Phytochemical Composition and Anticancer Activity of Seaweeds Ulva Lactuca and Eucheuma Cottonii Against Breast McF-7 and Colon HCT-116 Cells

Abstract

Objective: This study is aimed to develop marine resources, which is focused on the determination of phytochemical composition and exploration of seaweeds Ulva lactuca and Eucheuma cottonii, as a potential antibreast cancer and anticolorectal cancer agents.Methods: Seaweeds U. lactuca collected from Parangtritis beach, Yogyakarta, Central Java, Indonesia, whereas E. cottonii collected from Salemo Island, South Sulawesi, Indonesia. Seaweeds U. lactuca and E. cottonii were macerated in organic solvents, n-hexane, chloroform, ethyl acetate, and ethanol, respectively. After maceration for 3 days, the mixture was filtered; the filtrate was concentrated by rotary evaporator. The concentrated extract of n-hexane, ethyl acetate, ethanol, and chloroform was then analyzed by thin layer chromatography. Phytochemical test of the concentrated extract was conducted to identify the metabolites containing in the seaweeds. Furthermore, the cytotoxic activity of the n-hexane, ethyl acetate, ethanol, and chloroform extract of U. lactuca and E. cotonii were evaluated as a growth inhibitor of breast MCF-7 and colorectal HCT-116 cancer cells by MTT cell proliferation assay.Results: Phytochemical test for the concentrated extracts of U. Lactuca showed the positive result for metabolites of steroids, glycosides, flavonoids, and tannins, whereas the concentrated extracts of E. cottonii showed the positive result for metabolites of steroids, glycosides, and flavonoid. Both concentrated extracts of U. lactuca and E. cotonii exhibited anticancer activity against breast MCF-7 and colorectal HCT-116 cells with IC 50 ranging from 21 µg/mL to 99 µg/mL. Conclusion:Our results clearly demonstrate seaweeds U. Lactuca and E. cotonii as promising candidates for the new antibreast and anticolorectal cancer agents.

Free Radical Scavenging Activity of Marine Sponges Collected From Kovalam, Chennai

“…The purified L-asparaginase showed good antioxidant activity on DPPH assay and provided a potential anticancer activity against MCF-7 cell line and should be considered for further pharmaceutical use as anticancer agents [38]. The results clearly demonstrate seaweeds Ulva lactuca and Eucheuma cottonii as promising candidates of new anti breast and anticolorectal cancer agents [39]. Pigmented rice serves as a good sources of antioxidant compounds, red rice varieties showed higher antioxidant properties and has health promoting properties as well as anti-cancer properties [40].…”

Phytochemical Test and Cytotoxic Activity of Macroalgae Eucheuma cottonii against Cervical HeLa Cells

“…8,19 Our previous research on red seaweed Euchema cottonii derived from South Sulawesi, Indonesia that demonstrated a strong cytotoxic activity against breast MCF-7 and colorectal HCT-116 cells, also containing metabolite of flavonoids. 10 Therefore, the mechanism of action of flavonoids as antioxidant and anticancer is a strong point of discussion in this work. Antioxidant systems are proposed to be involved in carcinogenesis because of its capability to damage the reactive oxygen species.…”

“…Method of extraction and fractionation of seaweed is adopted from previous research, 10 in which, 1000 g dry powder of macroalgae E. cottonii were macerated in ethyl acetate in a sealed glass vessel for a week, with conditional stirring. After maceration process, the filtrates were evaporated to give concentrated extract of Eucheuma cottonii.…”

Phytochemical Profile, Antioxidant Activity and Cell Line Study of Marine Red Macroalgae Eucheuma cottonii on Lung A-549 Cancer Cells

“…Maceration and extraction technique of Eucheuma cottonii is adopted 10 , as follows. Eucheuma cottonii is dried and ground to form a powder.…”

“…Some previous researchers has reported that Eucheuma cottonii demonstrated anticancer activity against MCF-7 breast cancer cells, HCT-116 colorectal cancer cells, and HeLa cervical cancer cells. [10][11][12][13] Beside that, Eucheuma cottonii are also widely cultivated in Indonesia, including in West Papua, with production level reached 6549 tons per year. 14 The anticancer activity and the large production of Eucheuma cottoni iin Indonesia has encouraged us to carry out phytochemical analysis, antioxidant activity, and cell line study of nutritious Eucheuma cottonii against lung A-549 cancer cells.…”