Phytochemical profile of methanolic extracts from Chilca (Baccharis glutinosa) roots and its activity against Aspergillus ochraceus and Fusarium moniliforme

Abstract

The present study aimed to identify the phytochemicals of methanolic extract from Baccharis glutinosa (chilca) roots (MEBg) and to evaluate its antifungal activity on two major fungal pathogens of agricultural importance. The antifungal activity was evaluated by inhibition halo, minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and % sporulation against A. ochraceus and F. moniliforme. As-1 a preliminary test, inhibition halo was tested using 1, 10, 100 and 270 mg ml of MEBg. Different concentrations of MEBg were applied for MIC and MFC tests. Ketoconazole was used as positive control. The treatments were applied in triplicate. The phytochemical compounds of MEBg were determined by GC-MS analysis. The MEBg produced an inhibition zone of 2 to 4 mm in the inhibition halo test, with concentrations-1 of 100 and 270 mg ml for A. ochraceus and F. moniliforme, respectively. Reduction in % sporulation above-1 50 was shown in concentrations over 8 mg ml. MEBg were reported to exhibit antifungal activities against A.-1 ochraceus and F. moniliforme with the MIC values ranging from 2 to 5.6 mg•ml and the MFC from 12 to 15-1 mg ml. GC-MS analysis of Chilca extracts revealed that the most abundant metabolites were furfural compounds and organic acids. The most abundant furfural compounds were 5-(hydroxymethyl) furan-2carbaldehyde (38.59%), furan-2-carbaldehyde (4.103%) and 5-methylfuran-2-carbaldehyde (2.1%). The MEBg revealed efficient antifungal activity, likely due to the presence of bioactive compounds, which could be used as an alternative for biological control of pathogenic fungi in maize and coffee crops.

Antifungal Activity of Root Extracts from Baccharis salicina on Germination of Uredospores of Hemileia vastatrix

“…On the other hand, the presence of furans 2, 5furandicarboxylic acid, dendrolasin (3-(4,8-dimethyl-3,7nonadienyl)-furan) and 3-hydroxy-2-phenyl-2,3,3a,7atetrahydrofuro[3,2-b]pyran-5-one, coincide with previous work by Lam-Gutiérrez et al (2019) where this molecule class was found via GC-MS, albeit with adifferent molecular mass. (2E,6E)-2-(4-methylpent-3-en-1-yl)-6-[3-(2,5-dihydro-2-oxofuran-3-yl)-propylidene]hept-2-enedioic acid was found in aerial parts from B. thymifolia (Hikawczuk et al 2008).…”

“…The roots of B. salicina were dried at room temperature, ground to a fine powder, and sifted using a 2 mm mesh sieve. Subsequently, 300 g of pulverized roots were macerated in 3 L of methanol, as reported by Lam-Gutiérrez et al (2019).…”

Anticancer, antioxidant, antiviral and antimicrobial activities of Kei Apple (Dovyalis caffra) fruit

“…Anticancer activity was reported using methyl-5-(hydroxymethyl)-2-furan carboxylate and its derivatives against Vero cell HeLa and HepG2 lines 29 . Another study observed that the proliferation of bacteria was inhibited by furan compounds 30 . Levoglucosenone, sesquiterpene lactone (isochiapin B) and dotriacontane were recognized as constituents of Kei apple extract (Table 2 ), and their cytotoxic effects against hepatocarcinoma cell lines 31 , antioxidant activity 32 , and antibacterial and antiviral activities 33 were documented.…”

Revealing Berberis aristata potential as an anti-viral agent to combat Paramyxoviridae infection

“…The gas chromatography mass spectroscopy process performed enabled in identifying the compounds present in the extract of B. aristate, while dealing with moieties of different polarities that needs to be identified, it is suggested to separate them to hexanoic and methanolic fractions (Lam-Gutiérrez et al, 2019). Based on the retention time and the highest peaks obtained in the chromatogram, six compounds were identified based on retention time.…”