Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of <i>Leptadenia hastata</i> (pers) Decne Leaves: In Silico and In Vitro Studies

Chukwuma, Ifeoma Felicia; Nworah, Florence; Apeh, Victor Onukwube; Omeje, Kingsley O.; Nweze, Ekene John; Asogwa, Chukwudi Daniel; Ezeorba, Timothy Prince Chidike

doi:10.1177/11779322221115436

Cited by 16 publications

(1 citation statement)

References 106 publications

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“…Generally, the UBL family of proteins covalently modifies specific proteins to their functional state. Moreover, a recent study found UBF-1 to play a crucial role in immune modulation and inflammatory responses, especially for diabetic conditions [ 49 – 51 ]. Since our in silico analysis gave a plausible tentative clue to UBF-1 possessing immune modulatory peptides (Fig.…”

Section: Discussionmentioning

confidence: 99%

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

et al. 2023

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Background Despite the invasiveness of the Hepatitis B infection, its vaccines are only formulated with FDA-approved alum-based adjuvants, which poorly elicit a lasting immune response, hence the need for a more effective adjuvant system. This study evaluated the immunogenicity and toxicity of eggshell membranes (ESM) when administered as an adjuvant for the recombinant HBV vaccine (rHBsAg) in albino mice. Differential white blood cell analysis, as well as the titer measurement of Immunoglobulin G, subclass G1 and G2a on indirect ELISA, was performed to measure the immune-modulatory potentials of ESM. Moreover, analysis of the liver marker enzyme (AST and ALT) and body/liver weights was performed to ascertain the toxicity level of ESM. Finally, Immuno-informatic analysis was used to investigate the immune-modulatory potential of individual member proteins of ESM. Results Our results showed a significant improvement in the experimental group's lymphocyte count after boost-dose administration compared to the controls, whereas there was no significant change in the granulocyte population. Furthermore, the formulations (ESM-rHBsAg) significantly improved IgG and IgG1 titers after each successive immunization. Body/liver weight and liver function showed ESM non-toxic to mice. The immunoinformatic analysis discovered ovalbumin, lysozyme-C, and UFM-1 as the member proteins of ESM with immune-modulatory activities of activating antigen-presenting cells (APC). Conclusion This study has provided a clue into the potential valorization of eggshell membranes and their peptides as an adjuvant for vaccines such as HBV. We recommend more in-depth molecular analysis to support our findings as well as foster real-life application.

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Section: Discussionmentioning

confidence: 99%

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

et al. 2023

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Exploring the potential of aqueous extracts of Artemisia annua ANAMED (A3) for developing new anti‐malarial agents: In vivo and silico computational approach

Apeh,

Okafor,

Chukwuma

et al. 2023

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The emergence of resistance to current antimalarial drugs poses a significant challenge in the fight against malaria. This study aimed to investigate the in vivo antiplasmodial potential of the aqueous extract of fresh and dried leaves of A3 in Plasmodium berghei‐infected (P. berghei) mice. A 4‐day suppressive test was conducted, with infected BALB/c mice receiving artesunate and A3 extracts. The results showed that the tested doses of A3 attenuated the elevation of parasitemia induced by P. berghei, particularly at the dose of 400 mg/kg, and improved hematological indices. Computational techniques, including molecular docking, binding free energy calculations, and ADMET predictions, identified several bioactive compounds in A3 with promising inhibitory potential against lysyl‐tRNA synthetases and Dihydrofolate reductase (DHFR), the crucial enzymes targeted by antimalarial drugs. In this paper, Friedelin, Bauerenol, Epifriedelanol, Alpha‐Amyrenone, Stigmasterol, and beta‐Amyrin acetate were top‐ranked, having docking scores from −10.6 to −9.9 kcal/mol, compared with the −9.4 and −7.1 kcal/mol demonstrated by artesunate and chloroquine, respectively, as standard ligands. Also, it was shown that docking score from the Lysyl‐tRNA protein target (4YCV) ranged from −9.5 to −7.8 kcal/mol in comparison to artesunate (8.1 kcal/mol) and chloroquine (5.6 kcal/mol). The results suggest that the identified compounds in A3 could serve as potential candidates for the development of new anti‐malarial agents.

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Phlorotannins from brown algae: a review on their antioxidant mechanisms and applications in oxidative stress-mediated diseases

et al. 2023

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Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of Leptadenia hastata (pers) Decne Leaves: In Silico and In Vitro Studies

Cited by 16 publications

References 106 publications

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

Exploring the potential of aqueous extracts of Artemisia annua ANAMED (A3) for developing new anti‐malarial agents: In vivo and silico computational approach

Phlorotannins from brown algae: a review on their antioxidant mechanisms and applications in oxidative stress-mediated diseases

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