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This study included collecting 100 samples in the form of gum swabs, gum pockets, saliva, and extracted teeth, and PCR technology was used to determine the incidence of Entamoeba gingivalis in northern Salah El-Din governorate for healthy and people with oral diseases and those suffering from diabetes of the first and second types. The overall injury rate was 52%. It was found that the incidence of people with oral diseases was 60%, and the rate was 33.3% in healthy subjects. And that the rate of infection in persons with gingivitis is the highest rate of infection among oral diseases, as the rate reached 80% and the lowest incidence of 30% for those with tooth decay disease. The incidence of oral diseases and those suffering from diabetes is 61%, and the incidence of persons with oral diseases and those suffering from type 1 diabetes is the highest incidence of persons with oral diseases and those suffering from diabetes type II, as the rate reached 66.6, 55.5%, respectively. And the incidence of smokers and those with oral diseases is higher than that of persons with oral diseases and non-smokers, as the rate was 78.5 and 47.6%, respectively.