Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of <i>Hedyotis diffusa</i> in <i>Caenorhabditis elegans</i>

Li, Jing; Liu, Di; Dan-qing, LI; Guo, Yujie; Cao, Yanjun

doi:10.1002/cbdv.202100685

Cited by 5 publications

(2 citation statements)

References 41 publications

(34 reference statements)

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“…The nematodes treated by SCPs were lysed with double distilled water in an ice bath after 7 days. The activities of SOD, MDA, and CAT content were determined ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Sea cucumber (Acaudina leucoprocta) peptides extended the lifespan and enhanced antioxidant capacity via DAF-16/DAF-2/SOD-3/OLD-1/PEPT-1 in Caenorhabditis elegans

Yang²,

Xu³

et al. 2022

Front. Nutr.

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The sea cucumber peptides (SCPs) from Acaudina leucoprocta were derived from the patented bio-enzyme digestion technology and the molecular weight of obtained SCPs was < 10 kDa. In this study, we investigated the possible anti-aging effects of SCPs on the model of Caenorhabditis elegans and the underlying mechanisms. SCPs extend the average lifespan of nematodes by 31.46%. SCPs enhance the anti-stress capacity of C. elegans by improving heat resistance and mobility, Also, the accumulated potential oxidative stress inducers like lipofuscin and reactive oxygen species (ROS) were reduced to 40.84 and 71.43%. In addition, SCPs can increase the antioxidant capacity in nematodes by enhancing the activity of SOD and CAT and reducing MDA accumulation in nematodes to 32.44%. Mechanistically, SCPs could mediate DAF-16/DAF-2/SOD-3/OLD-1/PEPT-1 axis to improve antioxidant capacity and extend lifespan in nematodes. Taken together, these findings provide a direction for the anti-aging effects of sea cucumber peptides and new insights into the further purifications of SCPs and future research on aging.

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“…The nematodes treated by SCPs were lysed with double distilled water in an ice bath after 7 days. The activities of SOD, MDA, and CAT content were determined ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Sea cucumber (Acaudina leucoprocta) peptides extended the lifespan and enhanced antioxidant capacity via DAF-16/DAF-2/SOD-3/OLD-1/PEPT-1 in Caenorhabditis elegans

Yang²,

Xu³

et al. 2022

Front. Nutr.

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“…Just to name two examples: for instance, when resveratrol is added to worms at L1 stage, it extends lifespan. [190][191][192] However, if resveratrol is added later in life at L4 or adult stage, it is reported to have highly variable outcomes with either a signicant lifespan extension or not. 148,[193][194][195][196] Another example is naphtazarin treatment, which in concentrations of up to 500 mM causes a lifespan extending effect on solid media, 197 while in liquid culture it exerts a potent nematotoxic effect already at 79 mM.…”

Section: Nps In C Elegans-based Drug Screeningmentioning

confidence: 99%

The role of Caenorhabditis elegans in the discovery of natural products for healthy aging

et al. 2023

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Comparative study of Scleromitrion diffusum and Oldenlandia corymbosa: Microscopy, TLC, HPLC, and antioxidant activity

Ho,

Au,

et al. 2024

Microscopy Res & Technique

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Quality control of herbal medicines is crucial, especially the role of herbal drug identification. This is essential for preventing the misuse of herbs, which can affect efficacy or cause toxicity. Scleromitrion diffusum is a common herb, yet it is often mistaken for Oldenlandia corymbosa. This study analyzed the morphology, microscopy, thin‐layer chromatography (TLC), and high‐pressure liquid chromatography (HPLC) using two markers, asperuloside and scandoside methyl ester, to distinguish between S. diffusum and O. corymbosa with the analysis included 10 samples of S. diffusum and 10 samples of O. corymbosa collected from the Taiwan market. By quantifying the total polyphenols and flavonoids, we investigated the antioxidant activity, including the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging effect, 2,2′‐azino‐bis 3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS•+) scavenging effect, and reducing power to further elucidate the biological effects of the two herbs. The results of this study revealed notable differences in microscopy and suggested a TLC method for distinguishing between the two herbs in the market. In HPLC, the ratios of asperuloside and scandoside methyl ester differed between the two herbs. S. diffusum contained a higher asperuloside content. In contrast, O. corymbosa contained higher concentrations of scandoside methyl esters. With more total polyphenols and flavonoids in S. diffusum than those in O. corymbosa, the antioxidant activity of S. diffusum was superior to that of O. corymbosa. This study provides a comprehensive understanding for the identification and quality evaluation of S. diffusum in the market.Research Highlights The study consolidates and clarifies the morphological and microscopic differences between Scleromitrion diffusum and Oldenlandia corymbosa – a common adulterant species of S. diffusum on the Taiwan markets. Using Asperuloside and Scandoside methyl ester as two chemical markers, the study proposes a TLC method for rapidly testing S. diffusum and O. corymbosa on the market. Through HPLC analysis, our results showed that S. diffusum and O. corymbosa had a clear difference in the ratio of two markers, Asperuloside and Scandoside methyl ester: Asperuloside/Scandoside methyl ester in S. diffusum is higher than that in O. corymbosa. Through phytochemicals contents, including total phenols content, flavonoids content, and antioxidant activity, including DPPH, ABTS•+ scavenging activity, and reducing power, S. diffusum showed slightly higher levels of phenols and flavonoids as well as a better antioxidant activity than O. corymbosa.

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Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans

Cited by 5 publications

References 41 publications

Sea cucumber (Acaudina leucoprocta) peptides extended the lifespan and enhanced antioxidant capacity via DAF-16/DAF-2/SOD-3/OLD-1/PEPT-1 in Caenorhabditis elegans

Sea cucumber (Acaudina leucoprocta) peptides extended the lifespan and enhanced antioxidant capacity via DAF-16/DAF-2/SOD-3/OLD-1/PEPT-1 in Caenorhabditis elegans

The role of Caenorhabditis elegans in the discovery of natural products for healthy aging

Comparative study of Scleromitrion diffusum and Oldenlandia corymbosa: Microscopy, TLC, HPLC, and antioxidant activity

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