Phytochemical investigation of saponifiable matter & volatile oils and antibacterial activity of Moluccella laevis L., family Lamiaceae (Labiatae)

Abstract

The current study was aimed to evaluate some parts of Moluccella laevis viz., phytochemical and antibacterial. Regarding to GC/MS of the saponifiable matters of petroleum ether fraction of total ethanolic extract of the aerial parts (TEE), the main recognized unsaturated fatty acids were methyl linolenate (25.58%) and methyl linoleate (15.87%). Whereas, the major saturated fatty acids were, palmitic (25.4%) followed by stearic acid (10.48). A comparative analysis of the volatile constituents of M. laevis flowers and leaves was performed by Head Space GC/MS. The volatile mixtures of both plant parts displayed comparable amounts of hydrocarbons and oxygenated compounds, with a noticeable greater contribution of the latter in the leaves.Besides, -pinene (40.84%), chrysanthenyl acetate (17.89%) and isobornyl acetate (10.64%), were identified as the major volatile components in the flowers. While, isobornyl acetate (35.09%) was characterized as the major constituent followed by, 2-methyl-4-butanolide (22.12%), 1-heptene oxide (7.47%) and benzoic acid, methyl ester (4.05%) of the volatile oil composition of the leaves. Moreover, this study included the antibacterial activity of TEE and its different fractions against Gram positive and negative bacteria. TEE exhibited MIC values of 326 476, and 541 µg/mL against E. coli, K. pneumonia and P. aeruginosa, respectively, while the aqueous fraction showed

Spathulenol as the most abundant component of essential oil of Moluccella aucheri (Boiss.) Scheen

“…Based on the molecular analysing investigations, M. aucheri is suggested as a sister of the two species M. laevis L. and M. spinosa L. (Scheen & Albert, 2009). Recent GC-MS analysis of the essential oil (EO) of the flowers of M. laevis exhibited α-pinene, chrysanthenyl acetate, and isobornyl acetate as the major constituents, while isobornyl acetate, 2-methyl-4-butanolide, 1-heptene oxide and methyl benzoate were detected in the oil of the plants' leaves (Hamed et al, 2020). In another attempt, the EOs' constituents of M. laevis were characterized by GC-MS as α-pinene, pinocarvone, methyl chavicol and β-caryophyllene (Shehata, 2001).…”

Biological potential of Moluccella laevis L. aerial parts, family Lamiaceae (Labiatae)

“…The volatile oils and the saponifiable matters of the petroleum ether (Pet. ether) fraction of the total ethanolic extract (TEE) of M. laevis were analyzed by GC-MS [3,4]. Moreover, the antibacterial activity of TEE and its different fractions was investigated against Gram-positive and Gram-negative bacteria [4].…”

“…ether) fraction of the total ethanolic extract (TEE) of M. laevis were analyzed by GC-MS [3,4]. Moreover, the antibacterial activity of TEE and its different fractions was investigated against Gram-positive and Gram-negative bacteria [4]. Additionally, both the aqueous and EtOAc fractions of TEE demonstrated the most potent cytotoxic effect against immortalized cell lines of human colorectal adenocarcinoma (CACO-2) and human breast cancer cell lines (MCF-7), respectively [5].…”

Micromorphological features of stem, root and inflorescence of Moluccella laevis L., Family Lamiaceae (Labiatae)

“…One important plant of this genus is Molucella laevis L. due to its chemical and biological values. The essential oils and the saponifiable matters of the petroleum ether fraction of the total ethanolic extract (TEE) of M. laevis were analysed by GC-MS [3,4]. Moreover, the antibacterial activity of TEE and its various fractions was studied against Gram-positive and Gram-negative bacteria [4].…”

“…The essential oils and the saponifiable matters of the petroleum ether fraction of the total ethanolic extract (TEE) of M. laevis were analysed by GC-MS [3,4]. Moreover, the antibacterial activity of TEE and its various fractions was studied against Gram-positive and Gram-negative bacteria [4]. Furthermore, both of the aqueous and EtOAc fractions of TEE demonstrated the most potent cytotoxic effect against cancer cell lines as CACO-2 and MCF-7, respectively [5].…”