpa disertai perubahan fisik susu, namun menyebabkan perubahan kimiawi susu. Penelitian ini bertujuan untuk mengetahui tingkat peradangan mammary dan stabilitas susu sapi mastitis subklinis yang mendapatkan suplemen pakan herbal daun papaya dan kunyit serta Zn-Se proteinat. Metode: Penelitian menggunakan 16 ekor sapi mastitis subklinis. Rancangan penelitian berupa rancangan acak lengkap dengan 4 perlakuan dan 4 ulangan. Pakan yang digunakan adalah rumput gajah dan konsentrat. Perlakuan berupa : To (pakan basal); T1 (pakan basal + suplemen daun pepaya dan kunyit 0,03% BB); T2 (pakan basal + Zn-Se propionate (Se 0,5 mg/kgBB dan Zn 60 mg/kgBB)); dan T3(pakan basal + suplemen daun pepaya dan kunyit 0,03% BB + Zn-Se propionate (Se 0,5 mg/kgBB dan Zn 60 mg/kgBB)). Data dianalisis dengan analisis ragam dan perbedaan efek perlakuan diuji dengan Duncan's Multiple Range Test. Hasil: Kelompok T1, T2 dan T3 memiliki tingkat peradangan mammary lebih rendah (P<0,05) dibandingkan T0. Peradangan intra-mammary menurun (P<0,05) seiring lamanya waktu perlakuan. Tingkat peradangan yang paling rendah ditunjukkan oleh kelompok T3 (P<0,05). Nilai pH susu setelah 8 jam penyimpanan di suhu ruangan pada kelompok T1, T2, T3 lebih tinggi (P<0,05) dibandingkan dengan T0. Pengujian stabilitas susu dengan uji alkohol dan uji didih menunjukkan hasil 100% negatif pada kelompok T3 hari ke 12, sedangkan T1 dan T2 pada hari ke 15. Kesimpulan: Kombinasi suplemen herbal daun papaya dan kunyit serta Zn-Se propionat dapat digunakan untuk terapi mastitis subklinis pada sapi perah untuk menghasilkan susu yang lebih sehat dan memperbaiki stabilitas susu.

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“…Some preclinical studies determined the antimicrobial activities of herbs using several bioassays such as disk diffusion, well diffusion, broth agar, and agar dilution methods [ 10 , 11 ]. In addition, some previous studies successfully conducted the antibacterial susceptibility test of various single herbal extracts against mastitis-causing bacteria, which were isolated from mastitis milk [ 4 ]. The most persistent problem in preclinical studies is the contamination due to bacteria, fungus, and mold, which, perhaps, arises while executing stages of extraction and following experimental procedures.…”

Section: Introductionmentioning

confidence: 99%

“…The antibacterial efficacy was tested against mastitis-causing bacteria, which was isolated from mastitis-infected cows. Notably, tetracycline, erythromycin, and penicillin, which are commonly used for mastitis therapy in dairy farms [ 4 , 15 , 16 ], were used as standard antibiotics in this study.…”

Section: Introductionmentioning

confidence: 99%

Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria

2020

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Background and Aim: The current phytobiotic industry is struggling to determine a proper sterilization method for an herbal formula that comprises multiple plant extracts. Hence, this study aims to investigate the effects of two sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacterial isolates. Materials and Methods: The herbal formula comprised the extracts of Piper betle leaves, Curcuma domestica, and Curcuma zanthorriza. We applied two sterilization methods – sterilization using 0.45 μM syringe filter and sterilization using an autoclave at 121°C for 15 min. After sterilization, we measured phytochemical contents (phenol and flavonoid) of the herbal formula. Using the disk diffusion method, the antibacterial susceptibility test of the sterilized herbal formula against mastitis-causing bacterial isolates was conducted. Tetracycline, erythromycin, and penicillin – common antibiotics for mastitis therapy in dairy farms – were used as standard antibiotics. Results: Compared with autoclave sterilization, syringe filter sterilization resulted in less (p<0.05) phenolic and flavonoid contents. Against Escherichia coli and Staphylococcus aureus, the filter sterilized herbal formula (E. coli: 65.9%-73%; S. aureus: 6.2%-18.1%) markedly reduced the antibacterial activity than the autoclave-sterilized herbal formula (E. coli: 2.1%- 3%; S. aureus: 4.5%-10.7%). Conclusion: This study establishes that autoclave sterilization of the herbal formula is the best sterilization method that exerts minimal adverse effects on the phytochemical compounds and could sustain the antibacterial efficacy against mastitis-causing bacteria. Hence, the herbal formula could be used as an alternative treatment for bovine mastitis.

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Phytochemical properties and antibacterial activity of Ageratum conyzoides, Piper betle, Muntinga calabura and Curcuma domestica against mastitis bacteria isolates

Cited by 17 publications

References 9 publications

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

Upgrading Indonesian Local Ethnomedicinal Knowledge with Molecular Phylogenetics

Tingkat peradangan mammary dan stabilitas susu sapi mastitis subklinis yang mendapat suplemen herbal dan Zn-Se proteinat

Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria

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