Phytochemical Characterization, Bioactivities Evaluation and Synergistic Effect of Arbutus unedo and Crataegus monogyna Extracts with Amphotericin B

Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits

“…A strong correlation between TPC and DPPH (r = 0.929 and −0.976 for RSA measured as TE and EC50, respectively) indicated that phenolic compounds were responsible for the radical scavenging properties of A. unedo leaves and fruits. This correlation between the phenolic composition of extracts and their respective antioxidant activity has already been reported previously [ 10 , 12 , 13 , 23 , 36 ].…”

“…Previous studies concerning the composition of phenolics in A. unedo leaves usually included a limited number of compounds [ 8 , 9 , 11 , 26 ], and there is a general lack of literature data concerning complete phenolic profiles. Only a few studies were carried out with regard to the phenolic profiling of A. unedo leaves using HPLC-MS [ 8 ], HPLC-MS/MS [ 10 , 11 , 12 ], or LC-HRMS [ 13 , 23 ], techniques that enable the unambiguous identification of phenolic compounds. These authors determined 12–40 phenolics in leaves, which was far less than the 60 phenolics identified in leaves in our study.…”

“…Considering all of the above, there is a need to expand the knowledge on the phytochemicals in A. unedo in order to discover new natural antioxidant sources. According to the literature, until now there have been only studies that determined the limited numbers of phenolic compounds in leaves (12–40 phenolics) [ 8 , 9 , 10 , 11 , 12 , 13 ] and fruits (8–42 phenolics) [ 7 , 8 , 9 , 10 , 11 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ] using different analytical techniques such as gas chromatography-mass spectrometry (GC-MS) [ 14 ]; high-performance liquid chromatography (HPLC) coupled with either mass spectrometry (MS) [ 7 , 10 , 11 , 12 , 15 , 16 , 19 , 21 ], high-resolution MS (HRMS) [ 13 ], or diode array detection (DAD) [ 9 , 16 , 19 , 20 , 22 ]; and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) [ 18 ]. Among all phenolic compounds, flavonoids were the dominant compounds.…”

“…Among all phenolic compounds, flavonoids were the dominant compounds. Apart from a general lack of literature data concerning complete phenolic profiles, only a few studies were carried out with regard to the phenolic profiling of A. unedo leaves using HPLC-MS [ 8 ], HPLC-MS/MS [ 10 , 11 , 12 ], or LC-HRMS [ 13 , 23 ], techniques that enable the unambiguous identification of phenolic compounds.…”

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

“…At the same time, these extracts showed no cytotoxic effects on healthy colon epithelial cells [ 45 ] and normal human peripheral mononuclear cells [ 46 ]. Additionally, the anti-oxidant activity of the phenolic composition and individual compounds from Crataegus pinnatifida berries [ 47 ], Crataegus monogyna leaves [ 48 ], and Crataegus oxyacantha flowers and berries [ 49 ] were described. The recent reports showed mild cytotoxic effects of phenolic acids from Petroselinum crispum L. on human U87MG cells [ 22 ], flavonoids from Lippa graveolens on human U251MG cells [ 23 ], and anthocyanins from Rubus liebmannii and Rubus palmeri on C6 and RG2 rat and murine, respectively, glioma cell lines [ 50 ].…”