“…First identified by Gerber in 1903 as identical to affinin 1, spilanthol has been mostly found in Acmella flowers, leaves, and stems [4,7,8,[12][13][14][15][16], but also in roots [17,18], and its accumulation in in vitro cell cultures has been documented [2,3]. Due to its pharmacological importance, based on a series of effects typical of alkylamides, such as analgesic, neuroprotective, antioxidant, antimutagenic, anti-cancer, anti-inflammatory, antimicrobial, anti-larvicidal, and insecticidal activities [19], many protocols for spilanthol production and extraction have been developed (see [19] for a review; [20]). Besides recent in vivo treatments with biostimulants [21], a good approach to obtain a great number of plants, in a short period of time under selected standardized conditions of cultivation, is the use of in vitro plant cultures [2,3,8,[22][23][24][25][26][27].…”