Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

Alaa, M.; Kadhim, Enas Jawad

doi:10.31351/vol29iss2pp27-36

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Preliminary phytochemical analysis was carried out for methanolic fraction using Mayer's reagent (alkaloids), 5% ethanolic KOH (flavonoids), FeCl3 (tannins), foam test for saponins, Benedict's test for sugars (17,18) , and Ninhydrin test for proteins (19) .…”

Section: Preliminary Chemical Testsmentioning

confidence: 99%

Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice

Jaafar,

Ali F Hassan,

Maha N Hamad

2023

IJPS

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The aim of the study was extraction of arial part of Euphorbia cyathophora constituents with methanol and evaluate its effect on mitotic index and total chromosomal aberration bone marrow cell and spleen cell in mice 200 gm of E. cyathophora fine powder was defatted then extracted by cold maceration 80% ethanol for seven days. The extract was filtered and dried in a rotary evaporator then the dried extract was suspended with water and consecutively extracted using chloroform, ethyl acetate for each. The aqueous layer was then mixed with 100ml methanol. These fractions are dried under reduced pressure to obtain the dry extract. Twenty-four Albino mice were used for the experiment. The animals were divided into four groups: Group 1: Mice were treated with distilled water. The dose was given daily for seven successive days. Group 2: Mice were treated with a single dose (20mg/kg) of methotrexate (positive control). Group 3: Mice were treated with (100mg/kg) of menthol fraction for seven successive days. Group 4: Mice were treated with (200mg/kg) of methanol fraction for seven successive days. Mice were sacrificed by (spinal dislocation). Samples of bone marrow cells and spleen cells were taken and genotoxic analyses. Methanol fraction of Euphorbia cyathophora at a dose of 100mg/kg demonstrated a significant decrease in mitotic index and a significant increase in total chromosomal aberrations as compared to distilled water in both bone marrow cells and spleen cells (p<0.05). 100 mg/kg and 200 mg/kg of methanol fraction of Euphorbia cyathophora that showed to be significantly higher in mitotic index and significantly lower in total chromosomal aberration as compared to methotrexate (p<0.05). In conclusion the current study revealed that the methanol fraction of aerial parts of Euphorbia cyathophora is genotoxic but its genotoxicity is less than that of methotrexate.

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Section: Preliminary Chemical Testsmentioning

confidence: 99%

Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice

Jaafar,

Ali F Hassan,

Maha N Hamad

2023

IJPS

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“…At 1453 cm -1 appears absorption band from OH vibrations of planar distortion. In the area of 1375.94 cm-1 appears a characteristic ribbon, which derives from CH3 group and at 1049.5 cm -1 stretching vibrations of C-O group of secondary alcohol (20). the characteristic IR absorption bands of the isolated compound are listed in Table 2 and Fig.…”

Section: Characterization Of Isolated Uamentioning

confidence: 99%

Isolation, characterization and quantification of a pentacyclic triterpinoid compound ursolic acid in Scabiosa palaestina L. distributed in the north of Iraq

Khamees

Kadhim

2021

Plant Sci. Today

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Ursolic acid (UA, 3 ?-hydroxy-urs-12-en-28-oic acid) are isomeric triterpenic acids. The high quantities of pentacyclic triterpenoids in Scabiosa species seems to be obvious and there is an evidence that most of pentacyclic triterpenoids that have been isolated are saponins. This is one of the most important characteristic of the genus Scabiosa, the main aglycones are ursolic acid and oleanolic acid. In the current study, isolation from the aerial part and roots of Scabiosa palaestina L. was performed using Preparative HPLC. Furthermore, detection and quantitation of ursolic acid was performed by high performance thin layer chromatography (HPTLC). The identification of isolated triterpenoid involves two methods including FT-IR coupled with LC-MS/MS that have been used for the simultaneous determination of the isolated UA. Quantitative analysis of Ursolic acid content in chloroform fractions revealed that both of the aerial parts and roots contain comparable concentration of 0.052 and 0.054 mg/ml respectively. The FT-IR and LC-MS/MS spectra of the isolated compound shows good agreement with those reported in literatures of Ursolic acid. Quantitative concentration of UA in chloroform fraction revealed that aerial parts and roots contain comparable concentrations and the spectral data for the isolated unknown were in good agreement with those reported in literature of UA.

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The effect of aqueous extract and tannins from the Lepidium draba plant on the bacteria contaminating restaurants in the city of Sharqat

Shehab¹,

Mostafa

Saeed

et al. 2022

1st Samarra International Conference for Pure and Applied Sciences (Sicps2021): Sicps2021

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Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

Cited by 3 publications

References 20 publications

Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice

Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice

Isolation, characterization and quantification of a pentacyclic triterpinoid compound ursolic acid in Scabiosa palaestina L. distributed in the north of Iraq

The effect of aqueous extract and tannins from the Lepidium draba plant on the bacteria contaminating restaurants in the city of Sharqat

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