PHYTOCHEMICAL EXAMINATION OF<i>DRACAENA MANNII</i>STEM BARK

Sofowora, E. A.; Olaniyi, A. A.

doi:10.1055/s-0028-1097761

Cited by 7 publications

(3 citation statements)

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“…Phytochemical screening of the individual aqueous herbal extracts was performed using previously published protocols [22][23][24][25][26][27][28]. The Frothing test for saponins [29]; Fehling's test for reducing sugars [29]; the Ferric chloride test for phenolic compounds, polyuronides, and cyanogenic glycosides; Mayer's test for alkaloids; Shinoda's test for flavonoids; and the Salkoski test for triterpenes and phytosterols were performed [29]. The presence of anthracenosides was also determined using a protocol described in Benmehdi et al's study [25].…”

Section: Culturing Of Plasmodiummentioning

confidence: 99%

The In Vitro Antiplasmodial Activities of Aqueous Extracts of Selected Ghanaian Herbal Plants

Cudjoe

Donu

Okonu

et al. 2020

Journal of Parasitology Research

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Background. The asexual and sexual stages (gametocytes) of Plasmodium falciparum parasites are known to respond differently to antimalarial drugs. Herbal products with extended treatment regimens and inadequate dosing information are widely used to treat malaria in Ghana. This study set out to determine the in vitro activity of selected herbal extracts on the development of asexual and sexual stage malaria parasites. Methods. The 72-hour SYBR Green 1-based in vitro drug assay was used to determine the asexual parasite growth inhibitory effects exhibited by aqueous extracts of Alchornea cordifolia, Polyalthia longifolia, Moringa oleifera, and Mangifera indica on the NF54, CamWT_C580Y, and IPC 4912 strains of Plasmodium falciparum. The effects of exposure of asexual and early-stage NF54 gametocytes to varying concentrations of the aqueous herbal extracts were assessed by microscopy after 7 days of continuous culturing in the presence of the herbal extract. Qualitative and quantitative phytochemical screening were also performed on the herbal extracts. Results. In the SYBR Green 1 assay, aqueous extracts of Alchornea cordifolia exhibited moderate (IC50 of 5.8, 17.4, and 15.8 μg/ml) and Mangifera indica exhibited low (IC50 of 65.4, 96.7, and 81.7 μg/ml) activities against the NF54, Cam WT_C580Y, and IPC 4912 parasites, respectively, whilst Polyalthia longifolia and Moringa oleifera were inactive. Long-term treatment of NF54 parasites with 1 mg/ml of Polyalthia longifolia produced the highest densities of gametocytes and the least (56%) inhibition of asexual parasites on Day 7. Long-term treatment of NF54 parasites with 10 μg/ml Alchornea cordifolia resulted in complete parasite (asexual and gametocyte) clearance on Day 7. Conclusions. Alchornea cordifolia exhibited a ‘moderate’ activity against the three parasites tested in the 72-hour SYBR Green 1 assay and also effectively cleared both asexual parasites and gametocytes. Long-term treatment of malaria parasites with herbal extracts mimics a treatment regimen and should be used to determine the antimalarial properties of herbal extracts.

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Section: Culturing Of Plasmodiummentioning

confidence: 99%

The In Vitro Antiplasmodial Activities of Aqueous Extracts of Selected Ghanaian Herbal Plants

Cudjoe

Donu

Okonu

et al. 2020

Journal of Parasitology Research

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“…Evaporation of the solvent under reduced pressure (40 • C) provided the extracts. A preliminary phytochemical screening investigated the presence of alkaloids (Dragendorff"s test), saponins (foam formation), flavonoids (diphenylboryloxyethylamine-polyethylene glycol reagent), terpenoids (Liebermann-Bouchardat's test) and tannins (FeCl 3 ) according to standard methods (Sofowora and Olaniyi, 1975;Wagner and Bladt, 1996).…”

Section: Extraction and Preliminary Phytochemical Investigationmentioning

confidence: 99%

Direct and indirect antimicrobial effects and antioxidant activity of Cordia gilletii De Wild (Boraginaceae)

Okusa

Penge

Devleeschouwer

et al. 2007

Journal of Ethnopharmacology

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“…The chemical compounds isolated from Bryophyllum pinnatum are used as folk medicines against many ailments such as hypertension and kidney stones, (Lans, 2006) pulmonary infections, rheumatoid arthritis, etc (Quaziet al, 2011). Bryophyllum pinnatum leaves are used as an antifungal (Sofowora, 1993) and anti-allergic agent (Pal et al,1991). The oxidative activity was detected by DPPH and Nitric oxide free radical scavenging method.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Lectin Isolated From Leaves of Bryophyllum Pinnatum

Devi¹,

Garg²,

Kumar³

et al. 2021

PLANT ARCHIVES

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The Bryophyllum pinnatum refers to the family Crassulaceae. The glycoprotein from the 0.9% of normal saline extracts of Bryophyllum pinnatum leaves was refined by dialysis with 50% of ammonium sulfate. Protein concentration was invented by Lowry’s method. The dialyzed sample allowed for SDS-PAGE to determine the molecular weight (Mr ). The antioxidant activity of crude lectin extract has been assessed by the DPPH (1, 1-Diphenyl-2-picrylhydrazyl) and ABTS methods. The present study aims to verify the antioxidant activity of lectins detached from the leaves of Bryophyllum pinnatum.

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PHYTOCHEMICAL EXAMINATION OFDRACAENA MANNIISTEM BARK

Cited by 7 publications

References 1 publication

The In Vitro Antiplasmodial Activities of Aqueous Extracts of Selected Ghanaian Herbal Plants

The In Vitro Antiplasmodial Activities of Aqueous Extracts of Selected Ghanaian Herbal Plants

Direct and indirect antimicrobial effects and antioxidant activity of Cordia gilletii De Wild (Boraginaceae)

Antioxidant Activity of Lectin Isolated From Leaves of Bryophyllum Pinnatum

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