Phytochemical Profile, Antioxidant and Antitumor Activities of Green Grape Juice

Abstract

(1) Plants, due to their phytochemicals, have long been known for their pharmacological potential and medicinal value. Verjuice, the acidic juice of unripe green grape, is still poorly characterized in terms of its chemical composition and biological activities. (2) In this study, we characterized the chemical composition, antioxidant and antitumor potential of verjuice extract. Folin–Ciocalteu and aluminum chloride reagents were used to identify the total phenol and total flavonoid composition. Various conventional methods were used to quantify the alkaloids and tannins. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and Neutral Red assay were used to assess the antioxidant and antitumor activities, respectively. (3) We showed that the verjuice extract contains alkaloids, tannins, and a high quantity of total flavonoids and total phenols. Besides its antioxidant activity, verjuice significantly repressed human pulmonary adenocarcinoma (A549) cells’ viability in both dose- and time-dependent manners. Moreover, verjuice extract significantly enhanced the anticancer potential of cisplatin. (4) Altogether, these observations suggest a potential use of verjuice as a natural antitumor remedy.

Antioxidant and Cytotoxic Activities of Usnea barbata (L.) F.H. Wigg. Dry Extracts in Different Solvents

“…In five volumetric flasks of 25 mL, 2 mL of each solution B1-B5 were added, with 1 mL of Folin-Ciocâlteu reagent, 10 mL water, and 290 g/L of Na 2 CO 3 solution, up to the mark; a blue coloration resulted in each volumetric flask. After 30 min of reaction at room temperature [ 62 ], the absorbencies (each value was noted with A1 in the calculation formula) were determined at 760 nm, using a Jasco V630 UV-Vis Spectrophotometer (Japan) with Spectra Manager™ Software.…”

Potency of Combining Eucalyptus camaldulensis subsp. camaldulensis with Low-Dose Cisplatin in A549 Human Lung Adenocarcinomas and MCF-7 Breast Adenocarcinoma

“…Numerous plant species are as of now being utilized to treat or anticipate the advancement of cancer. Several analysts have recognized species of plants that have illustrated anticancer properties [13]. Eucalyptus is one of those medicinal plants widely distributed in Australia.…”

Caftaric Acid Isolation from Unripe Grape: A “Green” Alternative for Hydroxycinnamic Acids Recovery

“…An alternative use of unripe grapes is the production of verjuice, an acidic juice traditionally produced in the Mediterranean area, which is extracted from the mechanical pressing of unripe green grapes. Verjuice has been mainly studied for its physicochemical and sensory properties [ 21 , 22 ], while its bioactive compounds were just recently isolated [ 23 , 24 ]. In grape berries, HCAs are constituted by caffeic, coumaric, and ferulic acids and are present mainly in their ester forms, associated with tartaric acid giving caftaric (CFA), coutaric (CUA), and fertaric (FEA) acids, respectively [ 25 ].…”