Free Radical Scavenging Activity of Marine Sponges Collected From Kovalam, Chennai

Thambidurai, Yuvarani; Dorairaj, Sudarsanam; Mohideen, Habeeb Shaik; T, Ashok Kumar

doi:10.22159/ajpcr.2017.v10i6.18115

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…Natural products, such as marine sponges, commonly showed antioxidant properties [20,21]. Some marine sponge-derived natural products showed both cytotoxic and antioxidant activities [22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Manoalide Preferentially Provides Antiproliferation of Oral Cancer Cells by Oxidative Stress-Mediated Apoptosis and DNA Damage

Wang

Tang

et al. 2019

Cancers

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Marine sponge-derived manoalide has a potent anti-inflammatory effect, but its potential application as an anti-cancer drug has not yet been extensively investigated. The purpose of this study is to evaluate the antiproliferative effects of manoalide on oral cancer cells. MTS assay at 24 h showed that manoalide inhibited the proliferation of six types of oral cancer cell lines (SCC9, HSC3, OC2, OECM-1, Ca9-22, and CAL 27) but did not affect the proliferation of normal oral cell line (human gingival fibroblasts (HGF-1)). Manoalide also inhibits the ATP production from 3D sphere formation of Ca9-22 and CAL 27 cells. Mechanically, manoalide induces subG1 accumulation in oral cancer cells. Manoalide also induces more annexin V expression in oral cancer Ca9-22 and CAL 27 cells than that of HGF-1 cells. Manoalide induces activation of caspase 3 (Cas 3), which is a hallmark of apoptosis in oral cancer cells, Ca9-22 and CAL 27. Inhibitors of Cas 8 and Cas 9 suppress manoalide-induced Cas 3 activation. Manoalide induces higher reactive oxygen species (ROS) productions in Ca9-22 and CAL 27 cells than in HGF-1 cells. This oxidative stress induction by manoalide is further supported by mitochondrial superoxide (MitoSOX) production and mitochondrial membrane potential (MitoMP) destruction in oral cancer cells. Subsequently, manoalide-induced oxidative stress leads to DNA damages, such as γH2AX and 8-oxo-2’-deoxyguanosine (8-oxodG), in oral cancer cells. Effects, such as enhanced antiproliferation, apoptosis, oxidative stress, and DNA damage, in manoalide-treated oral cancer cells were suppressed by inhibitors of oxidative stress or apoptosis, or both, such as N-acetylcysteine (NAC) and Z-VAD-FMK (Z-VAD). Moreover, mitochondria-targeted superoxide inhibitor MitoTEMPO suppresses manoalide-induced MitoSOX generation and γH2AX/8-oxodG DNA damages. This study validates the preferential antiproliferation effect of manoalide and explores the oxidative stress-dependent mechanisms in anti-oral cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Manoalide Preferentially Provides Antiproliferation of Oral Cancer Cells by Oxidative Stress-Mediated Apoptosis and DNA Damage

Wang

Tang

et al. 2019

Cancers

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“…The FRAP method is the only method that measures antioxidants and reductants in a sample directly, while other methods measure antioxidant activity indirectly by measuring free radical inhibition produced in the solution and the results depend on the reactive species being used [18,[21][22][23]. Therefore, the value of the result of the reduction in FRAP is determined by the value of EC 50 ( Fig.…”

Section: Alkaloid Testmentioning

confidence: 99%

Fractionation of the N-Hexane Extract of Garcinia Bancana Miq. (Manggis Hutan) Leaves and Its Antioxidant Activity Based on 1,1-Diphenyl-2- Picrylhydrazyl and Ferric Reducing Antioxidant Power Assays

2018

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Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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Free Radical Scavenging Activity of Marine Sponges Collected From Kovalam, Chennai

Cited by 2 publications

References 26 publications

Manoalide Preferentially Provides Antiproliferation of Oral Cancer Cells by Oxidative Stress-Mediated Apoptosis and DNA Damage

Manoalide Preferentially Provides Antiproliferation of Oral Cancer Cells by Oxidative Stress-Mediated Apoptosis and DNA Damage

Fractionation of the N-Hexane Extract of Garcinia Bancana Miq. (Manggis Hutan) Leaves and Its Antioxidant Activity Based on 1,1-Diphenyl-2- Picrylhydrazyl and Ferric Reducing Antioxidant Power Assays

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