Phytochemical profiles, antioxidant and antimicrobial activities of three Potentilla species

Abstract

BackgroundExtracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species (Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia) in order to discover new resources for lead structures and pharmaceutical products.MethodsChemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride/chloranil-based method (SBC); Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method.ResultsThe results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 (P. fruticosa), 2.56 (P. glabra) and 2.68 mg/g (P. parvifolia), respectively. The highest content of total identified phenolic compounds (hyperoside, (+)-catechin, caffeic acid, ferulic acid, rutin and ellagic acid) was observed in P. parvifolia (14.17 mg/g), follow by P. fruticosa (10.01 mg/g) and P. glabra (7.01 mg/g). P. fruticosa possessed the highest content of total phenolic (84.93 ± 0.50 mmol gallic acid equivalent/100 g) and total flavonoid (84.14 ± 0.03 mmol quercetin equivalent/100 g), which were in good correlation with its significant DPPHIC50 (16.87 μg/mL), ABTS (2763.48 μmol Trolox equivalent/g) and FRAP (1398.70 μmol Trolox equivalent/g) capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg/mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC50 and MIC values of 20.52–47.02 mg/mL and 0.78–50 mg/mL, respectively.ConclusionsThese results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.

A Modified and Improved Assay Based on Microbial Test System (MTS) to Evaluate Antioxidant Activity

“…In vitro Antioxidant Activity of Pure Compounds, Fruits, and Vegetables DPPH Radical Scavenging Assay DPPH radical scavenging was monitored according to the method of Wang et al (2013). A 2.0 ml of test compound solutions (0.003-0.2 mM) or extract solutions (0.39-100 mg ml −1 ) was added to 2.0 ml of DPPH solution (0.1 mM).…”

Potentilla and Rubus medicinal plants

“…Due to the lack of Rubus genome sequences, over 70,000,000 86-nt Illumina high-throughput sequencing reads derived from R. idaeus roots were aligned to the Fragaria vesca genome (Ward et al, 2012). idaeus (red raspberry) is one of the important economical crops that possess numerous nutrients, micronutrients, and phytochemicals with essential health benefits to human.…”

Synergistic effects and related bioactive mechanism of Potentilla fruticosa L. leaves combined with Ginkgo biloba extracts studied with microbial test system (MTS)

“…In China, the plant species P. fruticosa also called “Jinlaomei” and “Gesanghua”, has long been utilized as folk herbs especially in traditional Tibetan medicine for its superior therapeutic effects [4, 6]. The leaf extracts of P. fruticosa were used to prevent cardiac-cerebral vascular disease, the stem extracts of P. fruticosa were used to treat inflammation, dyspepsia and edema in traditional Tibetan medicine [7–9].…”

“…In consideration of the rational basis for the use of phytochemicals in P. fruticosa and EGb were still poorly explored by chemical methods including DPPH, ABTS, FRAP, and the oxygen radical absorbance capacity (ORAC) assay, a strategy to enhance the physiological bioavailability of P. fruticosa combined with EGb seems to be imperative [4, 24–26]. According to the results of previous study, the combination of P. fruticosa and EGb showed enhanced activity in achieving a superior synergism outcome directed at the free radical oxidant damage.…”