Phytochemical profile and antioxidation activity of annona fruit and its effect on lymphoma cell proliferation

Abstract

Introduction Lymphoma is a cancer of the lymph nodes and lymphatic system. The two main types are Hodgkin and non‐Hodgkin. Non‐Hodgkin lymphoma is the most common. The lymphomas can be effectively treated with chemotherapy; however, the treatments have severe side effects that can be permanent. Studies have shown a relationship between a high intake of vegetables/fruits and reduced risk of cancer development, including lymphomas. Hypothesis We hypothesize that Annona fruit, which is rich in fibers and phytochemicals, can be very effective in inhibiting lymphoma cancer growth. Experimental The skin, pulp and seeds fractions of Annona fruits were extracted into water, methanol or chloroform and then assayed for their total phenolic contents and anti‐oxidation activities and then tested for cell proliferation inhibitory activity using Ramos‐1 lymphoma cell line. Results The highest concentration of total phenols was present in the skin fraction; however, the pulp has the highest total amounts. The methanol fractions of skin and pulp and water extract of seeds contained the highest anti‐oxidation activity. The methanol extracts of skin, pulp and seeds have moderate whereas chloroform extracts of pulp and seeds have strong inhibition of Ramos‐1 lymphoma cell proliferation. Conclusions The edible pulp of Annona fruits can provide phenolic anti‐oxidants which can also be effective in preventing/treating lymphoma. The skin and seeds are non‐edible but can be used to isolate potent compounds with potential anti‐cancer activity. Future Directions Studies are required to isolate and identify the active compounds from Annona fruit and to test them in an animal model. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

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Immature ginger reduces triglyceride accumulation by downregulating Acyl CoA carboxylase and phosphoenolpyruvate carboxykinase-1 genes in 3T3-L1 adipocytes

“…After treatment, cells were scraped and collected in a 15 μL centrifuge tube. Cells were washed 1× with PBS by centrifugation at 2,000 × g , and the pallets were used for the determination of fatty acid composition using gas chromatography as described previously ( 67 ).…”

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