Phytochemical identification of volatile fraction, essential oil and screening of antioxidant, antibacterial, allelopathic and insecticidal potential from Artemisia herba-alba leaves

Abstract

In the present paper, the essential oil and the volatiles emitted in vivo by leaves of A. herba alba, which grows wild in center of Tunisia, were analyzed by GC-MS and SPME, respectively. The essential oil was characterized by the predominance of oxygenated monoterpenes (93.3%) with α-thujone as the major compound (45.5%) followed by β-thujone (11.4%), trans-sabinyl acetate (10.1%) and 1,8-cineole (7.4%). The SPME analyses showed that oxygenated monoterpenes were the most representative compounds (64.9%). The major ones were α-thujone (37.9%), germacrene D (16.5%), 1,8-cineole (8.4%) followed by β-thujone (7.8%). Moreover, different activities (antioxidant, antibacterial, allelopathic and insecticidal) of the leaf essential oil were investigated. The antioxidant activity was ascertained by evaluating its inhibitory effect against DPPH and ABTS. The results showed that the essential oil had a strong activity and it was more effective against the DPPH radical (IC50 = 6μg/mL) than the ABTS radical (IC50 = 40μg/mL). In antibacterial screening, it inhibited the growth of all bacteria tested except Salmonella anatum and Salmonella salamae which were resistant to this oil type. Staphylococcus aureus and Vibrio cholera were found to be extremely sensitive with the lowest MIC value (0.007 mg/mL). In addition, allelopathic activity was evaluated against Lactuca sativa and Raphanus sativus. Data showed that, with essential oil, seed germination was completely inhibited for both of them, suggesting a potent allelopathic potential for the evaluated oil. Insecticidal activity was investigated against Culex pipiens larvae and a weak activity (LC50 241.1 ppm) for this A. herba alba essential oil

Chemical Composition and Antimicrobial Activity of Artemisia herba-alba and Origanum majorana Essential Oils from Morocco

“…aureus , E. coli , and B. cereus [23,32,33]. Moreover, several studies showed a great potential of A. herba-alba EO oil as an antibacterial agent against Klebsiella pneumoniae , Listeria monocytogenes , Vibrio colerae , and S. Typhimurium [34,35,36]. Our results showed variable antimicrobial and antifungal activity of the essential oil, being the inhibition zones in the range of 10–24 mm.…”

Extraction Yield, Phytochemicals Analysis and Certain In-Vitro Biological Activities of Artemisia herba alba Extracts

“…The following results have been identified as not sensitive for diameters of ≤ 8mm, sensitive for diameters of 8-14mm, very sensitive for diameters of 14-20mm, and extremely sensitive for diameters of ≥20mm (Bellili et al, 2017). L. monocytogenes ATCC 7644 and S. aureus ATCC 29213 were both significantly (p ≤ 0.05) sensitive to methanolic extract, with L. monocytogenes ATCC 7644 showing the greatest inhibition activity (13.00 mm).…”

The Genus Artemisia: a 2012–2017 Literature Review on Chemical Composition, Antimicrobial, Insecticidal and Antioxidant Activities of Essential Oils

“…The pronounced antioxidant activity may be due to the phenolic constituents. A. campestris oil showed maximal DPPH activity at dose of 2 mg/mL [ 110 ], however, A. herba - alba oil showed strong DPPH activity (IC 50 6 μg/mL) than ABTS assay (IC 50 40 μg/mL) [ 72 ]. In another study, IC 50 values of A. turanica oil reported were 7.00 mg/mL, 9.69 μg and 14.63 μg, in DPPH, nitric oxide and superoxide anion radicals, respectively.…”